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|Application / Reactivity||Human|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||2 Antibodies|
|Enzyme Immunoassay (EIA)||8 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies|
|Functional Studies (Func)||6 Antibodies|
|Immunoassay (IA)||2 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies|
|Immunofluorescence (IF)||10 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||18 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||16 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Protein Assay (PrA)||2 Antibodies|
|Western Blotting (WB)||199 Antibodies|
|Antigen||Erythropoietin (EPO) Antibodies|
|Epitope||AA 1-166 Alternatives|
|Conjugate||This EPO antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
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Product Details anti-EPO AntibodyTarget Details EPO Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Erythropoietin(EPO) detection. Tested with WB, IHC-P, ELISA, IP in Human.
Gene Name: erythropoietin
Protein Name: Erythropoietin
|Purification||Immunogen affinity purified.|
|Immunogen||E. coli-derived human EPO recombinant protein(Position: A1-R166).|
Target Details EPOProduct Details anti-EPO Antibody Application Details Handling Images back to top
|Alternative Name||EPO (EPO Antibody Abstract)|
|Background||EPO, Erythropoietin, is an acidic glycoprotein hormone with a molecular mass of 34 kD. It is a cytokine for erythrocyte(red blood cell) precursors in the bone marrow. This gene is mapped to 7q22.1. It is produced by interstitial fibroblasts in the kidney in close association with peritubular capillary and tubular epithelial cells. It is also produced in perisinusoidal cells in the liver. While liver production predominates in the fetal and perinatal period, renal production is predominant during adulthood. As the prime regulator of red cell production, its major functions are to promote erythroid differentiation and to initiate hemoglobin synthesis. It also has other known biological functions. For example, erythropoietin plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process.|
|Research Area||Cancer, Organogenesis, Hematopoietic Progenitors, Angiogenesis|
Application DetailsProduct Details anti-EPO Antibody Target Details EPO Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Species: Human
IP: Species: Human
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
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|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
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