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anti-Human EPO antibody for Immunoprecipitation

Recommended EPO Antibody (supplied by: Log in to see )

Antigen
Erythropoietin (EPO) Antibodies
  • EP
  • EPO
  • MVCD2
Epitope
AA 1-166
21
18
16
13
13
10
8
5
5
5
5
3
3
3
3
2
2
2
1
1
1
1
Reactivity
Human
284
52
31
5
2
1
1
Host
Rabbit
166
139
4
3
3
1
Clonality
Polyclonal
Conjugate
This EPO antibody is un-conjugated
25
19
14
8
6
6
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
217
207
21
18
11
11
10
8
6
6
6
4
3
3
3
2
2
1
1
Supplier
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Catalog No. ABIN3042813
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN2450538 IP IHC (p) ELISA WB Rabbit IgG AA 1-166 Log in to see Polyclonal
0.00089556724 ABIN2262168 IP IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.00089556724 ABIN2141409 IP IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.00089556724 ABIN2156947 IP ELISA WB Mouse IgG1 Log in to see NYRhEPO-
0.00089556724 ABIN3030866 IP IHC (p) ELISA WB Rabbit IgG AA 1-166 Log in to see Polyclonal
0.00089556724 ABIN645602 IP IHC ELISA WB Mouse IgG1 Log in to see

Similar anti-EPO Antibodies

Application / Reactivity Human
ELISA 192 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 3 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Functional Studies (Func) 6 Antibodies
Immunoassay (IA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 18 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Neutralization (Neut) 1 Antibodies
Protein Assay (PrA) 2 Antibodies
Western Blotting (WB) 200 Antibodies

General

Antigen Erythropoietin (EPO) Antibodies
Epitope AA 1-166
(21), (18), (16), (13), (13), (10), (8), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(284), (52), (31), (5), (2), (1), (1)
Host Rabbit
(166), (139), (4), (3), (3), (1)
Clonality
Polyclonal
Conjugate This EPO antibody is un-conjugated
(25), (19), (14), (8), (6), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(217), (207), (21), (18), (11), (11), (10), (8), (6), (6), (6), (4), (3), (3), (3), (2), (2), (1), (1)
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Product Details anti-EPO Antibody

Target Details EPO Application Details Handling Images
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Erythropoietin(EPO) detection. Tested with WB, IHC-P, ELISA, IP in Human.
Gene Name: erythropoietin
Protein Name: Erythropoietin
Purification Immunogen affinity purified.
Immunogen E. coli-derived human EPO recombinant protein(Position: A1-R166).
Isotype IgG

Target Details EPO

Product Details anti-EPO Antibody Application Details Handling Images back to top
Antigen
Alternative Name EPO (EPO Antibody Abstract)
Background EPO, Erythropoietin, is an acidic glycoprotein hormone with a molecular mass of 34 kD. It is a cytokine for erythrocyte(red blood cell) precursors in the bone marrow. This gene is mapped to 7q22.1. It is produced by interstitial fibroblasts in the kidney in close association with peritubular capillary and tubular epithelial cells. It is also produced in perisinusoidal cells in the liver. While liver production predominates in the fetal and perinatal period, renal production is predominant during adulthood. As the prime regulator of red cell production, its major functions are to promote erythroid differentiation and to initiate hemoglobin synthesis. It also has other known biological functions. For example, erythropoietin plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process.
UniProt P01588
Research Area Cancer, Organogenesis, Hematopoietic Progenitors, Angiogenesis
Pathways JAK-STAT Signaling

Application Details

Product Details anti-EPO Antibody Target Details EPO Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Species: Human
IP: Species: Human
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-EPO Antibody Target Details EPO Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-EPO Antibody Target Details EPO Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-EPO antibody (Erythropoietin) (AA 1-166) (ABIN3042813) Anti-human EPO antibody, Western blotting Lane 1: Recombinant human EPO Protein 10ng ...
Immunohistochemistry (IHC) image for anti-EPO antibody (Erythropoietin) (AA 1-166) (ABIN3042813) Anti-human EPO antibody, IHC(P) IHC(P): Human Placenta Tissue