anti-Human EPO antibody for Western Blotting

Recommended EPO Antibody (supplied by: Log in to see )

Antigen
Erythropoietin (EPO) Antibodies
  • EPO
  • EP
  • MVCD2
  • erythropoietin
  • EPO
  • epo
  • Epo
Epitope
Middle Region
19
15
15
14
13
13
8
6
6
5
5
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
337
75
42
7
4
2
2
2
1
1
1
1
Host
Rabbit
189
172
15
5
3
1
Clonality
Polyclonal
Conjugate
This EPO antibody is un-conjugated
28
21
16
10
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
250
216
67
60
57
52
31
13
11
11
7
6
4
3
2
2
2
2
2
1
1
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Catalog No. ABIN2781825
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN952117 EIA WB Rabbit Ig N-Term, AA 19-48 Log in to see Polyclonal 5
3.2182703 ABIN969109 ICC ELISA WB Mouse IgG1 Log in to see 4F11 1
3.2182703 ABIN3042813 ELISA IHC (p) WB Rabbit IgG AA 1-166 Log in to see Polyclonal
3.2182703 ABIN515362 ELISA WB Mouse IgG2a kappa AA 28-193, full length Log in to see 4G7 1
3.2182703 ABIN2693636 WB Rabbit Ig Fraction Log in to see Polyclonal
3.2182703 ABIN1536744 WB Rabbit Ig AA 155-184, C-Term Log in to see Polyclonal
3.2182703 ABIN2706118 WB Rabbit Center Log in to see Polyclonal
3.2182703 ABIN1107137 EIA IF WB Mouse IgG1 Log in to see 4F11 2
3.2182703 ABIN1539152 WB Rabbit Ig AA 20-48, N-Term Log in to see Polyclonal
3.2182703 ABIN1539942 WB Mouse IgM Log in to see 511CT20-2-4
3.2182703 ABIN1540016 WB Rabbit Ig Log in to see Polyclonal
3.2182703 ABIN1876864 IHC WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN4309572 ELISA ICC IF WB Mouse IgG1 Log in to see 4F11
3.2182703 ABIN1077998 ICC IHC IP WB Rabbit IgG AA 28-193 Log in to see Polyclonal
3.2182703 ABIN2467920 ELISA WB Chicken AA 1-193 Log in to see Polyclonal
3.2182703 ABIN1327292 ELISA WB Mouse IgG2a kappa AA 28-193, full length Log in to see 4G7
3.2182703 ABIN3030868 ELISA WB Mouse IgM Log in to see 511CT20-2-4
3.2182703 ABIN1172018 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN635113 WB Rabbit Middle Region Log in to see Polyclonal
3.2182703 ABIN515360 WB Mouse AA 1-193, full length Log in to see Polyclonal

General

Antigen Erythropoietin (EPO) Antibodies
Epitope Middle Region
(19), (15), (15), (14), (13), (13), (8), (6), (6), (5), (5), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(337), (75), (42), (7), (4), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(189), (172), (15), (5), (3), (1)
Clonality
Polyclonal
Conjugate This EPO antibody is un-conjugated
(28), (21), (16), (10), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(250), (216), (67), (60), (57), (52), (31), (13), (11), (11), (7), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1)
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Product Details anti-EPO Antibody

Target Details EPO Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN976390
Sequence KEAISPPDAA SAAPLRTITA DTFRKLFRVY SNFLRGKLKL YTGEACRTGD
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%
Characteristics This is a rabbit polyclonal antibody against EPO. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human EPO

Target Details EPO

Product Details anti-EPO Antibody Application Details Handling Images back to top
Antigen
Alternative Name EPO (EPO Antibody Abstract)
Background This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Alias Symbols: EP, MGC138142, MVCD2
Protein Interaction Partner: EP300, EPOR, SIM2, RB1, HIF1A,
Protein Size: 193
Molecular Weight 18 kDa
Gene ID 2056
NCBI Accession NM_000799, NP_000790
UniProt P01588
Research Area Cancer, Organogenesis, Hematopoietic Progenitors, Angiogenesis
Pathways JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity

Application Details

Product Details anti-EPO Antibody Target Details EPO Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 193 AA

Restrictions For Research Use only

Handling

Product Details anti-EPO Antibody Target Details EPO Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-EPO Antibody Target Details EPO Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Erythropoietin (EPO) (Middle Region) antibody (ABIN2781825) WB Suggested Anti-EPO Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive ...