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Mouse (Murine) EPO ELISA Kit

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Antigen
Erythropoietin (EPO) ELISA Kits
  • EP
  • EPO
  • MVCD2
Epitope
AA 27-192
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
17
16
15
9
8
6
5
4
3
3
3
2
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
46.9-3000 pg/mL
Minimum Detection Limit
46.9 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN921054
$ 430.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN415364 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 6.25-400 pg/mL 6.25 pg/mL Log in to see
0.32693866 ABIN1114810 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32693866 ABIN2708505 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.32693866 ABIN2113847 Cell Culture Supernatant, Serum, Plasma (heparin), Tissue Homogenate Log in to see
0.0012117105 ABIN366328 Sandwich ELISA Serum, Plasma, Tissue Homogenate 4.7-300 μIU/mL 4.7 μIU/mL Log in to see
0.0012117105 ABIN773070 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012117105 ABIN2684142 Sandwich ELISA Plasma (heparin), Cell Culture Supernatant, Serum, Tissue Homogenate 46.9-3000 pg/mL 46.9 pg/mL Log in to see 1
0.0012117105 ABIN1569609 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 6.25-400 pg/mL 6.25 pg/mL Log in to see
0.0012117105 ABIN2042456 Sandwich ELISA 46.9-3000 pg/mL 46.9 pg/mL Log in to see
0.0012117105 ABIN2533111 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 6.25-400 pg/mL 6.25 pg/mL Log in to see
0.0012117105 ABIN455063 78-5000 pg/mL 78 pg/mL Log in to see
0.0012117105 ABIN2081514 Sandwich ELISA Log in to see
0.0012117105 ABIN2947313 0.156-10 ng/mL 0.156 ng/mL Log in to see

Top referenced EPO ELISA Kit for ELISA

  • Mouse (Murine) EPO ELISA Kit for Sandwich ELISA - ABIN2684142 (1 Publications): Doeppner, Mlynarczuk-Bialy, Kuckelkorn, Kaltwasser, Herz, Hasan, Hermann, Bähr: The novel proteasome inhibitor BSc2118 protects against cerebral ischaemia through HIF1A accumulation and enhanced angioneurogenesis. in Brain : a journal of neurology 2012 (PubMed)

  • Mouse (Murine) EPO ELISA Kit for Sandwich ELISA - ABIN921054 (1 Publications): Sirén, Fratelli, Brines, Goemans, Casagrande, Lewczuk, Keenan, Gleiter, Pasquali, Capobianco, Mennini, Heumann, Cerami, Ehrenreich, Ghezzi: Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress. in Proceedings of the National Academy of Sciences of the United States of America 2001 (PubMed)

Similar EPO ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 14 ELISA Kits

General

Antigen Erythropoietin (EPO) ELISA Kits
Epitope AA 27-192
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(17), (16), (15), (9), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 46.9-3000 pg/mL
Minimum Detection Limit 46.9 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details EPO ELISA Kit

Target details Application Details Handling References for EPO Kit (ABIN921054) Images
Purpose For quantitative detection of mouse EPO in cell culture supernates, serum, plasma(heparin) and tissue homogenates.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Tissue Homogenate
Detection Method Colorimetric
Specificity Natural and recombinant mouse EPO
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <15pg/mL
Components
  • 96-well plate precoated with anti- mouse EPO antibod - 1
  • Lyophilized recombinant mouse EPO standar - 10ng/tubex2
  • Biotinylated anti- mouse EPO antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A27-R192

Target details

Product Details EPO ELISA Kit Application Details Handling References for EPO Kit (ABIN921054) Images back to top
Antigen
Alternative Name EPO (EPO ELISA Kit Abstract)
Background Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte(red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.
Gene ID 13856
UniProt P07321
Research Area Angiogenesis, Cancer, Hematopoietic Progenitors, Organogenesis
Pathways JAK-STAT Signaling

Application Details

Product Details EPO ELISA Kit Target details Handling References for EPO Kit (ABIN921054) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse EPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for EPO has been precoated onto 96-well plates. Standards(NSO, A27-R192)and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse EPO amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin) or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse EPO standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 225, Standard deviation: 10.13, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 457, Standard deviation: 17.4, CV(%): 3.8
  • Sample 3: n=16, Mean(pg/ml): 1046, Standard deviation: 55.4, CV(%): 5.3,
  • Sample 1: n=24, Mean(pg/ml): 246, Standard deviation: 23.12, CV(%): 9.4
  • Sample 2: n=24, Mean(pg/ml): 486, Standard deviation: 36.45, CV(%): 7.5
  • Sample 3: n=24, Mean(pg/ml): 1118, Standard deviation: 43.6, CV(%): 3.9
Restrictions For Research Use only

Handling

Product Details EPO ELISA Kit Target details Application Details References for EPO Kit (ABIN921054) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for EPO Kit (ABIN921054)

Product Details EPO ELISA Kit Target details Application Details Handling Images back to top
Background publications

Sirén, Fratelli, Brines, Goemans, Casagrande, Lewczuk, Keenan, Gleiter, Pasquali, Capobianco, Mennini, Heumann, Cerami, Ehrenreich, Ghezzi: "Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 7, pp. 4044-9, 2001

Images

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Supplier Images
ELISA image for EPO ELISA Kit (Erythropoietin) (ABIN921054) Mouse EPO PicoKine ELISA Kit standard curve