Mouse (Murine) EPO ELISA Kit

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Antigen
Erythropoietin (EPO) ELISA Kits
  • EPO
  • EP
  • MVCD2
  • erythropoietin
  • EPO
  • epo
  • Epo
Epitope
AA 27-192
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
23
22
22
13
10
9
6
6
5
4
4
3
3
2
1
1
Method Type
Sandwich ELISA
Detection Range
46.9-3000 pg/mL
Minimum Detection Limit
46.9 pg/mL
Application
ELISA
Options
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Catalog No. ABIN921054
$ 430.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.090268 ABIN415364 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 6.25-400 pg/mL 6.25 pg/mL Log in to see
10.090268 ABIN366328 Sandwich ELISA Serum, Plasma, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
10.090268 ABIN1114810 Sandwich ELISA Serum, Plasma, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see
10.090268 ABIN491741 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 1.37-1.000 pg/mL 1.37 pg/mL Log in to see
10.090268 ABIN4885513 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 1.5-400 pg/mL 1.5 pg/mL Log in to see
10.090268 ABIN2708505 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 46.9-3000 pg/mL 46.9 pg/mL Log in to see
10.090268 ABIN2113847 Cell Culture Supernatant, Serum, Plasma (heparin), Tissue Homogenate Log in to see
1 ABIN1569609 Sandwich ELISA Biological Fluids, Cell Lysate, Plasma, Serum, Tissue Homogenate 6.25-400 pg/mL 6.25 pg/mL Log in to see
1 ABIN773070 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
1 ABIN4947538 Sandwich ELISA Log in to see
1 ABIN5653973 Sandwich ELISA Biological Fluids, Cell Lysate, Plasma, Serum, Tissue Homogenate 6.25 pg/mL - 400 pg/mL 6.25 pg/mL Log in to see
1 ABIN5653972 Sandwich ELISA Biological Fluids, Cell Lysate, Plasma, Serum, Tissue Homogenate 1.37 pg/mL - 1000 pg/mL 1.37 pg/mL Log in to see
1 ABIN5593376 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN2684142 Sandwich ELISA Plasma (heparin), Cell Culture Supernatant, Serum, Tissue Homogenate 46.9-3000 pg/mL 46.9 pg/mL Log in to see 1
1 ABIN5522730 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 47-3000 pg/mL 47 pg/mL Log in to see
1 ABIN2042456 Sandwich ELISA 46.9-3000 pg/mL 46.9 pg/mL Log in to see
1 ABIN2533111 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 6.25-400 pg/mL 6.25 pg/mL Log in to see
1 ABIN4968934 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 6.25-400 pg/mL 6.25 pg/mL Log in to see
1 ABIN455063 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN2947313 0.156-10 ng/mL 0.156 ng/mL Log in to see

General

Antigen Erythropoietin (EPO) ELISA Kits
Epitope AA 27-192
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(23), (22), (22), (13), (10), (9), (6), (6), (5), (4), (4), (3), (3), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 46.9-3000 pg/mL
Minimum Detection Limit 46.9 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details EPO ELISA Kit

Target details Application Details Handling References for EPO Kit (ABIN921054) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse EPO
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: NSO
Immunogen sequence: A27-R192
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <15pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A27-R192

Target details

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Antigen
Alternative Name EPO (EPO ELISA Kit Abstract)
Background

Protein Function: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Background: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte(red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.

Synonyms: Erythropoietin,Epo,

Full Gene Name: Erythropoietin

Cellular Localisation: Secreted.
Gene ID 13856
UniProt P07321
Research Area Angiogenesis, Cancer, Hematopoietic Progenitors, Organogenesis
Pathways JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Tissue Specificity: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Plate Pre-coated
Protocol mouse EPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for EPO has been precoated onto 96-well plates. Standards(NSO, A27-R192)and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse EPO amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin) or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse EPO standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 225, Standard deviation: 10.13, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 457, Standard deviation: 17.4, CV(%): 3.8
  • Sample 3: n=16, Mean(pg/ml): 1046, Standard deviation: 55.4, CV(%): 5.3,
  • Sample 1: n=24, Mean(pg/ml): 246, Standard deviation: 23.12, CV(%): 9.4
  • Sample 2: n=24, Mean(pg/ml): 486, Standard deviation: 36.45, CV(%): 7.5
  • Sample 3: n=24, Mean(pg/ml): 1118, Standard deviation: 43.6, CV(%): 3.9
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for EPO Kit (ABIN921054)

Product Details EPO ELISA Kit Target details Application Details Handling Images back to top
Background publications

Sirén, Fratelli, Brines, Goemans, Casagrande, Lewczuk, Keenan, Gleiter, Pasquali, Capobianco, Mennini, Heumann, Cerami, Ehrenreich, Ghezzi: "Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 7, pp. 4044-9, 2001

Images

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Supplier Images
ELISA image for Erythropoietin (EPO) ELISA Kit (ABIN921054) Mouse EPO PicoKine ELISA Kit standard curve