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anti-Human EPOR antibody for Cell-ELISA

Recommended EPOR Antibody (supplied by: Log in to see )

Antigen
Erythropoietin Receptor (EPOR) Antibodies
  • EPO-R
  • epor
  • EPOR
Reactivity
Human
143
50
45
3
1
1
Host
Mouse
112
31
13
Clonality
Monoclonal
Conjugate
This EPOR antibody is un-conjugated
10
7
4
4
4
3
1
1
1
1
1
1
1
1
1
1
Application
Cell-ELISA (cELISA), Flow Cytometry (FACS)
110
78
26
23
15
11
11
9
8
4
2
1
1
Supplier
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Catalog No. ABIN1720915
$ 358.05
Plus shipping costs $45.00

Top referenced anti-EPOR antibody for Cell-ELISA

Similar anti-EPOR Antibodies

Application / Reactivity Human
Cell-ELISA (cELISA) 1 Antibodies
ELISA 71 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 24 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 12 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 105 Antibodies

General

Antigen Erythropoietin Receptor (EPOR) Antibodies
Reactivity Human
(143), (50), (45), (3), (1), (1)
Host Mouse
(112), (31), (13)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EPOR antibody is un-conjugated
(10), (7), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cell-ELISA (cELISA), Flow Cytometry (FACS)
(110), (78), (26), (23), (15), (11), (11), (9), (8), (4), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-EPOR Antibody

Target Details EPOR Application Details Handling References for anti-EPOR Antibody (ABIN1720915) Images
Specificity Anti-human Epo-R
Characteristics Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells
Purification Protein G
Immunogen genetic immunisation with cDNA encoding the ECD of human Epo-R
Clone VP-2E8
Isotype IgG1

Target Details EPOR

Product Details anti-EPOR Antibody Application Details Handling References for anti-EPOR Antibody (ABIN1720915) Images back to top
Antigen
Alternative Name EPOR (EPOR Antibody Abstract)
Background Erythropoietin receptor (Epo-R) belongs to the cytokine receptor family and is a 507 amino acid type I transmembrane protein. EpoR pre-exists as dimers which changes the homodimerized state after binding of its 34 kDa ligand erythropoietin (Epo). Erythropoietin is the primary regulator of erythropoiesis, and promotes the survival, proliferation, and differentiation of erythroid progenitor cells. Both, Epo and Epo-R are essential for the production of red blood cells due to Epo exerts its function through the Epo receptor. The Epo-R is also expressed in many organs outside the bone marrow, suggesting that Epo is a pleiotropic anti-apoptotic factor. Signaling pathways have been shown to influence numerous cellular functions in normal and tumor cells, including proliferation, apoptosis, and drug resistance.
UniProt P19235
Pathways JAK-STAT Signaling

Application Details

Product Details anti-EPOR Antibody Target Details EPOR Handling References for anti-EPOR Antibody (ABIN1720915) Images back to top
Application Notes Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: Erythropoietin receptor

Restrictions For Research Use only

Handling

Product Details anti-EPOR Antibody Target Details EPOR Application Details References for anti-EPOR Antibody (ABIN1720915) Images back to top
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C

References for anti-EPOR Antibody (ABIN1720915)

Product Details anti-EPOR Antibody Target Details EPOR Application Details Handling Images back to top
Product cited in:

Maxwell, Melendez-Rodríguez, Matchett, Aragones, Ben-Califa, Jaekel, Hengst, Lindner, Bernardini, Brockmeier, Fandrey, Grunert, Oster, Mittelman, El-Tanani, Thiersch, Schneider Gasser, Gassmann et al.: "Novel antibodies directed against the human erythropoietin receptor: creating a basis for clinical implementation. ..." in: British journal of haematology, Vol. 168, Issue 3, pp. 429-42, 2015

Hedley, Allan, Xenocostas: "The role of erythropoietin and erythropoiesis-stimulating agents in tumor progression." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 20, pp. 6373-80, 2011

Background publications

Wilson, Jolliffe: "The structure, organization, activation and plasticity of the erythropoietin receptor." in: Current opinion in structural biology, Vol. 9, Issue 6, pp. 696-704, 2000

Livnah, Stura, Middleton, Johnson, Jolliffe, Wilson: "Crystallographic evidence for preformed dimers of erythropoietin receptor before ligand activation." in: Science (New York, N.Y.), Vol. 283, Issue 5404, pp. 987-90, 1999

Winkelmann: "The human erythropoietin receptor." in: International journal of cell cloning, Vol. 10, Issue 5, pp. 254-61, 1993

Images

Product Details anti-EPOR Antibody Target Details EPOR Application Details Handling References for anti-EPOR Antibody (ABIN1720915) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EPOR antibody (Erythropoietin Receptor) (ABIN1720915) BOSC23 cells were transiently transfected with an expression vector encoding either E...
SDS-PAGE (SDS) image for anti-EPOR antibody (Erythropoietin Receptor) (ABIN1720915) CGE analysis of purified VP-2E8 monoclonal antibody. Lane 1: molecular weight marker,...
Western Blotting (WB) image for anti-EPOR antibody (Erythropoietin Receptor) (ABIN1720915) Immunoprecipitation of EpoR with and from A549 lung carcinoma cells expressing contro...