anti-Mouse (Murine) FYN antibody for Immunocytochemistry

Recommended FYN Antibody (supplied by: Log in to see )

Antigen
FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
  • im:6908851
  • si:dkey-119m7.5
  • zgc:109996
  • c-fyn
  • cfyn-a
  • SLK
  • SYN
  • p59-FYN
  • PKC
  • fyn
  • zgc:86720
  • AI448320
  • AW552119
  • FYN oncogene related to SRC, FGR, YES
  • FYN oncogene related to SRC, FGR, YES b
  • c-fyn protein
  • FYN oncogene related to SRC, FGR, YES a
  • Fyn proto-oncogene
  • FYN
  • fyn
  • fynb
  • Fyn
  • fyna
Reactivity
Human, Mouse (Murine)
250
156
56
10
9
8
5
5
4
4
4
3
2
1
1
Host
Mouse
138
114
19
Clonality
Monoclonal
Conjugate
This FYN antibody is un-conjugated
9
9
9
8
7
7
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
250
121
64
50
33
28
26
23
5
4
4
2
2
2
2
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Catalog No. ABIN269149
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN94311 ICC IHC (p) IP WB Mouse IgG2b AA 7-176 Log in to see FYN-01 10
1 ABIN239824 ICC IHC (p) IP WB Mouse IgG AA 7-176 Log in to see FYN-01
1 ABIN2469997 ICC IP IHC WB Mouse IgG AA 7-176 Log in to see FYN-01
1 ABIN442044 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN4903712 ICC WB Mouse IgG Log in to see
1 ABIN1723376 ICC FACS IHC (p) IP WB Mouse IgG AA 7-176 Log in to see FYN-01
1 ABIN3200170 ICC IHC (p) IP WB Mouse IgG2b Log in to see FYN-01
1 ABIN4312917 ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG2b Log in to see FYN-01
1 ABIN4312918 ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG2b Log in to see FYN-01
1 ABIN4312922 ICC IF IHC IHC (p) WB DyLight 755 Mouse IgG2b Log in to see FYN-01
1 ABIN4312924 ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG2b Log in to see FYN-01
1 ABIN4312926 ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG2b Log in to see FYN-01
1 ABIN4312928 ICC IF IHC IHC (p) WB Alexa Fluor 647 Mouse IgG2b Log in to see FYN-01
1 ABIN4312920 ICC IF IHC IHC (p) IP WB PerCP Mouse IgG2b Log in to see FYN-01
1 ABIN4312927 ICC IF IHC IHC (p) WB Alexa Fluor 488 Mouse IgG2b Log in to see FYN-01
1 ABIN4312929 ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG2b Log in to see FYN-01
1 ABIN4312923 ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG2b Log in to see FYN-01
1 ABIN4312921 ICC IF IHC IHC (p) IP WB Allophycocyanin Mouse IgG2b Log in to see FYN-01
1 ABIN4312919 ICC IF IHC IHC (p) IP WB PE Mouse IgG2b Log in to see FYN-01

General

Antigen FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
Reactivity Human, Mouse (Murine)
(250), (156), (56), (10), (9), (8), (5), (5), (4), (4), (4), (3), (2), (1), (1)
Host Mouse
(138), (114), (19)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FYN antibody is un-conjugated
(9), (9), (9), (8), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(250), (121), (64), (50), (33), (28), (26), (23), (5), (4), (4), (2), (2), (2), (2)
Pubmed 4 references available
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Product Details anti-FYN Antibody

Target Details FYN Application Details Handling References for anti-FYN antibody (ABIN269149) Images
Specificity The reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (59 kDa),which belongs to the Src family of cytoplasmic tyrosine kinases.
Purification Protein A purified
Immunogen Bacterial expressed recombinant fragment, corresponding to amino acids 7-176 of Human Fyn.
Clone FYN-01
Isotype IgG2b

Target Details FYN

Product Details anti-FYN Antibody Application Details Handling References for anti-FYN antibody (ABIN269149) Images back to top
Antigen
Alternative Name Fyn (FYN Antibody Abstract)
Background Gene Symbol: FYN
Gene ID 2534
Research Area Signaling, Alzheimer's Disease, Cancer, Tyrosine Kinases
Pathways JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-FYN Antibody Target Details FYN Handling References for anti-FYN antibody (ABIN269149) Images back to top
Application Notes Western Blot 2 μg/mL, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:50, Immunohistochemistry-Paraffin 5 μg/mLPredicted molecular weight: 59 kDa. Purity is > 95 % (by SDS-PAGE). Immunoprecipitation - Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1 % laurylmaltosid (w/v), 50 mM NaF) Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-FYN Antibody Target Details FYN Application Details References for anti-FYN antibody (ABIN269149) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-FYN antibody (ABIN269149)

Product Details anti-FYN Antibody Target Details FYN Application Details Handling Images back to top
Product cited in:

Grossman, Har-Paz, Gindi, Levi, Miller, Nevo, Galiani, Dekel, Shalgi: "Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments." in: Scientific reports, Vol. 7, Issue 1, pp. 2238, 2017 Method employed by authors: Western Blotting (WB)

Hu, Takano, Xiang, Gilkes, Luo, Semenza: "Hypoxia-inducible factors enhance glutamate signaling in cancer cells." in: Oncotarget, Vol. 5, Issue 19, pp. 8853-68, 2014 (Sample species: Human). Further details: Western Blotting

Taniguchi, Xia, Goldberg, Lee, Shah, Stavar, Masson, Momen, Shikatani, John, Husain, Fantus: "Inhibition of Src kinase blocks high glucose-induced EGFR transactivation and collagen synthesis in mesangial cells and prevents diabetic nephropathy in mice." in: Diabetes, Vol. 62, Issue 11, pp. 3874-86, 2013

Jia, Pan, Ding, Rong, Liu, Geng, Qiao, Liang: "The N-terminal region of IFITM3 modulates its antiviral activity by regulating IFITM3 cellular localization." in: Journal of virology, Vol. 86, Issue 24, pp. 13697-707, 2012 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Images

Product Details anti-FYN Antibody Target Details FYN Application Details Handling References for anti-FYN antibody (ABIN269149) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) antibody (ABIN269149) Immunoprecipitation: Fyn Antibody (FYN-01) [ABIN269149] - Immunoprecipitation of Fyn ...
Immunofluorescence (IF) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) antibody (ABIN269149) Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [ABIN269149] - Staining...
Immunofluorescence (IF) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) antibody (ABIN269149) Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [ABIN269149] - Staining...
Immunofluorescence (IF) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) antibody (ABIN269149) Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [ABIN269149] - Staining...