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anti-Mouse (Murine) FYN antibody for Immunoprecipitation

Recommended FYN Antibody (supplied by: Log in to see )

Antigen
FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
  • AI448320
  • AW552119
  • c-fyn
  • cfyn-a
  • fyn
  • im:6908851
  • p59-FYN
  • PKC
  • si:dkey-119m7.5
  • SLK
  • SYN
  • zgc:86720
  • zgc:109996
Epitope
AA 7-176
56
33
20
20
15
8
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
223
124
39
7
7
7
2
1
1
1
1
Host
Mouse
132
99
16
Clonality
Monoclonal
Conjugate
This FYN antibody is un-conjugated
9
9
8
8
7
7
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
227
114
66
39
39
29
28
19
6
4
2
2
2
2
1
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Catalog No. ABIN94311
$ 288.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN256460 IP WB Mouse IgG AA 7-176 Log in to see FYN-59
0.00089556724 ABIN120706 IF IHC (p) IP WB Mouse IgG2b AA 7-176 Log in to see FYN-01
0.00089556724 ABIN2469756 IP IHC WB Mouse IgG AA 7-176 Log in to see FYN-59
0.00089556724 ABIN2469997 ICC IP IHC WB Mouse IgG AA 7-176 Log in to see FYN-01
0.00089556724 ABIN2704810 IP IHC WB Rabbit C-Term, pTyr530 Log in to see Polyclonal
0.00089556724 ABIN269149 ICC IF IHC (p) IP IHC WB Mouse IgG2b Log in to see FYN-01 3
0.00089556724 ABIN1723376 ICC FACS IHC (p) IP WB Mouse IgG Log in to see FYN-01
0.00089556724 ABIN1724003 IHC (p) IP WB Mouse IgG Log in to see FYN-59
0.00089556724 ABIN2473714 IHC (fro) IP WB Mouse IgG2b AA 7-176 Log in to see FYN-01 4
0.00089556724 ABIN201639 IHC (fro) IP ELISA WB Mouse IgG1 AA 1-206 Log in to see
0.00089556724 ABIN4312917 ICC IF IHC IHC (p) IP WB DyLight 350 Mouse IgG2b Log in to see FYN-01
0.00089556724 ABIN4312918 ICC IF IHC IHC (p) IP WB DyLight 405 Mouse IgG2b Log in to see FYN-01
0.00089556724 ABIN4312920 ICC IF IHC IHC (p) IP WB PerCP Mouse IgG2b Log in to see FYN-01
0.00089556724 ABIN4312921 ICC IF IHC IHC (p) IP WB Allophycocyanin Mouse IgG2b Log in to see FYN-01
0.00089556724 ABIN4312923 ICC IF IHC IHC (p) IP WB DyLight 680 Mouse IgG2b Log in to see FYN-01
0.00089556724 ABIN4312924 ICC IF IHC IHC (p) IP WB DyLight 488 Mouse IgG2b Log in to see FYN-01
0.00089556724 ABIN4312925 ICC IF IHC IHC (p) IP WB DyLight 405LS Mouse IgG2b Log in to see FYN-01
0.00089556724 ABIN4312926 ICC IF IHC IHC (p) IP WB Alexa Fluor 405 Mouse IgG2b Log in to see FYN-01
0.00089556724 ABIN4312928 ICC IF IHC IHC (p) IP WB Alexa Fluor 647 Mouse IgG2b Log in to see FYN-01
0.00089556724 ABIN4312929 ICC IF IHC IHC (p) IP WB Alexa Fluor 700 Mouse IgG2b Log in to see FYN-01

Top referenced anti-FYN antibody for Immunoprecipitation

Similar anti-FYN Antibodies

Application / Reactivity Mouse (Murine) Human
Biochemical Assay (BCA) 2 Antibodies 2 Antibodies
ELISA 45 Antibodies 99 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies 19 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Western Blotting (WB) 112 Antibodies

General

Antigen FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
Epitope AA 7-176
(56), (33), (20), (20), (15), (8), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(223), (124), (39), (7), (7), (7), (2), (1), (1), (1), (1)
Host Mouse
(132), (99), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FYN antibody is un-conjugated
(9), (9), (8), (8), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(227), (114), (66), (39), (39), (29), (28), (19), (6), (4), (2), (2), (2), (2), (1)
Pubmed 10 references available
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Product Details anti-FYN Antibody

Target Details FYN Application Details Handling References for anti-FYN Antibody (ABIN94311) Images
Specificity The antibody FYN-01 reacts with Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase.
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Bacterially expressed recombinant fragment of human Fyn (aa 7-176).
Clone FYN-01
Isotype IgG2b

Target Details FYN

Product Details anti-FYN Antibody Application Details Handling References for anti-FYN Antibody (ABIN94311) Images back to top
Antigen
Alternative Name FYN (FYN Antibody Abstract)
Background Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.
Pathways JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-FYN Antibody Target Details FYN Handling References for anti-FYN Antibody (ABIN94311) Images back to top
Application Notes Immunoprecipitation:
Positive control: J77 T cell line, Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1 % laurylmaltosid (w/v), 50 mM NaF)
Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.
Western Blotting: Recommended dilution: 2 µg/mL
Positive control: JURKAT human T cell leukemia cell line
Immunohistochemistry (paraffin sections) Recommended dilution: 5 µg/mL
Positive tissue: brain

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-FYN Antibody Target Details FYN Application Details References for anti-FYN Antibody (ABIN94311) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-FYN Antibody (ABIN94311)

Product Details anti-FYN Antibody Target Details FYN Application Details Handling Images back to top
Product cited in:

Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, 2007

Background publications

Rivera, Olivera: "A current understanding of Fc epsilon RI-dependent mast cell activation." in: Current allergy and asthma reports, Vol. 8, Issue 1, pp. 14-20, 2008

Makinodan, Manabe, Makinodan, Yamauchi, Matsuyoshi, Sakumura, Tatsumi, Wanaka: "A novel role for Fyn: change in sphere formation ability in murine embryonic stem cells." in: Neuroscience, Vol. 147, Issue 1, pp. 1-4, 2007

Samayawardhena, Kapur, Craig: "Involvement of Fyn kinase in Kit and integrin-mediated Rac activation, cytoskeletal reorganization, and chemotaxis of mast cells." in: Blood, Vol. 109, Issue 9, pp. 3679-86, 2007

Filby, Seddon, Kleczkowska, Salmond, Tomlinson, Smida, Lindquist, Schraven, Zamoyska: "Fyn regulates the duration of TCR engagement needed for commitment to effector function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 7, pp. 4635-44, 2007

Wu, Hsu, Gleichman, Baconguis, Coulter, Lynch: "Fyn-mediated phosphorylation of NR2B Tyr-1336 controls calpain-mediated NR2B cleavage in neurons and heterologous systems." in: The Journal of biological chemistry, Vol. 282, Issue 28, pp. 20075-87, 2007

Hattori, Uchino, Isosaka, Maekawa, Iyo, Sato, Kohsaka, Yagi, Yuasa: "Fyn is required for haloperidol-induced catalepsy in mice." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7129-35, 2006

Talmor-Cohen, Tomashov-Matar, Tsai, Kinsey, Shalgi: "Fyn kinase-tubulin interaction during meiosis of rat eggs." in: Reproduction (Cambridge, England), Vol. 128, Issue 4, pp. 387-93, 2004

Utting, Priatel, Teh, Teh: "p59fyn (Fyn) promotes the survival of anergic CD4-CD8- alpha beta TCR+ cells but negatively regulates their proliferative response to antigen stimulation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 3, pp. 1540-6, 2001

Images

Product Details anti-FYN Antibody Target Details FYN Application Details Handling References for anti-FYN Antibody (ABIN94311) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-FYN antibody (FYN Oncogene Related To SRC, FGR, YES) (AA 7-176) (ABIN94311) Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats...
Immunofluorescence (IF) image for anti-FYN antibody (FYN Oncogene Related To SRC, FGR, YES) (AA 7-176) (ABIN94311) Immunofluorescence staining of Fyn in human primary fibroblasts using anti-Fyn (; gre...
Immunofluorescence (IF) image for anti-FYN antibody (FYN Oncogene Related To SRC, FGR, YES) (AA 7-176) (ABIN94311) Immunofluorescence staining of Fyn in human HeLa cell line using anti-Fyn
Immunofluorescence (IF) image for anti-FYN antibody (FYN Oncogene Related To SRC, FGR, YES) (AA 7-176) (ABIN94311) Immunofluorescence staining of Fyn in murine transformed fibroblasts using anti-Fyn (...