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anti-Human IFNAR2 antibody for ELISA

Recommended IFNAR2 Antibody (supplied by: Log in to see )

Antigen
Interferon alpha/beta Receptor 2 (IFNAR2) Antibodies
  • AI747302
  • IFN-alpha-REC
  • IFN-R
  • IFN-R-2
  • IFNABR
  • Ifnar-2
  • IFNARB
  • IFNBR
Epitope
AA 1-243
15
4
4
3
2
1
1
1
1
Reactivity
Human
54
5
1
Host
Mouse
25
20
12
Clonality
Monoclonal
Conjugate
This IFNAR2 antibody is un-conjugated
6
3
2
1
1
1
Minimum Detection Limit
0.039 ng/well
Application
Flow Cytometry (FACS), ELISA
27
22
12
11
6
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN1996557
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.001220287 ABIN462291 IHC (p) ELISA Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN1996565 ELISA Rabbit IgG AA 1-243 Log in to see Polyclonal 2
0.001220287 ABIN1996563 ELISA Rabbit IgG AA 1-243 Log in to see Polyclonal 2
0.001220287 ABIN207561 ELISA WB Rabbit C-Term Log in to see Polyclonal
0.001220287 ABIN335143 ELISA WB Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN2466702 ELISA Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN1735773 IHC (p) ELISA Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN2288084 ELISA Alkaline Phosphatase (AP) Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN2288086 ELISA APC Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN2288088 ELISA Biotin Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN2288090 ELISA FITC Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN2288092 ELISA PE Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN2288080 IHC ELISA Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN2288082 ELISA Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN1087538 ELISA WB Rabbit IgG Log in to see Polyclonal
0.001220287 ABIN1087537 ELISA WB Rabbit IgG Log in to see Polyclonal
0.001220287 ABIN2563347 ELISA Goat IgG Internal Region Log in to see Polyclonal
0.001220287 ABIN2288101 ELISA HRP Goat Internal Region Log in to see Polyclonal
0.001220287 ABIN2143900 ELISA WB Rabbit IgG Log in to see Polyclonal
0.001220287 ABIN2419327 ELISA WB Rabbit IgG Log in to see Polyclonal

Similar anti-IFNAR2 Antibodies

Application / Reactivity Human
ELISA 28 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 23 Antibodies

General

Antigen Interferon alpha/beta Receptor 2 (IFNAR2) Antibodies
Epitope AA 1-243
(15), (4), (4), (3), (2), (1), (1), (1), (1)
Reactivity Human
(54), (5), (1)
Host Mouse
(25), (20), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IFNAR2 antibody is un-conjugated
(6), (3), (2), (1), (1), (1)
Minimum Detection Limit 0.039 ng/well
Application Flow Cytometry (FACS), ELISA
(27), (22), (12), (11), (6), (2), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IFNAR2 Antibody

Target Details IFNAR2 Application Details Handling References for anti-IFNAR2 Antibody (ABIN1996557) Images
Specificity Human IFNAR2
Cross-Reactivity (Details) No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IFNAR2 (rh IFNAR2, NP_997468.1, Met 1-Lys 243). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Sterility 0.2 μm filtered
Clone 7
Isotype IgG1

Target Details IFNAR2

Product Details anti-IFNAR2 Antibody Application Details Handling References for anti-IFNAR2 Antibody (ABIN1996557) Images back to top
Antigen
Alternative Name IFNAR2 (IFNAR2 Antibody Abstract)
Background Interferon-alpha/beta receptor beta chain (IFNAR2) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Initial cell-surface IFNAR2 expression at diagnosis assessed by flow cytometry widely distributed but showed overall significantly higher expression in CML patients when compared with normal controls. In 15 fresh patients who subsequently received IFNα therapy, IFNAR2 expression at diagnosis was significantly higher in cytogenetic good responders than in poor responders. Down-regulation of IFNAR2 expression during IFNα therapy was observed only in good responders but not in poor responders. The encoded protein also functions as an antiviral factor. IFNAR2 may associate with IFNAR1 to form the type I interferon receptor. This protein serves as a receptor for interferons alpha and beta. IFNAR2 is also involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity. Following binding of IFNα2, IFNAR2 is internalized, but, instead of being routed towards degradation as it is when complexed to IFNβ, it recycles back to the cell surface.
NCBI Accession NP_997468.1
Research Area Cancer, Cytokines, Innate Immunity
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IFNAR2 Antibody Target Details IFNAR2 Handling References for anti-IFNAR2 Antibody (ABIN1996557) Images back to top
Application Notes ELISA ELISA: 0.5-1 μg/mL This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human IFNaR2. The detection limit for Human IFNaR2 is approximately 0.039 ng/well.
Restrictions For Research Use only

Handling

Product Details anti-IFNAR2 Antibody Target Details IFNAR2 Application Details References for anti-IFNAR2 Antibody (ABIN1996557) Images back to top
Buffer PBS with 5% trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C/-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -70°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Expiry Date 12 months

References for anti-IFNAR2 Antibody (ABIN1996557)

Product Details anti-IFNAR2 Antibody Target Details IFNAR2 Application Details Handling Images back to top
Background publications

Saleh, Fang, Arch, Neupane, El Fiky, Krolewski: "Regulated proteolysis of the IFNaR2 subunit of the interferon-alpha receptor." in: Oncogene, Vol. 23, Issue 42, pp. 7076-86, 2004

Saleh, Fang, Arch, Neupane, El Fiky, Krolewski: "Regulated proteolysis of the IFNaR2 subunit of the interferon-alpha receptor." in: Oncogene, Vol. 23, Issue 42, pp. 7076-86, 2004

Zinder, Boeke: "The filamentous phage (Ff) as vectors for recombinant DNA--a review." in: Gene, Vol. 19, Issue 1, pp. 1-10, 1983

Images

Product Details anti-IFNAR2 Antibody Target Details IFNAR2 Application Details Handling References for anti-IFNAR2 Antibody (ABIN1996557) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IFNAR2 antibody (Interferon alpha/beta Receptor 2) (AA 1-243) (ABIN1996557) anti-Interferon alpha/beta Receptor 2 (IFNAR2) (AA 1-243) antibody