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anti-Mouse (Murine) IFNAR2 antibody for Flow Cytometry

Recommended IFNAR2 Antibody (supplied by: Log in to see )

Antigen
Interferon alpha/beta Receptor 2 (IFNAR2) Antibodies
  • AI747302
  • IFN-alpha-REC
  • IFN-R
  • IFN-R-2
  • IFNABR
  • Ifnar-2
  • IFNARB
  • IFNBR
Reactivity
Mouse (Murine)
55
4
1
Host
Goat
25
19
13
Clonality
Polyclonal
Conjugate
This IFNAR2 antibody is conjugated to FITC
6
2
2
1
1
1
Application
Flow Cytometry (FACS)
28
22
12
11
6
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN796985
$ 742.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010482287 ABIN796986 FACS PE Goat IgG Log in to see Polyclonal

Similar anti-IFNAR2 Antibodies

Application / Reactivity Mouse (Murine)
Flow Cytometry (FACS) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Western Blotting (WB) 3 Antibodies

General

Antigen Interferon alpha/beta Receptor 2 (IFNAR2) Antibodies
Reactivity Mouse (Murine)
(55), (4), (1)
Host Goat
(25), (19), (13)
Clonality
Polyclonal
Conjugate This IFNAR2 antibody is conjugated to FITC
(6), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(28), (22), (12), (11), (6), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-IFNAR2 Antibody

Target Details IFNAR2 Application Details Handling Images
Specificity Mouse IFNA/B R2.
Purification Immunoaffinity Chromatography
Immunogen Recombinant corresponding to IFNA/B R2 from mouse Interferon expressed in NSO cells
Isotype IgG

Target Details IFNAR2

Product Details anti-IFNAR2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IFNAR2 (IFNAR2 Antibody Abstract)
Background Standard Gene Symbol: IFNAR2, Gene Family: Interferon Receptor, Synonyms: IFNAR2, IFN-alpha/beta receptor 2, IFN-alpha-REC, IFN-alpha binding protein, IFN-R, IFN-R-2, IFNARB, Type I interferon receptor 2, IFNABR
Gene ID 3455
UniProt P48551
Research Area Cancer, Cytokines, Innate Immunity
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IFNAR2 Antibody Target Details IFNAR2 Handling Images back to top
Application Notes Flo
Assay Procedure Flow Cytometry Validation
This antibody has been tested for flow cytometry using A20 cells.
1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at room temperature. Do not wash excess blocking IgG from this reaction.
2. After blocking, 10ul of conjugated antibody was added to 1 - 2.5 x 10e5 cells and incubated for 30 minutes at room temperature.
3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer.
4. The cells were resuspended in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with CFS-labeled goat IgG antibody. This procedure may need to be modified, depending upon the cell type and final utilization. Individual users may need to titrate to determine the optimal reagent amount for their specific use.
5. Cell surface expression of IFN-a/b R2 was determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 515 - 545 nm.
Restrictions For Research Use only

Handling

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Buffer PBS, up to 0.5% BSA and 0.1% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles and prolonged exposure to light.
Storage 4 °C/-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles and prolonged exposure to light.

Images

Product Details anti-IFNAR2 Antibody Target Details IFNAR2 Application Details Handling back to top
Supplier Images
 image for anti-IFNAR2 antibody (Interferon alpha/beta Receptor 2)  (FITC) (ABIN796985) Flow Cytometric analysis of A20 cells stained with ABIN796985 (filled histogram) or i...