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Mouse (Murine) IL-13 ELISA Kit

Recommended IL-13 Product (supplied by: Log in to see )

Antigen
Interleukin 13 (IL13) ELISA Kits
  • Il-13
  • IL-13
  • IL13
  • L13
  • P600
Epitope
AA 26-131
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
52
29
21
10
7
7
6
5
4
4
3
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN411312
$ 430.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.3092964 ABIN2472095 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.3092964 ABIN415504 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see 1
0.3092964 ABIN924812 Sandwich ELISA Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.3092964 ABIN1115614 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.3092964 ABIN2708539 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.3092964 ABIN2708540 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.3092964 ABIN2114266 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
0.001212079 ABIN365244 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.001212079 ABIN3032812 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 2.7-2000 pg/mL 2.7 pg/mL Log in to see
0.001212079 ABIN625137 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant Log in to see 3
0.001212079 ABIN628004 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.001212079 ABIN1570618 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.001212079 ABIN1112724 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001212079 ABIN2042524 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001212079 ABIN2114268 Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.001212079 ABIN2684580 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001212079 ABIN2684581 Sandwich ELISA Plasma (EDTA), Plasma (citrate), Cell Culture Supernatant, Serum 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.001212079 ABIN1846946 Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001212079 ABIN2116081 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.001212079 ABIN2535385 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see

Top referenced IL-13 ELISA Kit for ELISA

Similar IL-13 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 29 ELISA Kits

General

Antigen Interleukin 13 (IL13) ELISA Kits
Epitope AA 26-131
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(52), (29), (21), (10), (7), (7), (6), (5), (4), (4), (3), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 6 references available
Supplier Log in to see

Product Details IL-13 ELISA Kit

Target details Application Details Handling References for IL-13 Kit (ABIN411312) Images
Purpose For quantitative detection of mouse IL-13 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse IL-13
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- mouse IL-13 antibod - 1
  • Lyophilized recombinant mouse IL-13 standar - 10ng/tubex2
  • Biotinylated anti- mouse IL-13 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: S26-F131

Target details

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Antigen
Alternative Name IL13 (IL13 ELISA Kit Abstract)
Background Interleukin(IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases. Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses. IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy. The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5KDa.
Gene ID 16163
UniProt P20109
Research Area Cancer, Cytokines, Innate Immunity
Pathways JAK-STAT Signaling

Application Details

Product Details IL-13 ELISA Kit Target details Handling References for IL-13 Kit (ABIN411312) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse IL-13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-13 has been precoated onto 96-well plates. Standards(E.coli, S26-F131) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-13 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-13 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 112, Standard deviation: 6.16, CV(%): 5.5
  • Sample 2: n=16, Mean(pg/ml): 355, Standard deviation: 21.3, CV(%): 6
  • Sample 3: n=16, Mean(pg/ml): 628, Standard deviation: 29.52, CV(%): 4.7,
  • Sample 1: n=24, Mean(pg/ml): 149, Standard deviation: 9.24, CV(%): 6.2
  • Sample 2: n=24, Mean(pg/ml): 417, Standard deviation: 29.61, CV(%): 7.1
  • Sample 3: n=24, Mean(pg/ml): 674, Standard deviation: 39.1, CV(%): 5.8
Restrictions For Research Use only

Handling

Product Details IL-13 ELISA Kit Target details Application Details References for IL-13 Kit (ABIN411312) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL-13 Kit (ABIN411312)

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Product cited in:

Li, Li, Zuo, Ke, Yan, Wen, Zhang, Yang: "T-helper type-2 contact hypersensitivity of Balb/c mice aggravated by dibutyl phthalate via long-term dermal exposure." in: PLoS ONE, Vol. 9, Issue 2, pp. e87887, 2014

Background publications

Wang, Shen, Li, Zhang, Luo, Zhong, Jiang, Shen: "Enhanced expression of the decoy receptor IL-13Ralpha2 in macrophages of Schistosoma japonicum-infected mice." in: Chinese medical journal, Vol. 122, Issue 14, pp. 1650-4, 2009

Fichtner-Feigl, Strober, Kawakami, Puri, Kitani: "IL-13 signaling through the IL-13alpha2 receptor is involved in induction of TGF-beta1 production and fibrosis." in: Nature medicine, Vol. 12, Issue 1, pp. 99-106, 2006

Kelly-Welch, Hanson, Boothby, Keegan: "Interleukin-4 and interleukin-13 signaling connections maps." in: Science (New York, N.Y.), Vol. 300, Issue 5625, pp. 1527-8, 2003

Smirnov, Smirnova, Korobko, Frolova: "Tandem arrangement of human genes for interleukin-4 and interleukin-13: resemblance in their organization." in: Gene, Vol. 155, Issue 2, pp. 277-81, 1995

Minty, Chalon, Derocq, Dumont, Guillemot, Kaghad, Labit, Leplatois, Liauzun, Miloux: "Interleukin-13 is a new human lymphokine regulating inflammatory and immune responses." in: Nature, Vol. 362, Issue 6417, pp. 248-50, 1993

Images

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Supplier Images
ELISA image for IL-13 ELISA Kit (Interleukin 13) (ABIN411312) Mouse IL-13 PicoKine ELISA Kit standard curve