anti-Cow (Bovine) IL-4 antibody for Flow Cytometry

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
  • Il-4
Reactivity
Cow (Bovine)
391
208
50
34
24
19
19
14
11
11
9
9
6
6
6
6
5
2
2
1
1
Host
Mouse
263
217
205
7
6
4
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
86
52
32
22
21
12
12
8
8
8
8
8
6
6
6
6
6
4
4
4
4
4
3
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
388
371
254
126
56
46
42
39
39
23
22
21
15
10
9
8
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120253
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN317378 FACS PE Mouse IgG2a Log in to see CC303 2
1 ABIN596088 FACS PE Mouse IgG2a Log in to see
1 ABIN2475007 FACS ELISA Mouse IgG2a Log in to see CC303 4
1 ABIN2475010 FACS FITC Mouse IgG2a Log in to see CC303 4
1 ABIN2475011 FACS PE Mouse IgG2a Log in to see CC303 4
1 ABIN1819708 FACS PE Mouse IgG2a Log in to see CC303
1 ABIN213096 FACS ELISA Biotin Mouse IgG2b Log in to see
1 ABIN1834921 FACS FITC Mouse IgG2a Log in to see CC303
1 ABIN1819707 FACS ELISA Mouse IgG2a Log in to see CC303
1 ABIN2289134 FACS ELISA Biotin Mouse IgG2b Log in to see 10B2403
1 ABIN2289129 FACS ELISA Mouse IgG2a Log in to see 10B2399
1 ABIN2289133 FACS ELISA Mouse IgG2a Log in to see 10B2400
1 ABIN2289131 FACS PE Mouse IgG2a Log in to see 10B2399
1 ABIN639279 FACS ELISA Biotin Mouse IgG2b Log in to see
1 ABIN639278 FACS ELISA Mouse IgG2a Log in to see

Top referenced anti-IL-4 antibody for Flow Cytometry

Similar anti-IL-4 Antibodies

Application / Reactivity Cow (Bovine)
ELISA 11 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 16 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Cow (Bovine)
(391), (208), (50), (34), (24), (19), (19), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (2), (1), (1)
Host Mouse
(263), (217), (205), (7), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(86), (52), (32), (22), (21), (12), (12), (8), (8), (8), (8), (8), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
(388), (371), (254), (126), (56), (46), (42), (39), (39), (23), (22), (21), (15), (10), (9), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN120253) Images
Specificity This antibody is specific for interleukin 4 (IL-4). This reagent may be used as a capture antibody in ELISA assays for bovine IL-4 in combination with SM2242B as a detection reagent, and recombinant bovine IL-4 (SA1007) as a standard.
Purification Affinity chromatography on Protein G
Immunogen Recombinant bovine IL-4. Spleen cells form immunised BALB/c mice were fused with cells of the SP2/O myeloma cell line.
Clone CC313
Isotype IgG1

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN120253) Images back to top
Antigen
Alternative Name Interleukin-4 / IL4 (IL4 Antibody Abstract)
Background IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 280824
NCBI Accession NP_776346
UniProt P30367
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling ProductDetails: References for anti-IL-4 antibody (ABIN120253) Images back to top
Application Notes Flow Cytometry. ELISA: 2 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details ProductDetails: References for anti-IL-4 antibody (ABIN120253) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-IL-4 antibody (ABIN120253)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Abbott, Palmer, Kegerreis, Hetrick, Howard, Hope, Brown: "Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 6702-15, 2005

Hope, Kwong, Thom, Sopp, Mwangi, Brown, Palmer, Wattegedera, Entrican, Howard: "Development of detection methods for ruminant interleukin (IL)-4." in: Journal of immunological methods, Vol. 301, Issue 1-2, pp. 114-23, 2005