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anti-Cow (Bovine) IL-4 antibody for Flow Cytometry

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Cow (Bovine)
407
215
50
24
22
19
14
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Mouse
268
226
219
8
7
2
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
95
53
35
23
23
13
11
8
8
7
7
7
7
6
6
6
6
6
6
4
4
4
4
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
410
374
259
116
58
55
39
37
34
30
24
23
20
19
10
6
6
5
3
3
3
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN120253
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010463598 ABIN317378 FACS PE Mouse IgG2a Log in to see CC303 2
0.0010463598 ABIN596088 FACS PE Mouse IgG2a Log in to see
0.0010463598 ABIN254181 FACS ELISA Biotin Mouse IgG2b Log in to see CC314
0.0010463598 ABIN1819708 FACS PE Mouse IgG2a Log in to see CC303
0.0010463598 ABIN1834921 FACS FITC Mouse IgG2a Log in to see CC303
0.0010463598 ABIN213096 FACS ELISA Biotin Mouse IgG2b Log in to see
0.0010463598 ABIN1819707 FACS ELISA Mouse IgG2a Log in to see CC303
0.0010463598 ABIN217738 FACS ELISA Mouse IgG2a Log in to see
0.0010463598 ABIN2487445 FACS PE Mouse IgG2a Log in to see
0.0010463598 ABIN2487442 FACS ELISA Biotin Mouse IgG2b Log in to see
0.0010463598 ABIN2475010 FACS FITC Mouse IgG2a Log in to see CC303 4
0.0010463598 ABIN2475011 FACS PE Mouse IgG2a Log in to see CC303 4
0.0010463598 ABIN2487443 FACS ELISA Mouse IgG2a Log in to see
0.0010463598 ABIN2475007 FACS ELISA Mouse IgG2a Log in to see CC303 4
0.0010463598 ABIN2289134 FACS ELISA Biotin Mouse IgG2b Log in to see 10B2403
0.0010463598 ABIN2487444 FACS ELISA Mouse IgG2a Log in to see
0.0010463598 ABIN2289131 FACS PE Mouse IgG2a Log in to see 10B2399
0.0010463598 ABIN2289133 FACS ELISA Mouse IgG2a Log in to see 10B2400
0.0010463598 ABIN2289129 FACS ELISA Mouse IgG2a Log in to see 10B2399
0.0010463598 ABIN639279 FACS ELISA Biotin Mouse IgG2b Log in to see

Top referenced anti-IL-4 antibody for Flow Cytometry

Similar anti-IL-4 Antibodies

Application / Reactivity Cow (Bovine)
ELISA 15 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 23 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Cow (Bovine)
(407), (215), (50), (24), (22), (19), (14), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Mouse
(268), (226), (219), (8), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(95), (53), (35), (23), (23), (13), (11), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
(410), (374), (259), (116), (58), (55), (39), (37), (34), (30), (24), (23), (20), (19), (10), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN120253) Images
Specificity This antibody is specific for interleukin 4 (IL-4). This reagent may be used as a capture antibody in ELISA assays for bovine IL-4 in combination with SM2242B as a detection reagent, and recombinant bovine IL-4 (SA1007) as a standard.
Purification Affinity chromatography on Protein G
Immunogen Recombinant bovine IL-4. Spleen cells form immunised BALB/c mice were fused with cells of the SP2/O myeloma cell line.
Clone CC313
Isotype IgG1

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN120253) Images back to top
Antigen
Alternative Name Interleukin-4 / IL4 (IL4 Antibody Abstract)
Background IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 280824
NCBI Accession NP_776346
UniProt P30367
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN120253) Images back to top
Application Notes Flow Cytometry. ELISA: 2 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN120253) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL-4 Antibody (ABIN120253)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Abbott, Palmer, Kegerreis, Hetrick, Howard, Hope, Brown: "Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 6702-15, 2005

Hope, Kwong, Thom, Sopp, Mwangi, Brown, Palmer, Wattegedera, Entrican, Howard: "Development of detection methods for ruminant interleukin (IL)-4." in: Journal of immunological methods, Vol. 301, Issue 1-2, pp. 114-23, 2005