anti-Human IL-4 antibody for Enzyme Immunoassay

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
  • Il-4
Reactivity
Human
390
208
50
34
24
20
19
14
11
11
9
9
6
6
6
6
5
2
2
1
1
Host
Rat
263
218
204
7
6
4
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
86
52
32
22
21
12
12
8
8
8
8
8
6
6
6
6
6
4
4
4
4
4
3
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
388
370
254
126
56
45
42
39
39
23
22
21
15
10
9
8
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN119373
$ 511.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115763 EIA Func IHC (fro) Mouse IgG1 Log in to see 1
1 ABIN112303 EIA Mouse IgG1 Log in to see 31-2
1 ABIN116073 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN116074 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN116075 EIA WB Rabbit Log in to see Polyclonal
1 ABIN116076 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN316608 EIA Biotin Rat IgG1 Log in to see MP4-25D2
1 ABIN5565737 EIA RIA Biotin Rabbit AA 25-153 Log in to see Polyclonal
1 ABIN5565735 EIA RIA Rabbit AA 25-153 Log in to see Polyclonal

Top referenced anti-IL-4 antibody for Enzyme Immunoassay

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Application / Reactivity Human
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Blocking Peptide (BP) 2 Antibodies
Cell Culture (CC) 1 Antibodies
Dot Blot (DB) 3 Antibodies
ELISA 190 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 7 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
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Functional Studies (Func) 11 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 31 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Human
(390), (208), (50), (34), (24), (20), (19), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (2), (1), (1)
Host Rat
(263), (218), (204), (7), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(86), (52), (32), (22), (21), (12), (12), (8), (8), (8), (8), (8), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(388), (370), (254), (126), (56), (45), (42), (39), (39), (23), (22), (21), (15), (10), (9), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN119373) Images
Specificity This antibody is specific for Interleukin - 4 (IL-4). Clone MP4-25D2 is reported to neutralise IL-4 activity. We recommend the use of SM1702A for this purpose.
Purification Affinity chromatography on Protein G
Immunogen Purified CHO cell expressed recombinant human IL-4.
Clone MP4-25D2
Isotype IgG1

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN119373) Images back to top
Antigen
Alternative Name Interleukin-4 / IL4 (IL4 Antibody Abstract)
Background IL-4 is a 20kD cytokine which is expressed by a subpopulation of activated T cells and mast cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 3565
NCBI Accession NP_000580
UniProt P05112
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling ProductDetails: References for anti-IL-4 antibody (ABIN119373) Images back to top
Application Notes Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA. Western Blot: 1/500 - 1/1000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details ProductDetails: References for anti-IL-4 antibody (ABIN119373) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-IL-4 antibody (ABIN119373)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Bie, Yuan, Zhao, Zhang: "Method for estimating the axial intensity derivative in the TIE with higher order intensity derivatives and noise suppression." in: Optics express, Vol. 20, Issue 7, pp. 8186-91, 2012

Patterson, Behbahani, Kabat, Sullivan, OGorman, Landay, Flener, Khan, Yogev, Andersson: "Leukemia inhibitory factor inhibits HIV-1 replication and is upregulated in placentae from nontransmitting women." in: The Journal of clinical investigation, Vol. 107, Issue 3, pp. 287-94, 2001

Krohn, Preis, Prehn: "Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 20, pp. 8186-97, 1998

Kubota, Miller, Imaoka, Kawabata, Fujihashi, McGhee, Kiyono: "Oral immunization with simian immunodeficiency virus p55gag and cholera toxin elicits both mucosal IgA and systemic IgG immune responses in nonhuman primates." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5321-9, 1997

Carvalho, Bacellar, Brownell, Regis, Coffman, Reed: "Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 12, pp. 5949-56, 1994

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN119373) back to top
Supplier Images
Western Blotting (WB) image for anti-IL-4 antibody (Interleukin 4) (ABIN119373) Western blot analysis of human IL-4 recombinant protein probed with RAT ANTI HUMAN IN...