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anti-Human IL-4 antibody for ELISA

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Human
409
218
51
25
22
19
15
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rat
269
229
220
8
7
2
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
95
53
35
23
23
13
11
8
8
7
7
7
7
6
6
6
6
6
6
4
4
4
4
1
1
1
1
1
1
Application
Intracellular Staining (ICS), Neutralization (Neut), ELISA
413
377
262
118
57
57
39
37
34
31
24
22
20
20
10
6
6
5
3
3
3
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN1177267
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012117105 ABIN964769 ELISA WB HRP Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN964778 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN225255 FACS IP IHC ELISA Mouse IgG1 Log in to see 8D4-8
0.0012117105 ABIN1102497 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN964756 IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN255816 FACS IHC (fro) IHC ELISA WB Rat IgG1 Log in to see MP4-25D2
0.0012117105 ABIN217644 FACS IHC (fro) ELISA WB Rat IgG1 Log in to see
0.0012117105 ABIN1102498 IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN218106 ELISA Mouse IgG1 Log in to see 11-60
0.0012117105 ABIN283749 Inhibition ELISA WB Mouse IgG2a Log in to see M6110641
0.0012117105 ABIN465167 ELISA Mouse IgG2a Log in to see
0.0012117105 ABIN1102500 IHC (p) Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN218097 FACS IP ELISA Mouse IgG1, kappa Log in to see 860A 4B3
0.0012117105 ABIN208738 ELISA Biotin Mouse IgG1, kappa Log in to see 860F10H12
0.0012117105 ABIN1905183 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 130-160 Log in to see Polyclonal
0.0012117105 ABIN1905184 FACS IHC ELISA WB APC Rabbit IgG C-Term, AA 130-160 Log in to see Polyclonal
0.0012117105 ABIN1905185 FACS IHC ELISA WB PE Rabbit IgG C-Term, AA 130-160 Log in to see Polyclonal
0.0012117105 ABIN1584604 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0012117105 ABIN2689768 ELISA Biotin Rat IgG1 Log in to see MP4 4
0.0012117105 ABIN283750 ELISA Mouse IgG1 Log in to see M313012

Top referenced anti-IL-4 antibody for ELISA

Similar anti-IL-4 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 7 Antibodies
Dot Blot (DB) 5 Antibodies
ELISA 212 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 7 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 123 Antibodies
Functional Studies (Func) 11 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 28 Antibodies
Immunohistochemistry (IHC) 78 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Human
(409), (218), (51), (25), (22), (19), (15), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Rat
(269), (229), (220), (8), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(95), (53), (35), (23), (23), (13), (11), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS), Neutralization (Neut), ELISA
(413), (377), (262), (118), (57), (57), (39), (37), (34), (31), (24), (22), (20), (20), (10), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN1177267) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-4. Neutralization may be measured with a proliferation assay using 2 ng/mL recombinant human IL-4 (Cat. No. 554605) to stimulate TF-1 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human IL-4 for 60 minutes).
50% Neutralization (ND50) at 4 - 20 ng/mL
>95% Neutralization at 20 - 100 ng/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Purified Recombinant Human IL-4
Clone MP4-25D2
Isotype IgG1

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN1177267) Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Background The MP4-25D2 antibody reacts with human interleukin-4 (IL-4). The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to cross react with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described.
Research Area Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN1177267) Images back to top
Application Notes ELISA: Biotinylated MP4-25D2 antibody (Cat. No. 554483) has been reported to be useful as a detection antibody for sandwich ELISA measuring human IL-4 protein levels. Biotinylated MP4-25D2 antibody can be paired with the purified 8D4-8 antibody (Cat. No. 554515) as the capture antibody, with recombinant human IL-4 (Cat. No. 554605) as the standard. Investigators are encouraged to use the BD OptEIA™ Human IL-4 ELISA (Cat. No. 555194 or 550614) for measuring human IL-4 in serum or plasma samples.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN1177267) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-4 Antibody (ABIN1177267)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Product cited in:

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Ramanathan, Ingram, Sullivan, Greenberg, Reim, Trotta, Le: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." in: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, 1993

Chrétien, Van Kimmenade, Pearce, Banchereau, Abrams: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." in: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, 1989

Kitamura, Tange, Terasawa, Chiba, Kuwaki, Miyagawa, Piao, Miyazono, Urabe, Takaku: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, 1989