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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|Blocking Antibody (Inhibition)||7 Antibodies|
|Dot Blot (DB)||5 Antibodies|
|ELISA (Capture)||3 Antibodies|
|ELISA (Detection)||4 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||2 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||2 Antibodies|
|Enzyme Immunoassay (EIA)||8 Antibodies|
|Flow Cytometry (FACS)||90 Antibodies|
|Functional Studies (Func)||11 Antibodies|
|Immunoassay (IA)||2 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||61 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||10 Antibodies|
|Antigen||Interleukin 4 (IL4) Antibodies|
|Conjugate||This IL-4 antibody is un-conjugated Alternatives|
ELISA (Capture), ELISpot (Capture) (ELISPOT (Capture))
|1 reference available|
|Supplier||Log in to see|
Product Details anti-IL-4 AntibodyTarget Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN281621) Images
|Specificity||The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.|
The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.
Synonyms: Synonyms: Interleukin-4
Target Details IL-4Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN281621) Images back to top
|Alternative Name||IL-4 (IL4 Antibody Abstract)|
|Research Area||Cytokines, Immunology, Virology, Inflammation, Cancer|
Application DetailsProduct Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN281621) Images back to top
Applications reported: The 8D4-8 antibody has been reported for use in capture of human IL-4 by ELISA and in intracellular staining for flow cytometric analysis.
Applications tested: The 8D4-8 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the biotinylated MP4-25D2 (13-7048) antibody for detection and recombinant human IL-4 (14-8049) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate. The fluorochrome-conjugated 8D4-8 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining for flow cytometric analysis. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN281621) Images back to top
|Buffer||aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.|
References for anti-IL-4 Antibody (ABIN281621)Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Bird, Wadhwa, Thorpe: "Development of immunoassays for human interleukin 3 and interleukin 4, some of which discriminate between different recombinant DNA-derived molecules." in: Cytokine, Vol. 3, Issue 6, pp. 562-7, 1992 (PubMed).
ImagesProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN281621) back to top