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anti-Human IL-4 antibody for ELISpot (Detection)

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Human
406
215
50
25
22
19
14
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rat
268
225
220
8
7
2
Clonality
Monoclonal
Conjugate
This IL-4 antibody is conjugated to Biotin
94
53
35
23
23
13
11
8
8
7
7
7
7
6
6
6
6
6
6
4
4
4
4
1
1
1
1
1
1
Application
ELISA (Detection), ELISpot (Detection) (ELISPOT (Detection))
410
374
260
116
58
55
39
37
34
30
24
23
20
20
10
6
6
4
3
3
3
2
2
2
2
1
1
1
1
1
1
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Available images

Catalog No. ABIN279971
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN1721163 ELISA (Detection) ELISPOT (Detection) Biotin Mouse IgG1 Log in to see B-G28

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Application / Reactivity Human
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Blocking Antibody (Inhibition) 7 Antibodies
Dot Blot (DB) 5 Antibodies
ELISA 212 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 4 Antibodies
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ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 123 Antibodies
Functional Studies (Func) 11 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 28 Antibodies
Immunohistochemistry (IHC) 78 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 24 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Human
(406), (215), (50), (25), (22), (19), (14), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Rat
(268), (225), (220), (8), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is conjugated to Biotin
(94), (53), (35), (23), (23), (13), (11), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1)
Application ELISA (Detection), ELISpot (Detection) (ELISPOT (Detection))
(410), (374), (260), (116), (58), (55), (39), (37), (34), (30), (24), (23), (20), (20), (10), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Specificity The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.
Characteristics The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.
Synonyms: Synonyms: Interleukin-4, IL4
Purification Purified
Clone MP4-25D2
Isotype IgG1 kappa

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Applications reported: The MP4-25D2 antibody has been reported for use in detection of human IL-4 by ELISA and for intracellular staining for flow cytometry.

Applications tested: The MP4-25D2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the purified 8D4-8 (14-7049) antibody for capture and recombinant human IL-4 (14-8049) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze.
Storage 4 °C

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
 image for anti-IL-4 antibody (Interleukin 4)  (Biotin) (ABIN279971) Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in th...
 image for anti-IL-4 antibody (Interleukin 4)  (Biotin) (ABIN279971) anti-Interleukin 4 (IL4) antibody (Biotin) (Image 2)