anti-Human IL-4 antibody for Immunohistochemistry

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
  • Il-4
Epitope
AA 122-150
25
22
13
11
10
9
7
6
5
5
4
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
392
207
50
34
24
20
19
14
11
11
9
9
6
6
6
6
5
2
2
1
1
Host
Rabbit
265
218
204
7
6
4
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
85
52
32
22
21
12
12
8
8
8
8
8
6
6
6
6
6
4
4
4
4
4
3
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
386
373
254
125
56
46
43
39
39
23
22
22
15
10
9
8
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN3031478
$ 411.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.894592 ABIN1876850 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2659777 FACS IF IHC PE/Dazzle™ 594 Rat IgG1 kappa Log in to see MP4-25D2 10
1 ABIN1102498 IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN1102497 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN225255 FACS IP IHC ELISA Mouse IgG1 Log in to see 8D4-8
1 ABIN255816 FACS IHC (fro) IHC ELISA WB Rat IgG1 Log in to see MP4-25D2
1 ABIN2665169 IF IHC WB Rat IgG1 kappa Log in to see MP4-25D2 10
1 ABIN964756 IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1584604 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2659778 FACS IF IHC PE/Dazzle™ 594 Rat IgG1 kappa Log in to see MP4-25D2 11
1 ABIN1889568 Neut IHC WB Mouse IgG1 AA 25-153 Log in to see MM0404-4Z33
1 ABIN1958146 IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN1957039 IHC ELISA WB FITC Rabbit IgG Log in to see Polyclonal
1 ABIN1905183 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 130-160 Log in to see Polyclonal
1 ABIN1905184 FACS IHC ELISA WB APC Rabbit IgG AA 130-160 Log in to see Polyclonal
1 ABIN1905185 FACS IHC ELISA WB PE Rabbit IgG AA 130-160 Log in to see Polyclonal
1 ABIN1955805 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2624137 FACS IHC ELISA WB Rabbit IgG AA 130-160 Log in to see Polyclonal
1 ABIN1825562 IP IHC ELISA WB Mouse IgG2a Log in to see NYRhIL4
1 ABIN1960518 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

General

Antigen Interleukin 4 (IL4) Antibodies
Epitope AA 122-150
(25), (22), (13), (11), (10), (9), (7), (6), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(392), (207), (50), (34), (24), (20), (19), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (2), (1), (1)
Host Rabbit
(265), (218), (204), (7), (6), (4)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(85), (52), (32), (22), (21), (12), (12), (8), (8), (8), (8), (8), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(386), (373), (254), (125), (56), (46), (43), (39), (39), (23), (22), (22), (15), (10), (9), (8), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Cross-Reactivity (Details) Expected species reactivity: Primate
Purification Antigen affinity purified
Immunogen A portion of amino acids 122-150 from the human protein was used as the immunogen for this IL-4 antibody.
Isotype Ig Fraction

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Background IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.
UniProt P05112
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Titration of the IL-4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Liquid
Buffer In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot the IL-4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) (Left) Western blot analysis of IL-4 antibody and WiDr lysate; (Right) Western blot a...
Flow Cytometry (FACS) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) IL-4 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a ...
Immunohistochemistry (IHC) image for anti-Interleukin 4 (IL4) (AA 122-150) antibody (ABIN3031478) IL-4 antibody IHC analysis in formalin fixed and paraffin embedded tonsil