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anti-Mouse (Murine) IL-4 antibody for Immunocytochemistry

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Epitope
AA 23-140
23
12
12
10
9
7
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Mouse (Murine)
329
181
50
25
19
15
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rabbit
243
200
152
8
7
2
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
90
47
31
23
18
8
7
6
4
4
4
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
340
303
187
84
56
31
27
26
24
23
20
20
20
17
10
6
6
5
3
3
3
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1862772
$ 356.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010211383 ABIN2689764 ICC Rat IgG1 Log in to see 11B11
0.0010211383 ABIN645727 ICC IP IHC ELISA WB Rat IgG1 Log in to see
0.0010211383 ABIN1853858 ELISpot ICC FACS IF IP IHC ELISA WB FITC Rat IgG1 Log in to see
0.0010211383 ABIN1853859 ELISpot ICC FACS IF IP IHC ELISA WB PE Rat IgG1 Log in to see
0.0010211383 ABIN1853862 ELISpot ICC FACS IF IP IHC ELISA WB Rat IgG1 Log in to see

Similar anti-IL-4 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 96 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 8 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 76 Antibodies
Functional Studies (Func) 10 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunohistochemistry (IHC) 22 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Epitope AA 23-140
(23), (12), (12), (10), (9), (7), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(329), (181), (50), (25), (19), (15), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Rabbit
(243), (200), (152), (8), (7), (2)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(90), (47), (31), (23), (18), (8), (7), (6), (4), (4), (4), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(340), (303), (187), (84), (56), (31), (27), (26), (24), (23), (20), (20), (20), (17), (10), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Sequence MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-HGCDKNHL REIIGILNEV TGEGTPCTEM DVPNVLTATK NTTESELVCR ASKVLRIFYL KHGKTPCLKK NSSVLMELQR LFRAFRCLDS SISCTMNESK STSLKDFLES LKSIMQMDYS
Specificity It has been selected for its ability to recognize IL4 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen UMOD (AA 23-140)
Isotype IgG

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant UMOD (His23~Ser140) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL-4 antibody (Interleukin 4) (AA 23-140) (ABIN1862772) anti-Interleukin 4 (IL4) (AA 23-140) antibody