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anti-Mouse (Murine) IL-4 antibody for Intracellular Staining

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Mouse (Murine)
374
182
50
25
19
15
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rat
247
199
195
8
7
2
Clonality
Monoclonal
Conjugate
This IL-4 antibody is conjugated to PE
93
49
34
23
22
8
8
7
6
4
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
347
344
192
84
56
38
36
31
28
27
24
22
20
20
10
6
6
5
3
3
3
2
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN279098
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008664096 ABIN286622 FACS ICS Alexa Fluor 488 Rat IgG1 kappa Log in to see 11B11
0.0008664096 ABIN282861 FACS ICS APC Rat IgG1 kappa Log in to see 11B11
0.0008664096 ABIN278151 FACS ICS FITC Rat IgG1 kappa Log in to see BVD6-24G2
0.0008664096 ABIN2680450 FACS ICS PE-Cy7 Rat IgG1 kappa Log in to see 11B11
0.0008664096 ABIN283053 FACS ICS PE-Cy7 Rat IgG1 kappa Log in to see BVD6-24G2
0.0008664096 ABIN2680454 FACS ICS PerCP-eFluor® 710 Rat IgG1 kappa Log in to see 11B11
0.0008664096 ABIN1002647 ICS IP IHC ELISA WB Rat IgG1 Log in to see NYRmIL-4
0.0008664096 ABIN2689761 ICS ELISA WB Rat IgG1 Log in to see 11B11
0.0008664096 ABIN2748988 ELISpot ICS Neut ELISA WB Rat IgG1 Log in to see 11B11
0.0008664096 ABIN808092 ICS IP IHC TCC ELISA Rat Log in to see NYRmIL-4

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Application / Reactivity Mouse (Murine)
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General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Mouse (Murine)
(374), (182), (50), (25), (19), (15), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Rat
(247), (199), (195), (8), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is conjugated to PE
(93), (49), (34), (23), (22), (8), (8), (7), (6), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(347), (344), (192), (84), (56), (38), (36), (31), (28), (27), (24), (22), (20), (20), (10), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Specificity The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.
Characteristics The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.
Synonyms: Synonyms: Interleukin-4
Clone 11B11
Isotype IgG1 kappa

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Applications reported: This 11B11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This 11B11 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining of Th2 polarized and stimulated mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining of Th2 polarized and stimulated mouse splenocytes. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test.
Comment

Excitation/Emission wavelength: 496 nm/576 nm

Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light sensitive material.
Storage 4 °C

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL-4 antibody (Interleukin 4)  (PE) (ABIN279098) anti-Interleukin 4 (IL4) antibody (PE)