anti-Mouse (Murine) IL-4 antibody for Blocking Antibody

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
  • Il-4
Reactivity
Mouse (Murine)
393
207
50
34
24
20
19
14
11
11
9
9
6
6
6
6
5
2
2
1
1
Host
Rabbit
265
218
204
7
6
4
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
85
52
32
22
21
12
12
8
8
8
8
8
6
6
6
6
6
4
4
4
4
4
3
2
2
2
2
1
1
Application
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
386
373
255
126
56
46
43
39
39
23
22
22
15
10
8
8
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1002644
$ 374.22
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN289356 Inhibition ELISA WB Rabbit Log in to see Polyclonal

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Mouse (Murine)
(393), (207), (50), (34), (24), (20), (19), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (2), (1), (1)
Host Rabbit
(265), (218), (204), (7), (6), (4)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(85), (52), (32), (22), (21), (12), (12), (8), (8), (8), (8), (8), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
(386), (373), (255), (126), (56), (46), (43), (39), (39), (23), (22), (22), (15), (10), (8), (8), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Purification Antigen affinity purified polyclol antibody
Sterility Sterile filtered
Immunogen recombint mIL4 (murine Interleukin4)
Isotype IgG

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin 4 (IL4 Antibody Abstract)
Background Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL4 (murine Interleukin4). Anti-Murine IL-4 specific antibody was purified by affinity chromatography employing immobilized mIL-4 matrix.
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes To yield one-half maximal inhibition [ND₅₀] of the biological activity of mIL-4 (5 ng/µl), a concentration of 0.78 - 1.16 µg/µl of this antibody is required. To detect mIL-4 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-4. To detect mIL-4 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Murine IL-4 (PK-AB815-61410MB) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-4. To detect mIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunihostochemistry: NA. To detect mIL-4 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Murine IL-4 (PK-AB815-61410MB) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-4. To detect mIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Lyophilized
Concentration To yield one-half maximal inhibition [ND₅₀] of the biological activity of mIL-4 (5 ng/µl), a concentration of 0.78 - 1.16 µg/µl of this antibody is required.
Buffer A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
Handling Advice The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
Storage -20 °C
Expiry Date 24 months