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anti-Mouse (Murine) IL-4 antibody for Neutralization

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
  • Il-4
Reactivity
Mouse (Murine)
402
213
52
27
25
23
19
14
12
11
9
7
6
6
6
6
6
2
2
1
Host
Rat
269
215
214
8
7
2
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
92
53
33
22
21
11
11
8
8
7
7
7
6
6
6
6
6
4
4
4
4
4
3
2
1
1
1
1
Application
Neutralization (Neut), ELISA
403
389
248
127
57
46
46
36
36
23
22
20
15
10
10
8
6
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN1177294
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177017 FACS Neut ELISA WB Rat IgG1 Log in to see 11B11 5
1 ABIN465884 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal
1 ABIN465839 Neut ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2689762 Neut ELISA WB Rat IgG2b Log in to see BVD4 4
1 ABIN2689763 Neut ELISA WB Rat IgG2b Log in to see BVD4 1
1 ABIN218110 Neut ELISA Rat IgG2b Log in to see
1 ABIN218111 Neut ELISA Rat IgG2b Log in to see
1 ABIN208728 Func Neut ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN218113 FACS IF Neut ELISA WB Rat IgG2b, kappa Log in to see
1 ABIN1983778 Neut WB Rat IgG1 AA 23-140 Log in to see RM0274-2L55
1 ABIN346573 Neut ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2289250 Neut ELISA Rat IgG2b Log in to see 5J334
1 ABIN2289225 Neut ELISA Rat IgG2b Log in to see 5J334
1 ABIN2289229 FACS IF Neut ELISA WB Rat IgG2b kappa Log in to see 5K310
1 ABIN2748988 ELISpot ICS Neut ELISA WB Rat IgG1 Log in to see 11B11
1 ABIN217234 Neut ELISA Rat IgG2b Log in to see
1 ABIN2289208 Neut ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2289149 Neut ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2289098 Neut ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
1 ABIN2289100 Neut ELISA WB APC Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL-4 antibody for Neutralization

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Application / Reactivity Mouse (Murine)
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Blocking Antibody (Inhibition) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Dot Blot (DB) 1 Antibodies
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ELISpot 3 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 104 Antibodies
Functional Studies (Func) 9 Antibodies
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Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies
Immunohistochemistry (IHC) 42 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Mouse (Murine)
(402), (213), (52), (27), (25), (23), (19), (14), (12), (11), (9), (7), (6), (6), (6), (6), (6), (2), (2), (1)
Host Rat
(269), (215), (214), (8), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(92), (53), (33), (22), (21), (11), (11), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Application Neutralization (Neut), ELISA
(403), (389), (248), (127), (57), (46), (46), (36), (36), (23), (22), (20), (15), (10), (10), (8), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN1177294) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-4. Neutralization activity may be measured with a proliferation assay using 1 ng/mL recombinant mouse IL-4 (Cat. No. 550067) to stimulate D36 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-4 for 60 minutes).
50% Neutralization (ND50) at 1.5 - 75 ng/mL
>95% Neutralization at 0.2 - 1.1 µg/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Recombinant Mouse IL-4
Clone BVD4-1D11
Isotype IgG2b

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN1177294) Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Background The BVD4-1D11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD4-1D11 hybridoma was recombinant mouse IL-4. This is a neutralizing antibody.
Research Area Cancer
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN1177294) Images back to top
Application Notes ELISA: The purified format of BVD4-1D11 antibody (Cat. No. 554387) can be useful as a capture antibody in a sandwich ELISA for measuring mouse IL-4. Purified BVD4-1D11 antibody can be paired with the biotinylated BVD6-24G2 antibody (Cat. No. 554390) as the detecting antibody, with recombinant mouse IL-4 (Cat. No. 550067) as the standard. For testing mouse IL-4 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Mouse IL-4 Set (Cat. No. 555232).
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN1177294) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-4 Antibody (ABIN1177294)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Product cited in:

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Litton, Sander, Murphy, OGarra, Abrams: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, 1994

Schlaak, Schmitt, Hüls, Meyer zum Büschenfelde, Fleischer: "A sensitive and specific bioassay for the detection of human interleukin-10." in: Journal of immunological methods, Vol. 168, Issue 1, pp. 49-54, 1994

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992