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anti-Pig (Porcine) IL-4 antibody for Western Blotting

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Epitope
AA 25-133
23
12
12
10
9
7
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Wild boar (Sus scrofa), Pig (Porcine)
329
182
50
25
19
14
7
7
7
6
6
5
4
3
3
3
2
2
Host
Rabbit
243
200
152
8
7
2
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
90
47
31
23
18
8
7
6
4
4
4
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
340
303
187
84
56
31
27
26
24
23
20
20
20
17
10
6
6
5
3
3
3
2
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN1859429
$ 415.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011806369 ABIN596086 WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN2624122 ELISA WB Biotin Rabbit IgG AA 25-133 Log in to see Polyclonal
0.0011806369 ABIN2624125 WB FITC Rabbit IgG AA 25-133 Log in to see Polyclonal
0.0011806369 ABIN2289125 WB Rabbit IgG Log in to see Polyclonal

Similar anti-IL-4 Antibodies

Application / Reactivity Wild boar (Sus scrofa) Pig (Porcine)
ELISA 1 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 1 Antibodies
Western Blotting (WB) 1 Antibodies 5 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 4 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Epitope AA 25-133
(23), (12), (12), (10), (9), (7), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Wild boar (Sus scrofa), Pig (Porcine)
(329), (182), (50), (25), (19), (14), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2)
Host Rabbit
(243), (200), (152), (8), (7), (2)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(90), (47), (31), (23), (18), (8), (7), (6), (4), (4), (4), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(340), (303), (187), (84), (56), (31), (27), (26), (24), (23), (20), (20), (20), (17), (10), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- HKCDIT LQEIIKTLNI LTARKNSCME LPVTDVFAAP ENTTEKETFC RASTVLRHIY RHHTCMKSLL SGLDRNLSSM ANMTCSVHEA KKSTLKDFLE RLKTIMKEKY SKC
Specificity It has been selected for its ability to recognize IL4 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen IL4 (AA 25-133)
Isotype IgG

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant IL4 (His25~Cys133) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL-4 antibody (Interleukin 4) (AA 25-133) (ABIN1859429) anti-Interleukin 4 (IL4) (AA 25-133) antibody