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anti-Rabbit IL-4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Epitope
AA 26-147
23
12
12
12
10
9
7
5
5
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Rabbit
407
215
50
25
22
19
14
7
7
6
6
6
5
4
3
3
3
2
2
1
Host
Guinea Pig
268
226
220
8
6
2
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
95
53
35
23
23
13
11
8
8
7
7
7
7
6
6
6
6
6
6
4
4
4
4
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
409
373
260
116
58
54
39
36
34
29
24
23
20
20
10
6
6
5
3
3
3
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN1859427
$ 376.20
Plus shipping costs $45.00

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Application / Reactivity Rabbit
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Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Western Blotting (WB) 4 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Epitope AA 26-147
(23), (12), (12), (12), (10), (9), (7), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Rabbit
(407), (215), (50), (25), (22), (19), (14), (7), (7), (6), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Guinea Pig
(268), (226), (220), (8), (6), (2)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(95), (53), (35), (23), (23), (13), (11), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(409), (373), (260), (116), (58), (54), (39), (36), (34), (29), (24), (23), (20), (20), (10), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Sequence MGHHHHHHSG SEF-RGDII LPEVIKTLNI LTERKTPCTK LMIADALAVP KNTTEREAVC RAATALRQFY LHHKVSWCFK EHGELGDLRL LRGLDRNLCS MAKLSNCPGK EARQTTLEDF LDRLKTAMQE KYSKRQS
Specificity It has been selected for its ability to recognize IL4 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen IL4 (AA 26-147)
Isotype IgG

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant IL4 (Arg26~Ser147) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-IL-4 antibody (Interleukin 4) (AA 26-147) (ABIN1859427) anti-Interleukin 4 (IL4) (AA 26-147) antibody