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|Antigen||Interleukin 4 (IL4) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This IL-4 antibody is conjugated to Biotin Alternatives|
ELISA, Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-IL-4 AntibodyTarget Details IL-4 Application Details Handling Images
E.coli derived recombinant Rat IL-4.
Type of Immunogen: Cell extract
Target Details IL-4Product Details anti-IL-4 Antibody Application Details Handling Images back to top
|Alternative Name||IL4 (IL4 Antibody Abstract)|
Name/Gene ID: IL4
Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4
|Research Area||Immunology, Adaptive Immunity, Cytokines, Innate Immunity|
|Pathways||JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation|
Application DetailsProduct Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Rat IL-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-4. To detect Rat IL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat IL-4 (LS-C104621) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-4. Western Blot: To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
|Reconstitution||Sterile PBS containing 0.1 % BSA|
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top