anti-Rat (Rattus) IL-4 antibody for ELISA

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
  • Il-4
Reactivity
Rat (Rattus)
383
204
49
34
24
20
19
14
11
11
9
9
6
6
6
6
5
2
2
1
Host
Rabbit
254
217
201
7
6
4
Clonality
Polyclonal
Conjugate
This IL-4 antibody is conjugated to Biotin
82
51
31
21
21
12
12
8
8
8
8
7
6
6
6
6
6
4
4
4
4
4
3
2
2
2
2
1
Application
ELISA, Western Blotting (WB)
381
368
249
121
56
46
42
36
36
22
22
20
15
10
10
8
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN465481
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2689770 ELISA Biotin Mouse IgG1, kappa Log in to see B11 3
1 ABIN1584604 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN465482 ELISA WB Rabbit Log in to see Polyclonal
1 ABIN3201266 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 61-111 Log in to see
1 ABIN2689765 BR Neut ELISA Mouse IgG1, kappa Log in to see OX 4
1 ABIN2624123 ELISA WB Biotin Rabbit IgG AA 61-111 Log in to see Polyclonal
1 ABIN2624126 ELISA WB FITC Rabbit IgG AA 61-111 Log in to see Polyclonal
1 ABIN2624124 ELISA WB Biotin Mouse IgG AA 61-111 Log in to see
1 ABIN2624127 ELISA WB FITC Mouse IgG AA 61-111 Log in to see
1 ABIN208722 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2624117 ELISA WB Rabbit IgG AA 61-111 Log in to see Polyclonal
1 ABIN2624115 ELISA WB Mouse IgG AA 61-111 Log in to see
1 ABIN1172196 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2748985 ELISpot ELISA Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2475017 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2475018 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN1002645 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2289240 ELISA WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN2289238 ELISA WB FITC Rabbit IgG Log in to see Polyclonal
1 ABIN2289261 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL-4 antibody for ELISA

Similar anti-IL-4 Antibodies

Application / Reactivity Rat (Rattus)
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Blocking Reagent (BR) 1 Antibodies
ELISA 36 Antibodies
ELISpot 4 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Functional Studies (Func) 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 40 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Rat (Rattus)
(383), (204), (49), (34), (24), (20), (19), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (2), (1)
Host Rabbit
(254), (217), (201), (7), (6), (4)
Clonality
Polyclonal
Conjugate This IL-4 antibody is conjugated to Biotin
(82), (51), (31), (21), (21), (12), (12), (8), (8), (8), (8), (7), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1)
Application ELISA, Western Blotting (WB)
(381), (368), (249), (121), (56), (46), (42), (36), (36), (22), (22), (20), (15), (10), (10), (8), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Specificity Rat IL-4
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Rat IL-4.

Type of Immunogen: Cell extract

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL4 (IL4 Antibody Abstract)
Background Name/Gene ID: IL4
Family: Hormone

Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4
Gene ID 3565
UniProt P05112
Research Area Immunology, Adaptive Immunity, Cytokines, Innate Immunity
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Rat IL-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-4. To detect Rat IL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat IL-4 (LS-C104621) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-4. Western Blot: To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Format Lyophilized
Reconstitution Sterile PBS containing 0.1 % BSA
Concentration Lot specific
Buffer Lyophilized from PBS, pH 7.2
Handling Advice Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling back to top
Supplier Images
 image for anti-IL-4 antibody (Interleukin 4)  (Biotin) (ABIN465481) anti-Interleukin 4 (IL4) antibody (Biotin)
 image for anti-IL-4 antibody (Interleukin 4)  (Biotin) (ABIN465481) anti-Interleukin 4 (IL4) antibody (Biotin) (Image 2)