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anti-Rat (Rattus) IL-4 antibody for Flow Cytometry

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Rat (Rattus)
410
218
50
25
22
19
15
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Mouse
269
230
219
8
7
2
Clonality
Monoclonal
Conjugate
This IL-4 antibody is conjugated to EFluor 660
95
53
35
23
23
13
11
8
8
7
7
7
7
6
6
6
6
6
6
4
4
4
4
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
414
377
261
118
58
57
39
37
34
31
24
22
20
20
10
6
6
5
3
3
3
2
2
2
2
2
1
1
1
1
1
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Available images

Catalog No. ABIN2680458
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010482287 ABIN2663792 FACS PE Mouse IgG1 kappa Log in to see OX-81 3
0.0010482287 ABIN2663793 FACS PE Mouse IgG1 kappa Log in to see OX-81 3
0.0010482287 ABIN2680455 Func FACS ICS WB Mouse IgG1 kappa Log in to see OX81

Top referenced anti-IL-4 antibody for Flow Cytometry

Similar anti-IL-4 Antibodies

Application / Reactivity Rat (Rattus)
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ELISA 36 Antibodies
ELISpot 4 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Functional Studies (Func) 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Intracellular Staining (ICS) 2 Antibodies
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General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Rat (Rattus)
(410), (218), (50), (25), (22), (19), (15), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Mouse
(269), (230), (219), (8), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is conjugated to EFluor 660
(95), (53), (35), (23), (23), (13), (11), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(414), (377), (261), (118), (58), (57), (39), (37), (34), (31), (24), (22), (20), (20), (10), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN2680458) Images
Specificity This OX81 monoclonal antibody reacts with rat interleukin (IL)-4. Also known as BCDF, BCGF-1 and BSF-1, IL-4 is secreted by activated Th2 and natural killer T cells. This cytokine is also produced by Th1 and mast cells albeit to a lesser extent. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of MHC class II and CD23.

The OX81 antibody has been reported to exhibit neutralizing activity.
Characteristics This OX81 monoclonal antibody reacts with rat interleukin (IL)-4. Also known as BCDF, BCGF-1 and BSF-1, IL-4 is secreted by activated Th2 and natural killer T cells. This cytokine is also produced by Th1 and mast cells albeit to a lesser extent. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of MHC class II and CD23. The OX81 antibody has been reported to exhibit neutralizing activity.
Synonyms: Synonyms: Interleukin 4
Clone OX81
Isotype IgG1 kappa

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN2680458) Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN2680458) Images back to top
Application Notes Applications reported: This OX81 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This OX81 antibody has been tested by intracellular flow cytometric analysis of activated rat splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN2680458) Images back to top
Concentration 0.2 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

References for anti-IL-4 Antibody (ABIN2680458)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Chakir, Wang, Lefebvre, Webb, Scott: "T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3." in: Journal of immunological methods, Vol. 278, Issue 1-2, pp. 157-69, 2003

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN2680458) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL-4 antibody (Interleukin 4)  (EFluor 660) (ABIN2680458) anti-Interleukin 4 (IL4) antibody (EFluor 660)