Sigmodon hispidus (Hispid cotton rat) IL-4 ELISA Kit

Recommended IL-4 Product (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) ELISA Kits
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
  • Il-4
Reactivity
Sigmodon hispidus (Hispid cotton rat)
Alternatives
Kits with alternative reactivity to:
78
46
45
22
15
13
13
11
11
11
9
7
5
4
4
3
2
1
Method Type
Sandwich ELISA
Application
ELISA
Options
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Catalog No. ABIN4885948
$ 728.20
Plus shipping costs $45.00

General

Antigen Interleukin 4 (IL4) ELISA Kits
Reactivity Sigmodon hispidus (Hispid cotton rat)
Kits with alternative reactivity to:
(78), (46), (45), (22), (15), (13), (13), (11), (11), (11), (9), (7), (5), (4), (4), (3), (2), (1)
Method Type Sandwich ELISA
Application ELISA
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Product Details IL-4 ELISA Kit

Target details Application Details Handling Images
Purpose Custom Cotton Rat (sigmodon) IL-4 ELISA Kit.
Sample Type Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell and Tissue Lysate
Detection Method Colorimetric
Analytical Method Quantitative
Specificity The antibody pair provided in this kit recognizes Cotton rat (Sigmodon) IL-4.
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IL-4 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name IL-4 (IL4 ELISA Kit Abstract)
UniProt Q91ZL2
Research Area Virology, Immunology, Cytokines, Cancer, Inflammation
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details IL-4 ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details IL-4 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months