anti-Human IL-6 Receptor antibody for Immunofluorescence (fixed cells)

Recommended IL-6 Receptor Antibody (supplied by: Log in to see )

Antigen
Interleukin-6 Receptor (IL6R) Antibodies
  • IL6R
  • il-6ra
  • CD126
  • IL-6R-1
  • IL-6RA
  • IL6Q
  • IL6RA
  • IL6RQ
  • gp80
  • IL6R1
  • Il6ra
  • IL-6Ralpha
  • IL-6R
  • Il6r
  • interleukin 6 receptor
  • interleukin 6 receptor-like
  • interleukin 6 receptor, alpha
  • IL6R
  • il6r
  • Il6r
  • LOC100347752
  • Il6ra
Epitope
AA 1-365
24
19
17
15
12
7
6
6
6
6
5
5
5
5
5
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
182
97
46
1
Host
Mouse
130
93
52
23
2
Clonality
Monoclonal
Conjugate
This IL-6 Receptor antibody is un-conjugated
26
15
15
10
10
9
7
7
5
5
5
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Cell Culture (CC), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
132
128
103
71
40
24
23
22
17
13
10
9
9
8
8
6
4
2
2
2
2
1
1
Supplier
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Catalog No. ABIN1996687
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2935538 ELISA IF/ICC IHC WB Rabbit AA 216-356 Log in to see Polyclonal

General

Antigen Interleukin-6 Receptor (IL6R) Antibodies
Epitope AA 1-365
(24), (19), (17), (15), (12), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(182), (97), (46), (1)
Host Mouse
(130), (93), (52), (23), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-6 Receptor antibody is un-conjugated
(26), (15), (15), (10), (10), (9), (7), (7), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cell Culture (CC), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
(132), (128), (103), (71), (40), (24), (23), (22), (17), (13), (10), (9), (9), (8), (8), (6), (4), (2), (2), (2), (2), (1), (1)
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Product Details anti-IL-6 Receptor Antibody

Target Details IL-6 Receptor Application Details Handling Images
Specificity Human IL-6R / CD126
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-6R / CD126 (rh IL-6R / CD126, ABIN2002778 NP_000556.1, Met1-Pro365,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human IL-6R / CD126 protein (ABIN2002778)
Clone 18
Isotype IgG1

Target Details IL-6 Receptor

Product Details anti-IL-6 Receptor Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL6R (IL6R Antibody Abstract)
Background Interleukin 6 receptor subunit alpha, also known as IL6R1, CD126, and IL6Ra, is a type I transmembrane cytokine receptor and lacks a tyrosine/kinase domain within the cytoplasmic region, unlike other growth factor receptors. The IL6 receptor is composed of two different subunits, IL6R that binds IL6 with low affinity, and ubiquitously expressed Glycoprotein 130 (gp130) which acts as a common signal-transducing receptor and binds the IL-6/IL-6R complex with high affinity. Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. The soluble form of IL6R arises from proteolytic cleavage of membrane-bound IL6Rα, and acts agonistically by making the IL6 ligand accessible to the signal transducer gp130. Dysregulated production of IL6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies such as multiple myeloma, rheumatoid arthritis, or osteoporosis. It has been reported that a humanized anti-IL6R monoclonal antibody is a promising agent applicable to the therapeutic approach for IL6 driven diseases.
NCBI Accession NP_000556.1
Research Area Stem Cells, Hematopoietic Progenitors, Innate Immunity, Cytokines, Immunology
Pathways JAK-STAT Signaling, Autophagy, Growth Factor Binding

Application Details

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Handling Images back to top
Application Notes ELISA(Cap): 0.5-4 μg/mL In a sandwich ELISA, it can be used as capture antibody.
ICC/IF: 10-25 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Interleukin-6 Receptor (IL6R) (AA 1-365) antibody (ABIN1996687) Immunofluorescence staining of Human IL6R in Hela cells. Cells were fixed with 4% PFA...