anti-Human IL-6 Receptor antibody for Western Blotting

Recommended IL-6 Receptor Antibody (supplied by: Log in to see )

Antigen
Interleukin-6 Receptor (IL6R) Antibodies
  • IL6R
  • il-6ra
  • CD126
  • IL-6R-1
  • IL-6RA
  • IL6Q
  • IL6RA
  • IL6RQ
  • gp80
  • IL6R1
  • Il6ra
  • IL-6Ralpha
  • IL-6R
  • Il6r
  • interleukin 6 receptor
  • interleukin 6 receptor, alpha
  • IL6R
  • il6r
  • Il6r
  • Il6ra
Epitope
AA 20-339
24
19
16
15
10
7
7
6
6
6
5
5
5
5
5
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
183
88
45
1
Host
Mouse
130
95
45
21
1
Clonality
Monoclonal
Conjugate
This IL-6 Receptor antibody is un-conjugated
26
15
15
10
9
8
6
6
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
132
120
102
71
40
24
24
22
16
13
10
10
8
8
8
5
4
2
2
2
2
2
1
Supplier
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Catalog No. ABIN4899502
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2432864 ABIN4899503 CyTOF ELISA (Capture) FACS Neut WB Mouse IgG1 AA 20-339 Log in to see 17506 15
3.2432864 ABIN951184 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
3.2432864 ABIN1873216 IHC WB Rabbit IgG Log in to see Polyclonal
3.2432864 ABIN4325584 IF IHC IHC (p) WB Mouse IgG1 Log in to see 2F4
3.2432864 ABIN3222753 WB Rabbit Log in to see Polyclonal
3.2432864 ABIN517051 FACS WB Mouse AA 1-468, full length Log in to see Polyclonal
3.2432864 ABIN1175051 ICC IHC IP WB Rabbit IgG AA 216-356 Log in to see Polyclonal
3.2432864 ABIN2404334 IHC WB Rabbit IgG Log in to see Polyclonal
3.2432864 ABIN517052 FACS WB Mouse AA 1-468, full length Log in to see Polyclonal
3.2432864 ABIN517053 WB Rabbit AA 1-468, full length Log in to see Polyclonal
3.2432864 ABIN3015522 IHC WB Rabbit Log in to see Polyclonal
1 ABIN395681 ELISA WB Mouse IgG2b kappa AA 21-121 Log in to see 2G6 5
1 ABIN5518926 WB Rabbit IgG AA 379-419, C-Term Log in to see Polyclonal
1 ABIN185570 ELISA WB Goat Internal Region Log in to see Polyclonal 1
1 ABIN959335 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2656901 ELISA WB APC Mouse IgG1 kappa Log in to see UV4 4
1 ABIN1583038 IHC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1828212 ELISA WB Mouse IgG2b, kappa AA 21-120 Log in to see 2G6
1 ABIN2609966 IF IHC (p) WB Mouse IgG1 Log in to see 2F4
1 ABIN2609965 WB Mouse IgG2a Log in to see 1B5

General

Antigen Interleukin-6 Receptor (IL6R) Antibodies
Epitope AA 20-339
(24), (19), (16), (15), (10), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(183), (88), (45), (1)
Host Mouse
(130), (95), (45), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-6 Receptor antibody is un-conjugated
(26), (15), (15), (10), (9), (8), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
(132), (120), (102), (71), (40), (24), (24), (22), (16), (13), (10), (10), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1)
Pubmed 15 references available
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Product Details anti-IL-6 Receptor Antibody

Target Details IL-6 Receptor Application Details Handling References for anti-IL-6 Receptor antibody (ABIN4899502) Images
Specificity Detects human IL-6 R in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha , recombinant mouse (rm) IL-1 alpha , rhIL-1 beta , rmIL-1 beta , rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6 R, Leu20-Asp339, Accession # P08887
Clone 17506
Isotype IgG1

Target Details IL-6 Receptor

Product Details anti-IL-6 Receptor Antibody Application Details Handling References for anti-IL-6 Receptor antibody (ABIN4899502) Images back to top
Antigen
Alternative Name IL-6 R alpha (IL6R Antibody Abstract)
Gene ID 3570
UniProt P08887
Research Area Stem Cells, Hematopoietic Progenitors, Immunology, Cytokines, Innate Immunity
Pathways JAK-STAT Signaling, Autophagy, Growth Factor Binding

Application Details

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Handling References for anti-IL-6 Receptor antibody (ABIN4899502) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Neutralization 0.04-0.2 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 2-8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details References for anti-IL-6 Receptor antibody (ABIN4899502) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-IL-6 Receptor antibody (ABIN4899502)

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details Handling Images back to top
Product cited in:

Uchibori, Matsuda, Shimodaira, Sugano, Uehara, Honda: "IL-6 trans-signaling is another pathway to upregulate Osteopontin." in: Cytokine, Vol. 90, pp. 88-95, 2016 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Munir, Luu, Clarke, Nash, McGettrick: "Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium." in: PLoS ONE, Vol. 11, Issue 5, pp. e0155161, 2016 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Sommer, Fabri: "Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130395, 2015 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Zielinski, Mele, Aschenbrenner, Jarrossay, Ronchi, Gattorno, Monticelli, Lanzavecchia, Sallusto: "Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β." in: Nature, Vol. 484, Issue 7395, pp. 514-8, 2012 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Nieman, Kenny, Penicka, Ladanyi, Buell-Gutbrod, Zillhardt, Romero, Carey, Mills, Hotamisligil, Yamada, Peter, Gwin, Lengyel: "Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth." in: Nature medicine, Vol. 17, Issue 11, pp. 1498-503, 2011 Method employed by authors: In vivo Studies (in vivo) (Sample species: Human).

Ara, Song, Shimada, Keshelava, Russell, Metelitsa, Groshen, Seeger, DeClerck: "Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells." in: Cancer research, Vol. 69, Issue 1, pp. 329-37, 2009 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Duhen, Geiger, Jarrossay, Lanzavecchia, Sallusto: "Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells." in: Nature immunology, Vol. 10, Issue 8, pp. 857-63, 2009 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Kolliputi, Waxman: "IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation." in: American journal of respiratory cell and molecular biology, Vol. 40, Issue 3, pp. 314-24, 2009 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Acosta-Rodriguez, Napolitani, Lanzavecchia, Sallusto: "Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells." in: Nature immunology, Vol. 8, Issue 9, pp. 942-9, 2007 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Ammit, Moir, Oliver, Hughes, Alkhouri, Ge, Burgess, Black, Roth: "Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 292, Issue 1, pp. L199-206, 2007 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Mozaffarian, Rimm, King, Lawler, McDonald, Levy: "trans fatty acids and systemic inflammation in heart failure." in: The American journal of clinical nutrition, Vol. 80, Issue 6, pp. 1521-5, 2004 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Hurst, McLoughlin, Monslow, Owens, Morgan, Fuller, Topley, Jones: "Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 9, pp. 5244-51, 2002 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Erices, Conget, Rojas, Minguell: "Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells." in: Experimental cell research, Vol. 280, Issue 1, pp. 24-32, 2002 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Hurst, Wilkinson, McLoughlin, Jones, Horiuchi, Yamamoto, Rose-John, Fuller, Topley, Jones: "Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation." in: Immunity, Vol. 14, Issue 6, pp. 705-14, 2001 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Asou, Said, Yang, Munker, Park, Kamada, Koeffler: "Mechanisms of growth control of Kaposi's sarcoma-associated herpes virus-associated primary effusion lymphoma cells." in: Blood, Vol. 91, Issue 7, pp. 2475-81, 1998 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details Handling References for anti-IL-6 Receptor antibody (ABIN4899502) back to top
Supplier Images
Neutralization (Neut) image for anti-Interleukin-6 Receptor (IL6R) (AA 20-339) antibody (ABIN4899502) IL-6 R alpha Enhancement of IL-6-dependent Inhibition of Cell Proliferation and Neutr...
Flow Cytometry (FACS) image for anti-Interleukin-6 Receptor (IL6R) (AA 20-339) antibody (ABIN4899502) Detection of IL-6 R alpha in U937 Human Cell Line by Flow Cytometry. U937 human histi...
Western Blotting (WB) image for anti-Interleukin-6 Receptor (IL6R) (AA 20-339) antibody (ABIN4899502) Detection of Recombinant Human IL-6 R alpha by Western Blot. Western blot shows 25 ng...