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anti-Mouse (Murine) IL-6 Receptor antibody for Flow Cytometry

Recommended IL-6 Receptor Antibody (supplied by: Log in to see )

Antigen
Interleukin-6 Receptor (IL6R) Antibodies
  • CD126
  • gp80
  • IL-6R
  • IL-6R-1
  • IL-6RA
  • il-6ra
  • IL-6Ralpha
  • IL6Q
  • Il6r
  • IL6R
  • IL6R1
  • IL6RA
  • Il6ra
  • IL6RQ
Reactivity
Mouse (Murine)
130
57
43
Host
Rat
86
83
22
15
1
Clonality
Monoclonal
Conjugate
This IL-6 Receptor antibody is conjugated to PE-Cy7
22
14
11
10
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
86
76
72
27
22
15
12
11
11
11
10
9
8
6
4
2
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2677439
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30190992 ABIN2214284 FACS PE Rabbit Log in to see
0.30190992 ABIN2214282 FACS APC Rabbit Log in to see
0.0010888148 ABIN282322 Func FACS Rat IgG2b Log in to see D7715A7
0.0010888148 ABIN595450 FACS APC Rat IgG2a Extracellular Domain Log in to see
0.0010888148 ABIN2665904 FACS IHC Rat IgG2b kappa Log in to see D7715A7 11
0.0010888148 ABIN2665905 FACS IHC Rat IgG2b kappa Log in to see D7715A7 11
0.0010888148 ABIN339558 FACS PE Rat IgG2a Extracellular Domain Log in to see
0.0010888148 ABIN327366 FACS IHC (fro) ELISA WB Rat IgG2a Extracellular Domain Log in to see
0.0010888148 ABIN2289582 IC FACS Rat IgG2a AA 20-357 Log in to see 8H130
0.0010888148 ABIN438583 BR FACS IP Neut Rat IgG2 Log in to see AP-MAB0848
0.0010888148 ABIN278701 FACS PE Rat IgG2b Log in to see D7715A7
0.0010888148 ABIN1853997 FACS PE Rat IgG2b Log in to see
0.0010888148 ABIN1853996 FACS Biotin Rat IgG2b Log in to see
0.0010888148 ABIN1854000 FACS Rat IgG2b Log in to see

Top referenced anti-IL-6 Receptor antibody for Flow Cytometry

Similar anti-IL-6 Receptor Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies
ELISA 16 Antibodies
ELISA (Capture) 4 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Functional Studies (Func) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 19 Antibodies

General

Antigen Interleukin-6 Receptor (IL6R) Antibodies
Reactivity Mouse (Murine)
(130), (57), (43)
Host Rat
(86), (83), (22), (15), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-6 Receptor antibody is conjugated to PE-Cy7
(22), (14), (11), (10), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(86), (76), (72), (27), (22), (15), (12), (11), (11), (11), (10), (9), (8), (6), (4), (2), (2), (1), (1), (1), (1)
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Product Details anti-IL-6 Receptor Antibody

Target Details IL-6 Receptor Application Details Handling Images
Specificity The monoclonal antibody D7715A7 recognizes mouse CD126, the IL-6 receptor α chain, a member of the Ig superfamily. CD126 can heterodimerize with CD130 (gp130) to form the high affinity IL-6 receptor. Once IL-6 is bound, signaling from the CD130 subunit is initiated and the receptor complex is internalized. The IL-6 receptor is also reported to exist as a soluble version, which bound to IL-6 acts in trans to signal. Expression is found on T lymphocytes in the periphery and during development in the thymus is restricted to SP populations, unlike gp130 which is expressed in all thymocytes. Additional expression has been shown on activated B lymphocytes, macrophages, granulocytes and CD34+ hematopoietic progenitor cells. The monoclonal antibody D7715A7 binding can be blocked addition of ligand (IL-6).
Characteristics The monoclonal antibody D7715A7 recognizes mouse CD126, the IL-6 receptor α chain, a member of the Ig superfamily. CD126 can heterodimerize with CD130 (gp130) to form the high affinity IL-6 receptor. Once IL-6 is bound, signaling from the CD130 subunit is initiated and the receptor complex is internalized. The IL-6 receptor is also reported to exist as a soluble version, which bound to IL-6 acts in trans to signal. Expression is found on T lymphocytes in the periphery and during development in the thymus is restricted to SP populations, unlike gp130 which is expressed in all thymocytes. Additional expression has been shown on activated B lymphocytes, macrophages, granulocytes and CD34+ hematopoietic progenitor cells. The monoclonal antibody D7715A7 binding can be blocked addition of ligand (IL-6).
Synonyms: Synonyms: Interleukin-6 Receptor alpha
Clone D7715A7
Isotype IgG2b

Target Details IL-6 Receptor

Product Details anti-IL-6 Receptor Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD126 (IL6R Antibody Abstract)
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Handling Images back to top
Application Notes Applications reported: This D7715A7 antibody has been reported for use in flow cytometric analysis.

Applications tested: This D7715A7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4 °C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore Page 2 of 2 Anti-Mouse CD126 PE-Cyanine7
Restrictions For Research Use only

Handling

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details Images back to top
Concentration 0.2 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL-6 Receptor antibody (Interleukin-6 Receptor)  (PE-Cy7) (ABIN2677439) anti-Interleukin-6 Receptor (IL6R) antibody (PE-Cy7)