You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human IL12 antibody for ELISA

Recommended IL12 Antibody (supplied by: Log in to see )

Antigen
Interleukin 12 (IL12) Antibodies
  • IL-12 p40
  • il12b
  • il12p40.b
  • zgc:152789
  • LOC100217488
  • IL-12B
  • IL12B
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL-12p40
  • interleukin 12Ba
  • interleukin 12B
  • interleukin 12 p40 subunit
  • interleukin 12a
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • il12ba
  • LOC100217488
  • IL-12
  • Il12a
  • IL12A
  • Il12b
  • IL12B
Reactivity
Human
160
68
16
5
3
2
2
2
1
1
1
Host
Mouse
116
46
43
33
2
1
Clonality
Monoclonal
Conjugate
This IL12 antibody is un-conjugated
32
13
9
8
7
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Neutralization (Neut), ELISA
166
154
57
45
27
20
11
11
10
10
8
7
6
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN1177266
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1585605 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
1 ABIN1172115 IHC ELISA WB Biotin Rabbit IgG AA 23-219 Log in to see Polyclonal
1 ABIN2689732 ELISA Biotin Mouse IgG1, kappa Log in to see C8-6 2
1 ABIN3201242 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 23-219 Log in to see
1 ABIN1822031 Func ELISA WB Goat IgG Log in to see Polyclonal
1 ABIN3031445 FACS IHC ELISA WB Rabbit Ig Fraction AA 168-195 Log in to see Polyclonal
1 ABIN1172113 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 23-219 Log in to see Polyclonal
1 ABIN465143 IHC ELISA WB Mouse IgG1 Log in to see
1 ABIN2474925 ELISA WB Biotin Goat IgG Log in to see Polyclonal 3
1 ABIN1045785 IHC ELISA WB HRP Rabbit IgG Subunit beta Log in to see Polyclonal
1 ABIN1045786 IHC ELISA WB FITC Rabbit IgG Subunit beta Log in to see Polyclonal
1 ABIN1045787 IHC ELISA WB Biotin Rabbit IgG Subunit beta Log in to see Polyclonal
1 ABIN1831194 ELISA WB Biotin Goat IgG Log in to see Polyclonal
1 ABIN1983252 IHC ELISA Mouse IgG1 AA 23-19 Log in to see MM0362-7M58
1 ABIN2474924 Func ELISA WB Goat IgG Log in to see Polyclonal 3
1 ABIN2474928 ELISA Biotin Mouse IgG2b Log in to see CC326 4
1 ABIN2474930 Func FACS ELISA Mouse IgG2b Log in to see CC326 4
1 ABIN2474929 ELISpot Func FACS ELISA WB Mouse IgG2a Log in to see CC301 4
1 ABIN1045784 IHC ELISA WB Rabbit IgG Subunit beta Log in to see Polyclonal
1 ABIN465123 ELISA WB Biotin Goat Log in to see Polyclonal

Top referenced anti-IL12 antibody for ELISA

Similar anti-IL12 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 6 Antibodies
Blocking Peptide (BP) 1 Antibodies
ELISA 101 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Functional Studies (Func) 9 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 51 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Neutralization (Neut) 13 Antibodies

General

Antigen Interleukin 12 (IL12) Antibodies
Reactivity Human
(160), (68), (16), (5), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(116), (46), (43), (33), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL12 antibody is un-conjugated
(32), (13), (9), (8), (7), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Neutralization (Neut), ELISA
(166), (154), (57), (45), (27), (20), (11), (11), (10), (10), (8), (7), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-IL12 Antibody

Target Details IL12 Application Details Handling References for anti-IL12 Antibody (ABIN1177266) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of human IL-12 (p70) bioactivity. Neutralization may be measured with a proliferation assay using 200 pg/mL recombinant human IL-12 (p70) (Cat. No. 554613) to stimulate human PBMC as indicator cells (i.e. preincubation of the antibody with recombinant human IL-12 (p70) for 60 minutes).
50% Neutralization (ND50) at 10-300 ng/mL
>95% Neutralization at 1-20 µg/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Purified p40 Subunit of Human IL-12
Clone C8-6
Isotype IgG1, kappa

Target Details IL12

Product Details anti-IL12 Antibody Application Details Handling References for anti-IL12 Antibody (ABIN1177266) Images back to top
Antigen
Alternative Name IL-12 (p40/p70) (IL12 Antibody Abstract)
Background The C8.6 antibody reacts with human IL-12 p40 monomers, homodimers, and p70 heterodimers, but not p35 subunits. The immunogen used to generate the C8.6 hybridoma was the purified p40 subunit of human IL-12. The C8.6 antibody is a neutralizing antibody and has been reported to strongly inhibit three different biological activities of IL-12 (i.e., IFN-gamma induction), mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity. C8.6 has been shown to immunoprecipitate IL-23.
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL12 Antibody Target Details IL12 Handling References for anti-IL12 Antibody (ABIN1177266) Images back to top
Application Notes ELISA (IL-12 p70 Detection): The biotinylated C8.6 (Cat. No. 554660) antibody is useful as a detection antibody in sandwich ELISA for measuring human IL-12 (p70). Biotinylated C8.6 antibody can be paired with the purified 20C2 antibody (Cat. No. 555065) as the capture antibody and recombinant human IL-12 (p70) protein (Cat. No. 554613) as the standard. For testing human IL-12 (p70) in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Human IL-12 (p70) ELISA Set (Cat. No. 555183). ELISA (IL-12 p40 Detection): The biotinylated C8.6 (Cat. No. 554660) antibody is useful as a detection antibody in sandwich ELISA for measuring human IL-12 (p40). Biotinylated C8.6 antibody can be paired with the purified C8.3 antibody (Cat. No. 551227) as the capture antibody with recombinant human IL-12 (p40) protein (Cat. No. 554633) as the standard. For testing human IL-12 (p40) in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Human IL-12 (p40) ELISA Set (Cat. No. 555171).
Restrictions For Research Use only

Handling

Product Details anti-IL12 Antibody Target Details IL12 Application Details References for anti-IL12 Antibody (ABIN1177266) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL12 Antibody (ABIN1177266)

Product Details anti-IL12 Antibody Target Details IL12 Application Details Handling Images back to top
Product cited in:

Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." in: Immunity, Vol. 13, Issue 5, pp. 715-25, 2001

DAndrea, Aste-Amezaga, Valiante, Ma, Kubin, Trinchieri: "Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells." in: The Journal of experimental medicine, Vol. 178, Issue 3, pp. 1041-8, 1993

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, 1992