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anti-Human IL12 antibody for Neutralization

Recommended IL12 Antibody (supplied by: Log in to see )

Antigen
Interleukin 12 (IL12) Antibodies
  • CLMF
  • CLMF2
  • IL-12 p40
  • Il-12a
  • IL-12B
  • IL-12p35
  • IL-12p40
  • Il12
  • il12b
  • IL12B
  • il12p40.b
  • Ll12a
  • LOC100217488
  • NKSF
  • NKSF2
  • p35
  • zgc:152789
Reactivity
Human
120
60
16
5
3
2
2
2
1
1
1
Host
Mouse
76
46
41
34
2
1
Clonality
Monoclonal
Conjugate
This IL12 antibody is un-conjugated
30
11
8
7
6
4
1
1
1
1
1
1
1
1
1
1
1
1
Application
Neutralization (Neut), ELISA
125
114
43
31
25
21
11
10
9
8
7
7
7
6
5
4
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1177266
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000875249 ABIN223544 Neut WB Goat IgG Log in to see Polyclonal
0.000875249 ABIN282531 ELISpot Neut ELISA Mouse IgG1 kappa Log in to see C8-6
0.000875249 ABIN465439 Neut ELISA WB Goat Log in to see Polyclonal
0.000875249 ABIN2287554 Neut ELISA WB Alkaline Phosphatase (AP) Goat IgG C-Term Log in to see Polyclonal
0.000875249 ABIN2287556 Neut ELISA WB APC Goat IgG C-Term Log in to see Polyclonal
0.000875249 ABIN2287558 Neut ELISA WB Biotin Goat IgG C-Term Log in to see Polyclonal
0.000875249 ABIN2287560 Neut ELISA WB FITC Goat IgG C-Term Log in to see Polyclonal
0.000875249 ABIN2287561 Neut ELISA WB HRP Goat IgG C-Term Log in to see Polyclonal
0.000875249 ABIN2287563 Neut ELISA WB PE Goat IgG C-Term Log in to see Polyclonal
0.000875249 ABIN2287615 Neut ELISA WB Goat IgG C-Term Log in to see Polyclonal
0.000875249 ABIN2287624 Neut ELISA Mouse IgG1 Log in to see 5J87
0.000875249 ABIN281652 FACS ICS Neut WB Mouse IgG1 kappa Log in to see C8-6
0.000875249 ABIN2748959 ELISpot Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.000875249 ABIN2748961 ELISpot Neut ELISA Mouse IgG1 Log in to see QS-12p70

Top referenced anti-IL12 antibody for Neutralization

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Neutralization (Neut) 15 Antibodies

General

Antigen Interleukin 12 (IL12) Antibodies
Reactivity Human
(120), (60), (16), (5), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(76), (46), (41), (34), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL12 antibody is un-conjugated
(30), (11), (8), (7), (6), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Neutralization (Neut), ELISA
(125), (114), (43), (31), (25), (21), (11), (10), (9), (8), (7), (7), (7), (6), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL12 Antibody

Target Details IL12 Application Details Handling References for anti-IL12 Antibody (ABIN1177266) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of human IL-12 (p70) bioactivity. Neutralization may be measured with a proliferation assay using 200 pg/mL recombinant human IL-12 (p70) (Cat. No. 554613) to stimulate human PBMC as indicator cells (i.e. preincubation of the antibody with recombinant human IL-12 (p70) for 60 minutes).
50% Neutralization (ND50) at 10-300 ng/mL
>95% Neutralization at 1-20 µg/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Purified p40 Subunit of Human IL-12
Clone C8-6
Isotype IgG1, kappa

Target Details IL12

Product Details anti-IL12 Antibody Application Details Handling References for anti-IL12 Antibody (ABIN1177266) Images back to top
Antigen
Alternative Name IL-12 (p40/p70) (IL12 Antibody Abstract)
Background The C8.6 antibody reacts with human IL-12 p40 monomers, homodimers, and p70 heterodimers, but not p35 subunits. The immunogen used to generate the C8.6 hybridoma was the purified p40 subunit of human IL-12. The C8.6 antibody is a neutralizing antibody and has been reported to strongly inhibit three different biological activities of IL-12 (i.e., IFN-gamma induction), mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity. C8.6 has been shown to immunoprecipitate IL-23.
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL12 Antibody Target Details IL12 Handling References for anti-IL12 Antibody (ABIN1177266) Images back to top
Application Notes ELISA (IL-12 p70 Detection): The biotinylated C8.6 (Cat. No. 554660) antibody is useful as a detection antibody in sandwich ELISA for measuring human IL-12 (p70). Biotinylated C8.6 antibody can be paired with the purified 20C2 antibody (Cat. No. 555065) as the capture antibody and recombinant human IL-12 (p70) protein (Cat. No. 554613) as the standard. For testing human IL-12 (p70) in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Human IL-12 (p70) ELISA Set (Cat. No. 555183). ELISA (IL-12 p40 Detection): The biotinylated C8.6 (Cat. No. 554660) antibody is useful as a detection antibody in sandwich ELISA for measuring human IL-12 (p40). Biotinylated C8.6 antibody can be paired with the purified C8.3 antibody (Cat. No. 551227) as the capture antibody with recombinant human IL-12 (p40) protein (Cat. No. 554633) as the standard. For testing human IL-12 (p40) in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Human IL-12 (p40) ELISA Set (Cat. No. 555171).
Restrictions For Research Use only

Handling

Product Details anti-IL12 Antibody Target Details IL12 Application Details References for anti-IL12 Antibody (ABIN1177266) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL12 Antibody (ABIN1177266)

Product Details anti-IL12 Antibody Target Details IL12 Application Details Handling Images back to top
Product cited in:

Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." in: Immunity, Vol. 13, Issue 5, pp. 715-25, 2001

DAndrea, Aste-Amezaga, Valiante, Ma, Kubin, Trinchieri: "Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells." in: The Journal of experimental medicine, Vol. 178, Issue 3, pp. 1041-8, 1993

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, 1992