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anti-Mouse (Murine) IL12 antibody for ELISA

Recommended IL12 Antibody (supplied by: Log in to see )

Antigen
Interleukin 12 (IL12) Antibodies
  • IL-12 p40
  • il12b
  • il12p40.b
  • zgc:152789
  • LOC100217488
  • IL-12B
  • IL12B
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL-12p40
  • interleukin 12Ba
  • interleukin 12B
  • interleukin 12 p40 subunit
  • interleukin 12a
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • il12ba
  • LOC100217488
  • IL-12
  • Il12a
  • IL12A
  • Il12b
  • IL12B
Reactivity
Mouse (Murine)
161
61
16
9
5
2
2
2
1
1
1
Host
Rat
111
44
44
32
2
1
Clonality
Monoclonal
Conjugate
This IL12 antibody is un-conjugated
32
13
8
8
7
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Neutralization (Neut), ELISA, Western Blotting (WB)
165
153
66
41
28
19
14
13
11
7
6
6
5
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN1177291
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN269610 ELISA IHC IHC (p) WB Goat Log in to see Polyclonal 2
1 ABIN1585605 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
1 ABIN2689728 IP ELISA WB Biotin Rat IgG2a Log in to see C17-8 3
1 ABIN2689730 ELISA Rat IgG1 Log in to see C15-6 3
1 ABIN465114 Neut ELISA WB Goat Log in to see Polyclonal
1 ABIN4324478 BP ELISA Neut WB Rabbit IgG Log in to see Polyclonal
1 ABIN1002588 Inhibition ELISA WB Goat IgG Log in to see Polyclonal
1 ABIN1825606 ELISA Rat IgG2b Log in to see NYRmIL-12p75
1 ABIN1002589 ELISA Rat IgG2b Log in to see NYRmIL-12p75
1 ABIN2850722 ELISA WB FITC Rat IgG1 kappa Log in to see 14G16
1 ABIN2850672 ELISA WB Biotin Rat IgG1 kappa Log in to see 14G16
1 ABIN2850822 ELISA WB PE Rat IgG1 kappa Log in to see 14G16
1 ABIN2287547 ELISA WB Rat IgG1 kappa Log in to see 14G16
1 ABIN2850772 ELISA WB HRP Rat IgG1 kappa Log in to see 14G16
1 ABIN2850573 ELISA WB Alkaline Phosphatase (AP) Rat IgG1 kappa Log in to see 14G16
1 ABIN2850622 ELISA WB APC Rat IgG1 kappa Log in to see 14G16
1 ABIN933784 Inhibition ELISA WB Rat IgG1 kappa Log in to see M912296
1 ABIN1002586 Inhibition ELISA Rat IgG1 kappa Log in to see
1 ABIN465132 ELISA WB Rat IgG1 Log in to see
1 ABIN1967956 IP ELISA WB Mouse IgG2b Log in to see NYRmIL-12p75

Top referenced anti-IL12 antibody for ELISA

Similar anti-IL12 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 5 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cellular Assay (CA) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA 50 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Immunoprecipitation (IP) 12 Antibodies
Immunostaining (ISt) 2 Antibodies

General

Antigen Interleukin 12 (IL12) Antibodies
Reactivity Mouse (Murine)
(161), (61), (16), (9), (5), (2), (2), (2), (1), (1), (1)
Host Rat
(111), (44), (44), (32), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL12 antibody is un-conjugated
(32), (13), (8), (8), (7), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Neutralization (Neut), ELISA, Western Blotting (WB)
(165), (153), (66), (41), (28), (19), (14), (13), (11), (7), (6), (6), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-IL12 Antibody

Target Details IL12 Application Details Handling References for anti-IL12 Antibody (ABIN1177291) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neturalization of recombinant mouse IL-12 (p70). Neutralization activity may be measured with a proliferation assay using 300 pg/mL recombinant mouse IL-12 (p70) (Cat. No. 554592) to stimulate 2D6 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-12 (p70) for 60 minutes).
50% Neutralization (ND50) at 4 - 100 ng/mL
>95% Neutralization at 60 - 600 ng/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Recombinant Mouse IL-12 p70
Clone C17-8
Isotype IgG2a

Target Details IL12

Product Details anti-IL12 Antibody Application Details Handling References for anti-IL12 Antibody (ABIN1177291) Images back to top
Antigen
Alternative Name IL-12 (p40/p70) (IL12 Antibody Abstract)
Background The C17.8 antibody reacts with the free p40 monomer of IL-12 as well as the p40 subunit complexed as homodimer or complexed with p35 as a p70 heterodimer. Clone C17.8 has also been shown to be cross-reactive with mouse IL-23, which also contains the p40 subunit. The immunogen used to generate C17.8 hybridoma was recombinant mouse IL-12 p70.
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-IL12 Antibody Target Details IL12 Handling References for anti-IL12 Antibody (ABIN1177291) Images back to top
Application Notes ELISA (IL-12 p70 Detection): The biotinylated C17.8 antibody (Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA for specifically measuring mouse IL-12 p70 protein levels. Biotinylated C17.8 antibody can be paired with the purified 9A5 (Cat. No. 554658) as the capture antibody and with recombinant mouse IL-12 p70 (Cat. No.554592) as the standard. For testing mouse IL-12 p70 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the Mouse IL-12 (p70) BD OptEIA™ ELISA Set (Cat. No. 555256).
ELISA (IL-12 p40 Detection): The biotinylated C17.8 antibody (Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA measuring IL-12 p40 protein levels (free p40 monomer as well as p40 complexes as homodimer or heterodimer). Biotinylated C17.8 antibody can be paired with the purified C15.6 antibody (Cat. No. 551219) as the capture antibody, and with recombinant mouse IL-12 p70 protein (Cat. No.554592) or IL-12 p40 protein (Cat. No. 554594) as the standard. For testing mouse IL-12 p40 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the Mouse IL-12 (p40) BD OptEIA™ ELISA Set (Cat. No. 555165).
Restrictions For Research Use only

Handling

Product Details anti-IL12 Antibody Target Details IL12 Application Details References for anti-IL12 Antibody (ABIN1177291) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL12 Antibody (ABIN1177291)

Product Details anti-IL12 Antibody Target Details IL12 Application Details Handling Images back to top
Product cited in:

Maruo, Ahn, Yu, Tomura, Wysocka, Yamamoto, Kobayashi, Hamaoka, Trinchieri, Fuijiwara: "Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity." in: Journal of leukocyte biology, Vol. 61, Issue 3, pp. 346-52, 1997

Neurath, Fuss, Kelsall, Stüber, Strober: "Antibodies to interleukin 12 abrogate established experimental colitis in mice." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1281-90, 1995

Wysocka, Kubin, Vieira, Ozmen, Garotta, Scott, Trinchieri: "Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice." in: European journal of immunology, Vol. 25, Issue 3, pp. 672-6, 1995

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, 1992