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anti-Mouse (Murine) IL12 antibody for Flow Cytometry

Recommended IL12 Antibody (supplied by: Log in to see )

Antigen
Interleukin 12 (IL12) Antibodies
  • IL-12 p40
  • il12b
  • il12p40.b
  • zgc:152789
  • LOC100217488
  • IL-12B
  • IL12B
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL-12p40
  • interleukin 12Ba
  • interleukin 12B
  • interleukin 12 p40 subunit
  • interleukin 12a
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • il12ba
  • LOC100217488
  • IL-12
  • Il12a
  • IL12A
  • Il12b
  • IL12B
Epitope
AA 23-219
17
15
14
7
5
3
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
162
67
16
5
3
2
2
2
1
1
1
Host
Mouse
116
46
45
33
2
1
Clonality
Monoclonal
Conjugate
This IL12 antibody is conjugated to Allophycocyanin
32
13
9
8
7
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
163
154
61
44
28
20
14
13
11
7
6
6
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4896563
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.113617 ABIN4896566 FACS PE Mouse IgG1 AA 23-219 Log in to see 27537 3
13.113617 ABIN4896565 FACS Fluorescein Mouse IgG1 AA 23-219 Log in to see 27537 5
13.113617 ABIN4896564 FACS PerCP Mouse IgG1 AA 23-219 Log in to see 27537
1 ABIN2665104 Func FACS IHC WB Rat IgG2a kappa Log in to see C18-2 5
1 ABIN4899729 FACS WB Mouse IgG1 AA 23-219 Log in to see 27537 10
1 ABIN4899730 FACS WB Mouse IgG1 AA 23-219 Log in to see 27537 10
1 ABIN2665103 Func FACS IHC WB Rat IgG2a kappa Log in to see C18-2 5
1 ABIN438595 CyTOF FACS IP WB Rat IgG1 Log in to see AP-MAB0853
1 ABIN4324477 CyTOF FACS IP WB Rat IgG1 Log in to see AP-MAB0853
1 ABIN2287565 IC FACS IP ELISA WB Rat IgG1 Log in to see 5J86

Top referenced anti-IL12 antibody for Flow Cytometry

Similar anti-IL12 Antibodies

Application / Reactivity Mouse (Murine) Human
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Western Blotting (WB) 52 Antibodies 95 Antibodies
Transfected Cell Culture (TCC) 2 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Neutralization (Neut) 14 Antibodies 13 Antibodies
Immunostaining (ISt) 2 Antibodies
Immunoprecipitation (IP) 1 Antibodies 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 52 Antibodies 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies

General

Antigen Interleukin 12 (IL12) Antibodies
Epitope AA 23-219
(17), (15), (14), (7), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(162), (67), (16), (5), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(116), (46), (45), (33), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL12 antibody is conjugated to Allophycocyanin
(32), (13), (9), (8), (7), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(163), (154), (61), (44), (28), (20), (14), (13), (11), (7), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL12 Antibody

Target Details IL12 Application Details Handling References for anti-IL12 Antibody (ABIN4896563) Images
Specificity Detects human IL-12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Purification Protein A or G purified from hybridoma culture supernatant
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12/IL-35 p35, Arg23-Ser219, Accession # P29459
Clone 27537
Isotype IgG1

Target Details IL12

Product Details anti-IL12 Antibody Application Details Handling References for anti-IL12 Antibody (ABIN4896563) Images back to top
Antigen
Alternative Name IL-12 (IL12 Antibody Abstract)
UniProt P29459
Research Area Virology, Immunology, Cytokines, Cancer, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL12 Antibody Target Details IL12 Handling References for anti-IL12 Antibody (ABIN4896563) Images back to top
Application Notes Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Restrictions For Research Use only

Handling

Product Details anti-IL12 Antibody Target Details IL12 Application Details References for anti-IL12 Antibody (ABIN4896563) Images back to top
Format Liquid
Buffer Supplied in a saline solution containing BSA and Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Expiry Date 12 months

References for anti-IL12 Antibody (ABIN4896563)

Product Details anti-IL12 Antibody Target Details IL12 Application Details Handling Images back to top
Product cited in:

Wang, Shi, Ma, Ji, Ying, Wu, Wang, Li, Moorman, Yao: "Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection." in: Journal of virology, Vol. 87, Issue 8, pp. 4372-83, 2013 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

van Herwijnen, Wieten, van der Zee, van Kooten, Wagenaar-Hilbers, Hoek, den Braber, Anderton, Singh, Meiring, van Els, van Eden, Broere: "Regulatory T cells that recognize a ubiquitous stress-inducible self-antigen are long-lived suppressors of autoimmune arthritis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 35, pp. 14134-9, 2012 Method employed by authors: Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Images

Product Details anti-IL12 Antibody Target Details IL12 Application Details Handling References for anti-IL12 Antibody (ABIN4896563) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL12 antibody (Interleukin 12) (AA 23-219) (Allophycocyanin) (ABIN4896563) Detection of IL-12/IL-35 p35 in Human PBMCs or Mouse Splenocytes by Flow Cytometry. (...