anti-Mouse (Murine) IL12 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended IL12 Antibody (supplied by: Log in to see )

Antigen
Interleukin 12 (IL12) Antibodies
  • IL-12 p40
  • il12b
  • il12p40.b
  • zgc:152789
  • LOC100217488
  • IL-12B
  • IL12B
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL-12p40
  • interleukin 12Ba
  • interleukin 12B
  • interleukin 12 p40 subunit
  • interleukin 12a
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • il12ba
  • LOC100217488
  • IL-12
  • Il12a
  • IL12A
  • Il12b
  • IL12B
Reactivity
Mouse (Murine)
175
74
16
9
5
2
2
2
1
1
1
Host
Goat
125
46
44
43
2
1
Clonality
Polyclonal
Conjugate
This IL12 antibody is un-conjugated
34
14
10
8
7
5
5
4
4
4
4
4
4
4
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
166
165
79
41
29
19
18
15
13
11
7
6
5
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN269610
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General

Antigen Interleukin 12 (IL12) Antibodies
Reactivity Mouse (Murine)
(175), (74), (16), (9), (5), (2), (2), (2), (1), (1), (1)
Host Goat
(125), (46), (44), (43), (2), (1)
Clonality
Polyclonal
Conjugate This IL12 antibody is un-conjugated
(34), (14), (10), (8), (7), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(166), (165), (79), (41), (29), (19), (18), (15), (13), (11), (7), (6), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL12 Antibody

Target Details IL12 Application Details Handling ProductDetails: References for anti-IL12 antibody (ABIN269610) Images
Specificity Based on ELISA, the antibody shows ~10 % cross-reactivity with recombinant human IL-12 and <5 % cross-reactivity with recombinant porcine IL-12.
Purification Protein G purified
Sterility 0.2 μm filtered
Immunogen Purified, insect cell line Sf21-derived, recombinant mouse IL-12

Target Details IL12

Product Details anti-IL12 Antibody Application Details Handling ProductDetails: References for anti-IL12 antibody (ABIN269610) Images back to top
Antigen
Alternative Name IL-12 (IL12 Antibody Abstract)
Background Gene Symbol: IL12A
Gene ID 3592
UniProt P43431
Research Area Immunology, Cytokines, Inflammation, Virology, Cancer
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-IL12 Antibody Target Details IL12 Handling ProductDetails: References for anti-IL12 antibody (ABIN269610) Images back to top
Application Notes Western Blot 1-2 μg/mL, ELISA 0.5-1 μg/mL, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500ELISA: Sensitivity = 0.2 ng/well Neut: Achieving half of the neutralizing dose of a 0.3 ng/mL concentration of Mouse IL12 requires 0.012-0.2 μg/mL. WB: Predicted molecular weight: 39.4 kDa. Sensitivity = 1.5-3.0 ng/lane. Use in Immunohistochemistry on paraffin sections was reported in a scientific publication (PMID: 23666011).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-IL12 Antibody Target Details IL12 Application Details ProductDetails: References for anti-IL12 antibody (ABIN269610) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.2μm filtered solution in PBS and 5 % Trehalose
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-IL12 antibody (ABIN269610)

Product Details anti-IL12 Antibody Target Details IL12 Application Details Handling Images back to top
Product cited in:

Lee, Lee, Kim, Kim, Yang, Jeong, Seong: "Radiation Inhibits Interleukin-12 Production via Inhibition of C-Rel through the Interleukin-6/ Signal Transducer and Activator of Transcription 3 Signaling Pathway in Dendritic Cells." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146463, 2016

Zhang, Cao, Bai, Carr, Ella-Menye, Irvin, Ratner, Jiang: "Zwitterionic hydrogels implanted in mice resist the foreign-body reaction." in: Nature biotechnology, Vol. 31, Issue 6, pp. 553-6, 2013 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)