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Mouse (Murine) IL12 ELISA Kit

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Antigen
Interleukin 12 (IL12) ELISA Kits
  • CLMF
  • CLMF2
  • IL-12 p40
  • Il-12a
  • IL-12B
  • IL-12p35
  • IL-12p40
  • Il12
  • il12b
  • IL12B
  • il12p40.b
  • Ll12a
  • LOC100217488
  • NKSF
  • NKSF2
  • p35
  • zgc:152789
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
68
33
10
9
8
7
6
5
5
4
4
4
1
1
Methode Type
Sandwich ELISA
Detection Range
16-1000 pg/mL
Minimum Detection Limit
16 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN924811
$ 380.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.3274747 ABIN2472094 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.3274747 ABIN1115607 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.63 pg/mL Log in to see
0.3274747 ABIN2708537 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.3274747 ABIN2708538 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.3274747 ABIN2762261 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 0.02-1.0 ng/mL 0.02-1.0 ng/mL Log in to see
0.3274747 ABIN2064458 Cell Culture Supernatant, Serum, Plasma Log in to see
0.3274747 ABIN2114261 Cell Culture Supernatant, Serum Log in to see
0.0012092739 ABIN365243 Sandwich ELISA Serum, Plasma, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012092739 ABIN628002 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012092739 ABIN628038 Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids Log in to see
0.0012092739 ABIN2703167 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 5-2000 pg/mL 5 pg/mL Log in to see
0.0012092739 ABIN1112723 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012092739 ABIN2114259 Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.0012092739 ABIN2748838 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012092739 ABIN1305264 Log in to see 6
0.0012092739 ABIN2042522 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012092739 ABIN1033586 Sandwich ELISA Log in to see
0.0012092739 ABIN2684572 Sandwich ELISA Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012092739 ABIN2762322 Serum, Plasma, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012092739 ABIN2116069 0.15-150 μg/mL 0.15 μg/mL Log in to see

Top referenced IL12 ELISA Kit for ELISA

  • Mouse (Murine) IL12 ELISA Kit - ABIN1305264 (6 Publications): Nguyen, Duncan, Mirtsos, Ng, Speiser, Shahinian, Marino, Mak, Ohashi, Yeh: TRAF2 deficiency results in hyperactivity of certain TNFR1 signals and impairment of CD40-mediated responses. in Immunity 1999 (PubMed)

Similar IL12 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 33 ELISA Kits

General

Antigen Interleukin 12 (IL12) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(68), (33), (10), (9), (8), (7), (6), (5), (5), (4), (4), (4), (1), (1)
Methode Type Sandwich ELISA
Detection Range 16-1000 pg/mL
Minimum Detection Limit 16 pg/mL
Application ELISA
Supplier Log in to see

Product Details IL12 ELISA Kit

Target details Application Details Handling Images
Purpose The OmniKine? Murine IL-12 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine IL-12 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine IL-12 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non- specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Brand OmniKine™
Sample Type Cell Lysate, Serum, Plasma
Detection Method Colorimetric
Specificity The Murine IL-12 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine IL-12 proteins.
Cross-Reactivity (Details) The Murine IL-12 ELISA is capable of recognizing both recombinant and naturally produced Murine IL-12 proteins. The antigens listed below were tested at 50 ng/mL and exhibited 100% cross reactivity. Murine: IL-12p40, IL-12p80 The antigens listed below were tested at 50 ng/mL and exhibited less than 1% cross reactivity. Human: IL-12p40, IL-12p70, IL-12p80 The antigens listed below were tested at 50 ng/mL and did not exhibit significant cross reactivity or interference. Human: IFN-γ, IL-10 Murine: IFN-γ, IL-10
Characteristics The Murine IL-12 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine IL-12 proteins within the range of 16-1000 pg/mL.
Components
  • Microstrips Coated w / Capture Antibody: 12 x 8-Well Microstrips
  • Protein Standard: Lyophilized (100 ng), Red container
  • Biotinylated Detection Antibody: Lyophilized, Yellow container
  • 400x Streptavidin-HRP: 30 μL, Blue container
  • Wash Buffer (10x): 50 mL, Clear containter
  • Assay Diluent: 50 mL, Clear container
  • Ready-to-Use Substrate: 12 mL, Brown container
  • Stop Solution: 12 mL, Clear container
  • Adhesive Plate Sealers: 4 Sheets
  • Technical Manual 1 Manual
Material not included The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:
Microplate reader able to measure absorbance at 450 nm (with correction wavelength set to 540 nm or 570 nm)
Micropipettes with capability of measuring volumes ranging from 1 μl to 1 mL
Deionized or sterile water
Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
Graph paper or computer software capable of generating or displaying logarithmic functions
Absorbent paper or vacuum aspirator
Test tubes or microfuge tubes capable of storing ≥1 mL
Bench
top centrifuge (optional)
Bench
top vortex (optional)
Orbital shaker (optional)

Target details

Product Details IL12 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name IL-12 (IL12 ELISA Kit Abstract)
Background Murine IL-12, also known as Interleukin-12 or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimer composed of the mIL-12A and mIL-12B subunits that are 215 and 335 amino acid residues expressed from chromosome 3 and 11, respectively. IL-12A, also known as CLMF p35, is a cytokine that can act as a growth factor for activated T- and NK cells, enhance the activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-γ by resting PBMC. However, the IL-12B subunit possesses the exact same properties, except has the ability to associate with IL-23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. It also binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. The IL-12B subunit belongs to the type I cytokine receptor family type 3 subfamily and contains 1 fibronectin type-III domain as well as 1 Ig-like C2-type domain. Source: Entrez Gene: Il12a interleukin 12a [Mus musculus], Il12b interleukin 12b [Mus musculus], Swiss-Prot: P43431, P43432
Gene ID 16159, 16160
UniProt P43431, P43432
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details IL12 ELISA Kit Target details Handling Images back to top
Plate Strips (12 x 8)
Protocol This particular immunoassay utilizes the quantitative technique of a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a sandwich format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Murine IL-12 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and sandwiching of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’- Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Sample Preparation If samples are to be used within 24 hours, aliquot and store at 4 °C. If samples are to be used over a long period of time, aliquot and store between -20 °C and -80 °C, depending on the duration of storage.
Note: Samples containing a visible precipitate or pellet must be clarified prior to use in the assay.
Caution: Avoid repeated freeze/thaw cycles to prevent loss of biological activity of proteins in experimental samples.
  • Cell Lysate and Supernatants:
    Remove large cell components via centrifugation and perform the assay. Cell lysates and supernatants require a dilution using Assay Diluent. A serial dilution may be performed to determine a suitable dilution factor for the sample. For future use of the sample, follow the sample storage guidelines stated above.
  • Serum:
    Allow samples to clot in a serum separator tube (SST) for 30 minutes. After sufficient clotting, centrifuge at 1000 x g for 15 minutes and remove serum from SST in preparation for the assay. Serum samples require at least a 1:50 dilution using Assay Diluent. For future use of the sample, follow the storage guidelines above.
  • Plasma:
    Use heparin, citrate or EDTA as an anticoagulant to gather plasma from original biological sample. After collection of the plasma, centrifuge for 15 minutes at 1000 x g. This step must be performed within 30 minutes of plasma collection. Plasma samples require at least a 1:50 dilution using Assay Diluent. Afterwards, perform the assay or for future use of the sample, follow the storage guidelines stated above.
Assay Procedure Note: If possible, all incubation steps should be performed on an orbital shaker to equilibrate solutions when added to the microplate wells. Also, all provided solutions should be at ambient temperature prior to use.
Note: Avoid adding solutions into wells at an angle, always keep pipette tip perpendicular to plate bottom.

Reconstitution of Provided Materials:
    1. Reconstitute the Biotin-Conjugated Detection Antibody in 67 µL of ddH₂O for a concentration of 180 µg/ml.
    2. Reconstitute the Protein Standard in 100 µL of ddH₂O for a concentration of 340 ng/ml.
    3. Dilute the 50 mL of 10x Wash Buffer in 450 mL of ddH2O for 500 mL of 1x Wash Buffer.
Addition of Known Standard and Unknown Sample to Immunoassay:
    The OmniKine™ Human CD163 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD163 proteins
Calculation of Results Generation of Standard Curve and Interpretation of Data
1. Average the duplicate or triplicate readings for each standard, control and sample and subtract the average zero standard optical density.
2. Generate a standard curve by using Microsoft Excel or other computer software capable of establishing a 4- Parameter Logistic (4-PL) curve fit. If using Excel or an alternative graphing tool, plot the average optical density values in absorbance units (y-axis) against the known standard concentrations in pg/ml (x-axis). Note: Only use the values in which a noticeable gradient can be established. Afterwards, generate a best fit curve or trend-line through the plotted points via regression analysis.
Restrictions For Research Use only

Handling

Product Details IL12 ELISA Kit Target details Application Details Images back to top
Precaution of Use Reagents provided in this kit may be harmful if ingested, inhaled or absorbed through the skin. Please carefully review the MSDS for each reagent before conducting the experiment.
Stop Solution contains 2 N Sulfuric Acid (H2SO4) and is an extremely corrosive agent. Please wear proper eye, hand and face protection when handling this material. When the experiment is finished, be sure to rinse the plate with copious amounts of running water to dilute the Stop Solution prior to disposing the plate.
Handling Advice This ELISA kit is intended for research purposes only, NOT diagnostic or clinical procedures of any kind.
Materials included in this kit should NOT be used past the expiration date on the kit label.
Reagents or substrates included in this kit should NOT be mixed or substituted with reagents or substrates from any other kits.
Variations in pipetting technique, washing technique, operator laboratory technique, kit age, incubation time or temperature may cause differences in binding affinity of the materials provided.
The assay is designed to eliminate interference and background by other cellular macromolecules or factors present within any biological samples. However, the possibility of background noise cannot be fully excluded until all factors have been tested using the assay kit.

Reagents provided in this kit may be harmful if ingested, inhaled or absorbed through the skin. Please carefully review the MSDS for each reagent before conducting the experiment.
Stop Solution contains 2 N Sulfuric Acid (H2SO4) and is an extremely corrosive agent. Please wear proper eye, hand and face protection when handling this material. When the experiment is finished, be sure to rinse the plate with copious amounts of running water to dilute the Stop Solution prior to disposing the plate.
Storage 4 °C
Storage Comment Note: If used frequently, reagents may be stored at 4 °C.
  • Unopened Kits: Store at 4 °C for 6 months.
  • Microstrips Coated w/ Capture Antibody, 400x Streptavidin-HRP Wash Buffer (10x), Assay Diluent Ready-to-Use Substrate, Stop Solution: 6 Months at 4 °C
  • Protein Standard, Biotinylated Detection Antibody: Lyophilized: 6 Months (if Reconstituted: 1 Month) at 4 °C

Images

Product Details IL12 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for IL12 ELISA Kit (Interleukin 12) (ABIN924811) This is an example of what a typical standard curve will look like. You must make you...