anti-Cow (Bovine) IL12B antibody for Enzyme Immunoassay

Recommended IL12B Antibody (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) Antibodies
  • IL-12B
  • IL12B
  • p40
  • Il-12b
  • Il12p40
  • Il-12p40
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL12p40
  • IL-12
  • IL-12.p40
  • IL-12p40
  • interleukin 12 p40 subunit
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12b
  • interleukin 12B
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • IL-12
  • IL12A
  • Il12b
  • IL12B
Reactivity
Cow (Bovine), Human, Sheep (Ovine)
150
63
28
24
22
3
2
2
1
1
1
Host
Mouse
137
58
34
11
4
1
1
Clonality
Monoclonal
Conjugate
This IL12B antibody is un-conjugated
20
16
15
8
6
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS)
169
154
64
25
22
18
12
6
6
4
4
3
2
2
1
1
1
1
1
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Catalog No. ABIN317391
$ 753.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1107794 ELISpot Func EIA FACS WB Mouse IgG2a Log in to see CC301

Similar anti-IL12B Antibodies

Application / Reactivity Sheep (Ovine) Human
ELISA 15 Antibodies 94 Antibodies
ELISpot 1 Antibodies 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 5 Antibodies
Flow Cytometry (FACS) 2 Antibodies 26 Antibodies
Functional Studies (Func) 2 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Western Blotting (WB) 16 Antibodies
Cell Culture (CC) 1 Antibodies
Dot Blot (DB) 3 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
Immunoassay (IA) 2 Antibodies
Immunofluorescence (IF) 1 Antibodies

General

Antigen Interleukin 12b (IL12B) Antibodies
Reactivity Cow (Bovine), Human, Sheep (Ovine)
(150), (63), (28), (24), (22), (3), (2), (2), (1), (1), (1)
Host Mouse
(137), (58), (34), (11), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL12B antibody is un-conjugated
(20), (16), (15), (8), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS)
(169), (154), (64), (25), (22), (18), (12), (6), (6), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-IL12B Antibody

Target Details IL12B Application Details Handling Images
Specificity This antibody recognizes Interleukin-12. It has been shown to block the biological activity of IL-12.
Characteristics Synonyms: Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2, Cytotoxic lymphocytematuration factor 40 kDa subunit, NK cell stimulatory factor chain 2
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Recombinant bovine IL-12
Clone CC326
Isotype IgG2b

Target Details IL12B

Product Details anti-IL12B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-12 beta / IL12B (IL12B Antibody Abstract)
Background Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2
Gene ID 281857
UniProt P46282
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-IL12B Antibody Target Details IL12B Handling Images back to top
Application Notes ELISA: 1/100 - 1/1000. Flow cytometry: 1/1000, use 10 μL to label 10^6 cells of 100 l. Functional assays: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL12B Antibody Target Details IL12B Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, None, None
Preservative Azide free
Storage -20 °C
Storage Comment Store the antibody undiluted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months