anti-Human IL12B antibody for Flow Cytometry

Recommended IL12B Antibody (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) Antibodies
  • IL-12B
  • IL12B
  • p40
  • Il-12b
  • Il12p40
  • Il-12p40
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL12p40
  • IL-12
  • IL-12.p40
  • IL-12p40
  • interleukin 12 p40 subunit
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12b
  • interleukin 12B
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • IL-12
  • IL12A
  • Il12b
  • IL12B
Epitope
AA 271-298, C-Term
23
19
12
11
11
11
7
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human
152
63
28
25
22
4
2
2
1
1
1
Host
Rabbit
138
60
34
10
4
1
1
Clonality
Polyclonal
Conjugate
This IL12B antibody is un-conjugated
20
16
15
8
6
6
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
169
154
64
26
22
17
12
6
6
4
4
3
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN952920
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.052211 ABIN4898082 FACS Fluorescein Mouse IgG2b AA 23-328 Log in to see 169503 1
14.052211 ABIN654420 FACS IHC (p) WB Rabbit Ig AA 264-290, C-Term Log in to see Polyclonal
14.052211 ABIN3031446 FACS IHC ELISA WB Rabbit Ig Fraction AA 264-290 Log in to see Polyclonal
1 ABIN1107790 FACS FITC Mouse IgG1 Log in to see B-P24 1
1 ABIN1107789 Func FACS Mouse IgG1 Log in to see B-P24 1
1 ABIN1107792 FACS Mouse IgG1 Log in to see B-P40 2
1 ABIN473935 FACS PE Mouse IgG1, kappa Log in to see HP40-HP-40
1 ABIN1102450 FACS IHC (p) Neut WB Mouse IgG1, kappa Log in to see C8-6
1 ABIN317391 EIA Func FACS Mouse IgG2b Log in to see CC326
1 ABIN1724135 FACS IHC (p) WB Mouse IgG1 kappa Log in to see C8-6
1 ABIN5644825 FACS Fluorescein Mouse IgG2b AA 23-328 Log in to see 169503
1 ABIN5533081 FACS IHC (p) WB Rabbit Ig Fraction AA 264-290, C-Term Log in to see Polyclonal
1 ABIN2290520 FACS IHC ELISA WB Rabbit IgG C-Term, AA 271-298 Log in to see Polyclonal
1 ABIN1904904 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 271-298 Log in to see Polyclonal
1 ABIN1904906 FACS IHC ELISA WB PE Rabbit IgG AA 271-298 Log in to see Polyclonal
1 ABIN2290538 FACS IHC ELISA WB FITC Rabbit IgG C-Term, AA 271-298 Log in to see Polyclonal
1 ABIN2290534 FACS IHC ELISA WB APC Rabbit IgG C-Term, AA 271-298 Log in to see Polyclonal
1 ABIN1904905 FACS IHC ELISA WB APC Rabbit IgG AA 271-298 Log in to see Polyclonal
1 ABIN2290543 FACS IHC ELISA WB PE Rabbit IgG C-Term, AA 271-298 Log in to see Polyclonal
1 ABIN2609434 ELISA FACS IHC WB Rabbit IgG AA 271-298 Log in to see Polyclonal

General

Antigen Interleukin 12b (IL12B) Antibodies
Epitope AA 271-298, C-Term
(23), (19), (12), (11), (11), (11), (7), (7), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(152), (63), (28), (25), (22), (4), (2), (2), (1), (1), (1)
Host Rabbit
(138), (60), (34), (10), (4), (1), (1)
Clonality
Polyclonal
Conjugate This IL12B antibody is un-conjugated
(20), (16), (15), (8), (6), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(169), (154), (64), (26), (22), (17), (12), (6), (6), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL12B Antibody

Target Details IL12B Application Details Handling References for anti-IL12B antibody (ABIN952920) Images
Specificity This antibody detects human Interleukin-12 beta/IL12Bat C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Purified through a protein A column; followed by peptide affinity purification.
Immunogen Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 271-298aa) of human Interleukin-12 beta/IL12B.
Isotype Ig

Target Details IL12B

Product Details anti-IL12B Antibody Application Details Handling References for anti-IL12B antibody (ABIN952920) Images back to top
Antigen
Alternative Name Interleukin-12 beta / IL12B (IL12B Antibody Abstract)
Background IL12B is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2
Gene ID 3593
NCBI Accession NP_002178
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-IL12B Antibody Target Details IL12B Handling References for anti-IL12B antibody (ABIN952920) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL12B Antibody Target Details IL12B Application Details References for anti-IL12B antibody (ABIN952920) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL12B antibody (ABIN952920)

Product Details anti-IL12B Antibody Target Details IL12B Application Details Handling Images back to top
Background publications

Sobti, Salih, Nega, Seyed, Rupinder, Vijesh, Ajay: "Insights into the role of IL-12B and IFN-gamma cytokine gene polymorphisms in HIV-1/AIDS infection." in: Folia biologica, Vol. 56, Issue 3, pp. 110-5, 2010

Torres de Heens, Loos, van der Velden: "Monozygotic twins are discordant for chronic periodontitis: white blood cell counts and cytokine production after ex vivo stimulation." in: Journal of clinical periodontology, Vol. 37, Issue 2, pp. 129-36, 2010

Images

Product Details anti-IL12B Antibody Target Details IL12B Application Details Handling References for anti-IL12B antibody (ABIN952920) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Interleukin 12b (IL12B) (AA 271-298), (C-Term) antibody (ABIN952920) Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Interleukin 12b (IL12B) (AA 271-298), (C-Term) antibody (ABIN952920) Immunohistochemistry analysis in human tonsil tissue (formalin-fixed, paraffin-embedd...
Western Blotting (WB) image for anti-Interleukin 12b (IL12B) (AA 271-298), (C-Term) antibody (ABIN952920) Western blot analysis in CEM,MDA-MB453 cell line lysates (35ug/lane) using Interleuki...