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anti-Human IL12B antibody for Immunohistochemistry (Frozen Sections)

Recommended IL12B Antibody (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) Antibodies
  • IL-12B
  • IL12B
  • p40
  • Il-12b
  • Il12p40
  • Il-12p40
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL12p40
  • IL-12
  • IL-12.p40
  • IL-12p40
  • interleukin 12 p40 subunit
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12b
  • interleukin 12B
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • IL-12
  • IL12A
  • Il12b
  • IL12B
Reactivity
Human
150
62
27
23
22
6
2
2
1
1
Host
Mouse
133
58
38
11
6
1
1
1
Clonality
Monoclonal
Conjugate
This IL12B antibody is un-conjugated
22
17
15
8
6
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
159
155
61
23
21
15
10
6
5
4
4
3
2
1
1
1
1
Supplier
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Catalog No. ABIN118938
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN255722 IHC (fro) IHC ELISA Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN2474931 IHC (fro) ELISA Mouse IgG1 Log in to see I-1A4 3
1 ABIN4325523 ELISA IHC IHC (fro) Alexa Fluor 488 Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325525 ELISA IHC IHC (fro) Alexa Fluor 700 Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325527 ELISA IHC IHC (fro) Biotin Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325528 ELISA IHC IHC (fro) DyLight 650 Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325529 ELISA IHC IHC (fro) FITC Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325531 ELISA IHC IHC (fro) DyLight 488 Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325532 ELISA IHC IHC (fro) DyLight 755 Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325537 ELISA IHC IHC (fro) DyLight 405 Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325533 ELISA IHC (fro) PerCP Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325536 ELISA IHC IHC (fro) DyLight 350 Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325522 ELISA IHC IHC (fro) Alexa Fluor 405 Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325524 ELISA IHC IHC (fro) Alexa Fluor 647 Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325535 ELISA IHC IHC (fro) DyLight 550 Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325530 ELISA IHC IHC (fro) DyLight 680 Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4226150 ELISA IHC IHC (fro) HRP Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325534 ELISA IHC (fro) PE Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325526 ELISA IHC (fro) Allophycocyanin Mouse IgG1 Subunit p40 Log in to see I-1A4
1 ABIN4325538 ELISA IHC (fro) DyLight 405LS Mouse IgG1 Subunit p40 Log in to see I-1A4

Top referenced anti-IL12B antibody for Immunohistochemistry (Frozen Sections)

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Application / Reactivity Human
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Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 22 Antibodies
Functional Studies (Func) 3 Antibodies
Immunoassay (IA) 2 Antibodies
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Western Blotting (WB) 94 Antibodies

General

Antigen Interleukin 12b (IL12B) Antibodies
Reactivity Human
(150), (62), (27), (23), (22), (6), (2), (2), (1), (1)
Host Mouse
(133), (58), (38), (11), (6), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL12B antibody is un-conjugated
(22), (17), (15), (8), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
(159), (155), (61), (23), (21), (15), (10), (6), (5), (4), (4), (3), (2), (1), (1), (1), (1)
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Product Details anti-IL12B Antibody

Target Details IL12B Application Details Handling Images
Specificity This antibody recognises the p40 subunit of interleukin-12, and binds to both the free subunit and the p35/p40 heterodimer. The antibody reacts with both stimulated and unstimulated monocytes in immunohistology.
Purification Purified
Immunogen Recombinant IL-12. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2-O myeloma cell line.
Clone 1-1A4
Isotype IgG1

Target Details IL12B

Product Details anti-IL12B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-12 beta / IL12B (IL12B Antibody Abstract)
Background Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2
Gene ID 3593
NCBI Accession NP_002178
UniProt P29460
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL12B Antibody Target Details IL12B Handling Images back to top
Application Notes Immunohistochemistry on frozen sections. ELISA: 1/1000 as detecting antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL12B Antibody Target Details IL12B Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.