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anti-Human IL12B antibody for Western Blotting

Recommended IL12B Antibody (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) Antibodies
  • CLMF
  • CLMF2
  • IL-12
  • IL-12.p40
  • IL-12B
  • Il-12b
  • IL-12p40
  • Il-12p40
  • Il12
  • IL12B
  • IL12p40
  • Il12p40
  • NKSF
  • NKSF2
  • p40
Epitope
AA 271-298, C-Term
29
19
14
12
12
11
7
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
149
59
27
23
20
6
2
2
1
1
Host
Rabbit
128
61
36
11
6
1
1
1
Clonality
Polyclonal
Conjugate
This IL12B antibody is un-conjugated
22
17
15
8
6
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
161
158
41
28
22
16
7
5
4
4
4
3
2
2
1
1
1
Supplier
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Catalog No. ABIN952920
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN2176964 WB Rabbit IgG AA 220-255 Log in to see Polyclonal 1
0.13341247 ABIN654420 FACS IHC (p) WB Rabbit Ig AA 264-290, C-Term Log in to see Polyclonal
0.13341247 ABIN3043199 WB Rabbit IgG AA 23-40, N-Term Log in to see Polyclonal
0.13341247 ABIN498502 WB Rabbit Log in to see Polyclonal
0.13341247 ABIN2706374 WB Rabbit Center Log in to see Polyclonal
0.13341247 ABIN2465844 IHC ELISA WB Goat Internal Region Log in to see Polyclonal
0.13341247 ABIN948031 ELISA WB Mouse IgG2a kappa AA 229-328, partial Log in to see 2H6
0.13341247 ABIN561472 ELISA WB Mouse AA 229-328, partial Log in to see Polyclonal
0.13341247 ABIN361134 ELISA WB Mouse IgG1, kappa chain Log in to see AT1D6
0.13341247 ABIN2487865 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.13341247 ABIN2489516 IHC WB Goat Log in to see Polyclonal
0.0011728023 ABIN374765 EIA IHC (p) WB Goat Internal Region Log in to see Polyclonal 1
0.0011728023 ABIN190760 IHC ELISA WB Goat Internal Region Log in to see Polyclonal 1
0.0011728023 ABIN377245 ELISA WB Mouse IgG1 AA 23-328 Log in to see AT1D6
0.0011728023 ABIN1102450 FACS IHC (p) Neut WB Mouse IgG1, kappa Log in to see C8-6
0.0011728023 ABIN296294 ELISA WB Goat Internal Region Log in to see Polyclonal
0.0011728023 ABIN2623862 WB Rabbit IgG AA 23-40 Log in to see Polyclonal
0.0011728023 ABIN603339 ELISA WB Human IgG1, kappa AA 23-328 Log in to see
0.0011728023 ABIN449748 SDS ELISA WB Mouse IgG1 kappa AA 23-328 Log in to see 1D6
0.0011728023 ABIN1724135 FACS IHC (p) WB Mouse IgG1 kappa Log in to see C8-6

Top referenced anti-IL12B antibody for Western Blotting

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Flow Cytometry (FACS) 22 Antibodies
Functional Studies (Func) 3 Antibodies
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General

Antigen Interleukin 12b (IL12B) Antibodies
Epitope AA 271-298, C-Term
(29), (19), (14), (12), (12), (11), (7), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(149), (59), (27), (23), (20), (6), (2), (2), (1), (1)
Host Rabbit
(128), (61), (36), (11), (6), (1), (1), (1)
Clonality
Polyclonal
Conjugate This IL12B antibody is un-conjugated
(22), (17), (15), (8), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(161), (158), (41), (28), (22), (16), (7), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL12B Antibody

Target Details IL12B Application Details Handling References for anti-IL12B Antibody (ABIN952920) Images
Specificity This antibody detects human Interleukin-12 beta/IL12Bat C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Purified through a protein A column; followed by peptide affinity purification.
Immunogen Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 271-298aa) of human Interleukin-12 beta/IL12B.
Isotype Ig

Target Details IL12B

Product Details anti-IL12B Antibody Application Details Handling References for anti-IL12B Antibody (ABIN952920) Images back to top
Antigen
Alternative Name Interleukin-12 beta / IL12B (IL12B Antibody Abstract)
Background IL12B is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2
Gene ID 3593
NCBI Accession NP_002178
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL12B Antibody Target Details IL12B Handling References for anti-IL12B Antibody (ABIN952920) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL12B Antibody Target Details IL12B Application Details References for anti-IL12B Antibody (ABIN952920) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL12B Antibody (ABIN952920)

Product Details anti-IL12B Antibody Target Details IL12B Application Details Handling Images back to top
Background publications

Sobti, Salih, Nega, Seyed, Rupinder, Vijesh, Ajay: "Insights into the role of IL-12B and IFN-gamma cytokine gene polymorphisms in HIV-1/AIDS infection." in: Folia biologica, Vol. 56, Issue 3, pp. 110-5, 2010

Torres de Heens, Loos, van der Velden: "Monozygotic twins are discordant for chronic periodontitis: white blood cell counts and cytokine production after ex vivo stimulation." in: Journal of clinical periodontology, Vol. 37, Issue 2, pp. 129-36, 2010

Images

Product Details anti-IL12B Antibody Target Details IL12B Application Details Handling References for anti-IL12B Antibody (ABIN952920) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL12B antibody (Interleukin 12b) (AA 271-298) (ABIN952920) Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-IL12B antibody (Interleukin 12b) (AA 271-298) (ABIN952920) Immunohistochemistry analysis in human tonsil tissue (formalin-fixed, paraffin-embedd...
Western Blotting (WB) image for anti-IL12B antibody (Interleukin 12b) (AA 271-298) (ABIN952920) Western blot analysis in CEM,MDA-MB453 cell line lysates (35ug/lane) using Interleuki...