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Cat (Feline) IL12B ELISA Kit

Recommended IL12B Product (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) ELISA Kits
  • CLMF
  • CLMF2
  • IL-12
  • IL-12.p40
  • IL-12B
  • Il-12b
  • IL-12p40
  • Il-12p40
  • Il12
  • IL12B
  • IL12p40
  • Il12p40
  • NKSF
  • NKSF2
  • p40
Reactivity
Cat (Feline)
Alternatives
Kits with alternative reactivity to:
31
12
9
7
5
5
4
4
3
3
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
0.16-40 ng/mL
Minimum Detection Limit
0.16 ng/mL
Application
ELISA
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Catalog No. ABIN2703169
$ 654.50
Plus shipping costs $45.00

Similar IL12B ELISA Kits

Application / Reactivity Cat (Feline)
ELISA 1 ELISA Kits

General

Antigen Interleukin 12b (IL12B) ELISA Kits
Reactivity Cat (Feline)
Kits with alternative reactivity to:
(31), (12), (9), (7), (5), (5), (4), (4), (3), (3), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 0.16-40 ng/mL
Minimum Detection Limit 0.16 ng/mL
Application ELISA
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Product Details IL12B ELISA Kit

Target details Application Details Handling Images
Sample Type Cell Culture Supernatant, Plasma, Serum
Detection Method Colorimetric
Specificity Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: feline Fas, IFN-gamma, IL-1 beta, IL-2, IL-4, IL-5, IL-8, IL-10, RANTES
Sensitivity 0.16 ng/ml

Target details

Product Details IL12B ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name IL-12p40 (IL12B ELISA Kit Abstract)
Background Gene Names: IL12B
Protein names: Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40)
Gene ID 768273
UniProt O02744
Pathways JAK-STAT Signaling

Application Details

Product Details IL12B ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution: 2 fold
Plate Strips
Protocol Feline IL-12p40 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of feline IL-12p40 in serum (feline IL-12p40 concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for feline IL-12p40 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12p40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-feline IL-12p40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12p40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Restrictions For Research Use only

Handling

Product Details IL12B ELISA Kit Target details Application Details Images back to top
Storage 4 °C