Cat (Feline) IL12B ELISA Kit

Recommended IL12B Product (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) ELISA Kits
  • IL-12B
  • IL12B
  • p40
  • Il-12b
  • Il12p40
  • Il-12p40
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL12p40
  • IL-12
  • IL-12.p40
  • IL-12p40
  • interleukin 12 p40 subunit
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12b
  • interleukin 12B
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • IL-12
  • IL12A
  • Il12b
  • IL12B
Reactivity
Cat (Feline)
Alternatives
Kits with alternative reactivity to:
38
19
12
10
10
8
8
6
6
5
3
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
0.16-40 ng/mL
Minimum Detection Limit
0.16 ng/mL
Application
ELISA
Options
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Catalog No. ABIN2703169
$ 666.60
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN5596592 Sandwich ELISA Log in to see

General

Antigen Interleukin 12b (IL12B) ELISA Kits
Reactivity Cat (Feline)
Kits with alternative reactivity to:
(38), (19), (12), (10), (10), (8), (8), (6), (6), (5), (3), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 0.16-40 ng/mL
Minimum Detection Limit 0.16 ng/mL
Application ELISA
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Product Details IL12B ELISA Kit

Target details Application Details Handling Images
Purpose Feline IL-12 p40 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Cell Culture Supernatant, Plasma, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: feline Fas, IFN-gamma, IL-1 beta, IL-2, IL-4, IL-5, IL-8, IL-10, RANTES
Sensitivity 0.16 ng/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IL12B ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name IL-12p40 (IL12B ELISA Kit Abstract)
Background Gene Names: IL12B
Protein names: Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40)
Gene ID 768273
UniProt O02744
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details IL12B ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details IL12B ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

Images

Product Details IL12B ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Interleukin 12b (IL12B) ELISA Kit (ABIN2703169) Interleukin 12b (IL12B) ELISA Kit