Human IL12B ELISA Kit

Recommended IL12B Product (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) ELISA Kits
  • IL-12B
  • IL12B
  • p40
  • Il-12b
  • Il12p40
  • Il-12p40
  • Il12
  • CLMF
  • CLMF2
  • NKSF
  • NKSF2
  • IL12p40
  • IL-12
  • IL-12.p40
  • IL-12p40
  • interleukin 12 p40 subunit
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin 12b
  • interleukin 12B
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
  • IL-12
  • IL12A
  • Il12b
  • IL12B
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
35
17
11
9
9
7
7
5
5
4
3
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
10-3000 pg/mL
Minimum Detection Limit
10 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625009
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.70292 ABIN411311 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate) 31.2-2000 pg/mL 31.2 pg/mL Log in to see 12
9.70292 ABIN365211 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
9.70292 ABIN414874 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
9.70292 ABIN1115613 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
9.70292 ABIN2708259 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
9.70292 ABIN2708260 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
9.70292 ABIN2064512 Cell Culture Supernatant, Serum, Plasma Log in to see
1 ABIN2851250 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN4993111 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 12.3-3000 pg/mL 12.3 pg/mL Log in to see
1 ABIN1446078 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples Log in to see 3
1 ABIN5593114 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN455525 Sandwich ELISA Tissue Homogenate, Cell Culture Supernatant, Serum, Plasma, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN2684574 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 31.2-2000 pg/mL 31.2 pg/mL Log in to see 3
1 ABIN1503290 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate 0.5-10 ng/mL 0.5 ng/mL Log in to see
1 ABIN5522340 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN2748834 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN2684575 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Plasma, Serum 62.5-2000 pg/mL 62.5 pg/mL Log in to see
1 ABIN4986928 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA) 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN1112721 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN2648767 Serum, Plasma, Cell Culture Cells Log in to see

Top referenced IL12B ELISA Kit for ELISA

  • Human IL12B ELISA Kit for Sandwich ELISA - ABIN411311 (12 Publications): Becher, Durell, Noelle: Experimental autoimmune encephalitis and inflammation in the absence of interleukin-12. in The Journal of clinical investigation 2002 (PubMed)

  • Human IL12B ELISA Kit for Sandwich ELISA - ABIN625009 (3 Publications): Chowdhury, Ahmed, Choudhuri, Sen, Hazra, Pal, Bhattacharya, Bahar: Alteration of serum inflammatory cytokines in active pulmonary tuberculosis following anti-tuberculosis drug therapy. in Molecular immunology 2014 (PubMed)

  • Human IL12B ELISA Kit for Sandwich ELISA - ABIN1446078 (3 Publications): Vilisaar, Kawabe, Braitch, Aram, Furtun, Fahey, Chopra, Tanasescu, Tighe, Gran, Pothoulakis, Constantinescu: Reciprocal Regulation of Substance P and IL-12/IL-23 and the Associated Cytokines, IFNγ/IL-17: A Perspective on the Relevance of This Interaction to Multiple Sclerosis. in Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2015 (PubMed)

  • Human IL12B ELISA Kit for Sandwich ELISA - ABIN2684574 (3 Publications): Cua, Sherlock, Chen, Murphy, Joyce, Seymour, Lucian, To, Kwan, Churakova, Zurawski, Wiekowski, Lira, Gorman, Kastelein, Sedgwick: Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain. in Nature 2003 (PubMed)

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General

Antigen Interleukin 12b (IL12B) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(35), (17), (11), (9), (9), (7), (7), (5), (5), (4), (3), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 10-3000 pg/mL
Minimum Detection Limit 10 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details IL12B ELISA Kit

Target details Application Details Handling ProductDetails: References for IL12B Kit (ABIN625009) Images
Purpose Human IL-12 p40 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity < 10 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IL12B ELISA Kit Application Details Handling ProductDetails: References for IL12B Kit (ABIN625009) Images back to top
Antigen
Alternative Name IL-12 p40 (IL12B ELISA Kit Abstract)
Background The Human IL-12(P40) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-12(P40) in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human IL-12(P40) coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12(P40) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-12(P40) antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12(P40) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
Gene ID 3593
UniProt P29460
Pathways JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details IL12B ELISA Kit Target details Handling ProductDetails: References for IL12B Kit (ABIN625009) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent C (Item L) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants and urine. Suggested dilution for normal serum/plasma: 2 fold*. *Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water before use.
4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent C (for serum/plasma samples) or 1x Assay Diluent B (for cell culture supernates/urine) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 30 µL IL-12(P40) standard (50 ng/mL) from the vial of Item C, into a tube with 470 µL Assay Diluent C or 1x Assay Diluent B to prepare a 3,000 pg/mL standard solution. Pipette 300 µL Assay Diluent C or 1x Assay Diluent B into each tube. Use the 3,000 pg/mL standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent C or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 30 µL standard + 470 µL 200 µL 200 µL 200 µL 200 µL 200 µL 200myl 3,000 1,200 480 192 76.8 30.72 12.29 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 400-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 30 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare a final 400 fold diluted HRP- Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Human IL-12(P40) concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent C Human IL-12(P40) concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B
Sensitivity: The minimum detectable dose of IL-12(P40) is typically less than 10 pg/mL.
Recovery: Recovery was determined by spiking various levels of human IL-12(P40) into human serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 99.73 91-108 Plasma 91.11 85-98 Cell culture media 127.6 120-134
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 107.6 104.2 105.9 Range ( %) 99-115 95-114 98-113 1:4 Average % of Expected 104.1 101.4 85.99 Range ( %) 96-111 96-108 77-94
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details IL12B ELISA Kit Target details Application Details ProductDetails: References for IL12B Kit (ABIN625009) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

ProductDetails: References for IL12B Kit (ABIN625009)

Product Details IL12B ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Bishayi, Bandyopadhyay, Majhi, Adhikary et al.: "Expression of CXCR1 (interleukin-8 receptor) in murine macrophages after staphylococcus aureus infection and its possible implication on intracellular survival correlating with cytokines and ..." in: Inflammation, Vol. 38, Issue 2, pp. 812-27, 2015

Chowdhury, Ahmed, Choudhuri, Sen, Hazra, Pal, Bhattacharya, Bahar: "Alteration of serum inflammatory cytokines in active pulmonary tuberculosis following anti-tuberculosis drug therapy." in: Molecular immunology, Vol. 62, Issue 1, pp. 159-68, 2014

Abu Alshamat, Al-Okla, Soukkarieh, Kweider: "Human chorionic gonadotrophin (hCG) enhances immunity against L. tropica by stimulating human macrophage functions." in: Parasite immunology, Vol. 34, Issue 10, pp. 449-54, 2012

Images

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Supplier Images
ELISA image for IL12B ELISA Kit (Interleukin 12b) (ABIN625009) Interleukin 12b (IL12B) ELISA Kit