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Human IL12B ELISA Kit

Recommended IL12B Product (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) ELISA Kits
  • CLMF
  • CLMF2
  • IL-12
  • IL-12.p40
  • IL-12B
  • Il-12b
  • IL-12p40
  • Il-12p40
  • Il12
  • IL12B
  • IL12p40
  • Il12p40
  • NKSF
  • NKSF2
  • p40
Epitope
AA 23-328
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
30
12
9
7
5
5
4
4
3
3
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN411311
$ 250.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN414874 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32693866 ABIN1115613 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32693866 ABIN2708259 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.32693866 ABIN2708260 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.32693866 ABIN2064512 Cell Culture Supernatant, Serum, Plasma Log in to see
0.32693866 ABIN2762258 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN365211 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012117105 ABIN1446078 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples Log in to see 3
0.0012117105 ABIN625009 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 2
0.0012117105 ABIN455525 Sandwich ELISA Tissue Homogenate, Cell Culture Supernatant, Serum, Plasma, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2684574 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 31.2-2000 pg/mL 31.2 pg/mL Log in to see 3
0.0012117105 ABIN1112721 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2748834 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN2684575 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Plasma, Serum 62.5-2000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN1503290 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012117105 ABIN2792490 Cell Culture Supernatant, Serum, Plasma Log in to see
0.0012117105 ABIN2042519 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2116075 3.12-200 pg/mL 3.12 pg/mL Log in to see
0.0012117105 ABIN1846943 Tissue Homogenate, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2648767 Serum, Plasma, Cell Culture Cells Log in to see

Top referenced IL12B ELISA Kit for ELISA

  • Human IL12B ELISA Kit for Sandwich ELISA - ABIN411311 (8 Publications): Becher, Durell, Noelle: Experimental autoimmune encephalitis and inflammation in the absence of interleukin-12. in The Journal of clinical investigation 2002 (PubMed)

  • Human IL12B ELISA Kit for Sandwich ELISA - ABIN1446078 (3 Publications): Vilisaar, Kawabe, Braitch, Aram, Furtun, Fahey, Chopra, Tanasescu, Tighe, Gran, Pothoulakis, Constantinescu: Reciprocal Regulation of Substance P and IL-12/IL-23 and the Associated Cytokines, IFNγ/IL-17: A Perspective on the Relevance of This Interaction to Multiple Sclerosis. in Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2015 (PubMed)

  • Human IL12B ELISA Kit for Sandwich ELISA - ABIN625009 (2 Publications): Chowdhury, Ahmed, Choudhuri, Sen, Hazra, Pal, Bhattacharya, Bahar: Alteration of serum inflammatory cytokines in active pulmonary tuberculosis following anti-tuberculosis drug therapy. in Molecular immunology 2014 (PubMed)

  • Human IL12B ELISA Kit for Sandwich ELISA - ABIN2684574 (3 Publications): Cua, Sherlock, Chen, Murphy, Joyce, Seymour, Lucian, To, Kwan, Churakova, Zurawski, Wiekowski, Lira, Gorman, Kastelein, Sedgwick: Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain. in Nature 2003 (PubMed)

Similar IL12B ELISA Kits

Application / Reactivity Human
ELISA 31 ELISA Kits

General

Antigen Interleukin 12b (IL12B) ELISA Kits
Epitope AA 23-328
Reactivity Human
Kits with alternative reactivity to:
(30), (12), (9), (7), (5), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 8 references available
Supplier Log in to see

Product Details IL12B ELISA Kit

Target details Application Details Handling References for IL12B Kit (ABIN411311) Images
Purpose For quantitative detection of human IL-12(P40) in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant human IL-12(P40)
Cross-Reactivity (Details) There is cross-reactivates with IL-12(p70), IL-23(p19/p40)< 5 % .
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- human IL-12(P40) antibod - 1
  • Lyophilized recombinant human IL-12(P40) standar - 10ng/tubex2
  • Biotinylated anti- human IL-12(P40) antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: I23-S328

Target details

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Antigen
Alternative Name IL12B (IL12B ELISA Kit Abstract)
Background Interleukin-12(IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits. The larger 40- kDa subunit(p40) is a member of the cytokine receptor family, and the smaller 35- kDa subunit(p35) is related to IL6 and GCSF. Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer. Interleukin(IL)-12 has been cloned on the basis of its ability to activate natural killer(NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon(IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection, for the development of a Th1 response, critical for effective host defense against intracellular pathogens, and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype. The subunits map to different chromosomes: p40(IL12B) to 5q31-q33 and p35(IL12A) to 3p12-3q13.2.2 The standard product used in this kit is recombinant human IL-12(p40), which is a 40KDa glycoprotein consisting of 306 amino acids.
Gene ID 3593
UniProt P29460
Research Area Hormones, Cytokines
Pathways JAK-STAT Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IL-12(P40) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-12(P40) has been precoated onto 96-well plates. Standards(sf21, I23-S328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(P40) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-12(P40) amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-12(P40) standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-12(P40) standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 276, Standard deviation: 16.56, CV(%): 6
  • Sample 2: n=16, Mean(pg/ml): 772, Standard deviation: 41.7, CV(%): 5.4
  • Sample 3: n=16, Mean(pg/ml): 1230, Standard deviation: 59.04, CV(%): 4.8,
  • Sample 1: n=24, Mean(pg/ml): 316, Standard deviation: 23.07, CV(%): 7.3
  • Sample 2: n=24, Mean(pg/ml): 869, Standard deviation: 56.5, CV(%): 6.5
  • Sample 3: n=24, Mean(pg/ml): 1475, Standard deviation: 78.2, CV(%): 5.3
Restrictions For Research Use only

Handling

Product Details IL12B ELISA Kit Target details Application Details References for IL12B Kit (ABIN411311) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL12B Kit (ABIN411311)

Product Details IL12B ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Li, Li, Liu, Zhuang, Yang, Zhang: "Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin-12 DNA induces E7-specific antitumor effects in C57BL/6 mice." in: Oncology letters, Vol. 7, Issue 2, pp. 576-582, 2014

Ma, He, Han, Yang, Ji: "A clinical study of HBsAg-activated dendritic cells and cytokine-induced killer cells during the treatment for chronic hepatitis B." in: Scandinavian journal of immunology, Vol. 78, Issue 4, pp. 387-93, 2013

Hara, Mimura, Abe, Shioi, Seiki, Sakamoto: "Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32542-51, 2011

Li, Yu, Ye, Wang, Yang: "Lactobacillus S-layer protein inhibition of Salmonella-induced reorganization of the cytoskeleton and activation of MAPK signalling pathways in Caco-2 cells." in: Microbiology (Reading, England), Vol. 157, Issue Pt 9, pp. 2639-46, 2011

Background publications

Cua, Sherlock, Chen, Murphy, Joyce, Seymour, Lucian, To, Kwan, Churakova, Zurawski, Wiekowski, Lira, Gorman, Kastelein, Sedgwick: "Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain." in: Nature, Vol. 421, Issue 6924, pp. 744-8, 2003

Becher, Durell, Noelle: "Experimental autoimmune encephalitis and inflammation in the absence of interleukin-12." in: The Journal of clinical investigation, Vol. 110, Issue 4, pp. 493-7, 2002

Wolf, Sieburth, Sypek: "Interleukin 12: a key modulator of immune function." in: Stem cells (Dayton, Ohio), Vol. 12, Issue 2, pp. 154-68, 1994

Sieburth, Jabs, Warrington, Li, Lasota, LaForgia, Kelleher, Huebner, Wasmuth, Wolf: "Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5." in: Genomics, Vol. 14, Issue 1, pp. 59-62, 1992

Images

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Supplier Images
ELISA image for IL12B ELISA Kit (Interleukin 12b) (ABIN411311) Human IL-12(p40) PicoKine ELISA Kit standard curve