anti-Human IL15 antibody for Enzyme Immunoassay

Recommended IL15 Antibody (supplied by: Log in to see )

Antigen
Interleukin 15 (IL15) Antibodies
  • IL15
  • AI503618
  • IL-15
  • interleukin 15
  • IL15
  • Il15
Reactivity
Human
239
67
27
10
8
6
5
2
2
1
1
1
Host
Rabbit
146
136
18
17
5
5
Clonality
Polyclonal
Conjugate
This IL15 antibody is conjugated to Biotin
36
17
11
10
8
6
6
6
6
5
5
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
209
183
66
48
32
29
27
17
13
10
10
7
5
4
4
3
3
3
1
1
1
1
1
1
1
1
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Catalog No. ABIN116109
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN116110 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN115774 EIA WB Mouse IgG1 Log in to see
1 ABIN263855 EIA IHC (fro) IP WB Mouse IgG1 Log in to see BDI150
1 ABIN116111 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN116112 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN5565741 EIA RIA WB Rabbit AA 49-162 Log in to see Polyclonal

General

Antigen Interleukin 15 (IL15) Antibodies
Reactivity Human
(239), (67), (27), (10), (8), (6), (5), (2), (2), (1), (1), (1)
Host Rabbit
(146), (136), (18), (17), (5), (5)
Clonality
Polyclonal
Conjugate This IL15 antibody is conjugated to Biotin
(36), (17), (11), (10), (8), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(209), (183), (66), (48), (32), (29), (27), (17), (13), (10), (10), (7), (5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL15 Antibody

Target Details IL15 Application Details Handling Images
Specificity This antibody reacts with Human Interleukin-15. Other species not tested.
Purification Affinity chromatography.
Immunogen Highly pure (>98%) recombinant hIL-15 (human IL-15).

Target Details IL15

Product Details anti-IL15 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-15 / IL15 (IL15 Antibody Abstract)
Background IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97 % and 73 % amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle. IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.Synonyms: IL-15
Gene ID 3600
NCBI Accession NP_000576
UniProt P40933
Pathways JAK-STAT Signaling, Glycosaminoglycan Metabolic Process

Application Details

Product Details anti-IL15 Antibody Target Details IL15 Handling Images back to top
Application Notes ELISA: To detect hIL-15 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-15. Western Blot: To detect hIL-15 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents
Restrictions For Research Use only

Handling

Product Details anti-IL15 Antibody Target Details IL15 Application Details Images back to top
Reconstitution Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.