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anti-Mouse (Murine) IL15 antibody for Enzyme Immunoassay

Recommended IL15 Antibody (supplied by: Log in to see )

Antigen
Interleukin 15 (IL15) Antibodies
  • AI503618
  • IL-15
  • IL15
Reactivity
Mouse (Murine)
165
43
22
7
6
6
3
1
1
Host
Rabbit
130
71
10
7
5
4
Clonality
Polyclonal
Conjugate
This IL15 antibody is conjugated to Biotin
30
15
9
5
5
5
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
157
155
35
26
22
16
11
9
8
7
6
6
5
5
3
1
1
1
1
1
Supplier
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Catalog No. ABIN181593
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN181596 EIA Func WB Rabbit Log in to see Polyclonal

Top referenced anti-IL15 antibody for Enzyme Immunoassay

Similar anti-IL15 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 23 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Functional Studies (Func) 3 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Neutralization (Neut) 10 Antibodies
Western Blotting (WB) 34 Antibodies

General

Antigen Interleukin 15 (IL15) Antibodies
Reactivity Mouse (Murine)
(165), (43), (22), (7), (6), (6), (3), (1), (1)
Host Rabbit
(130), (71), (10), (7), (5), (4)
Clonality
Polyclonal
Conjugate This IL15 antibody is conjugated to Biotin
(30), (15), (9), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(157), (155), (35), (26), (22), (16), (11), (9), (8), (7), (6), (6), (5), (5), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL15 Antibody

Target Details IL15 Application Details Handling References for anti-IL15 Antibody (ABIN181593) Images
Specificity This antibody detects Interleukin-15.
Purification Affinity Chromatography
Immunogen Highly pure (> 98%) recombinant Murine IL-15

Target Details IL15

Product Details anti-IL15 Antibody Application Details Handling References for anti-IL15 Antibody (ABIN181593) Images back to top
Antigen
Alternative Name Interleukin-15 / IL15 (IL15 Antibody Abstract)
Background IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97 % and 73 % amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle. IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.Synonyms: IL-15
Gene ID 16168
NCBI Accession NP_032383
UniProt P48346
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL15 Antibody Target Details IL15 Handling References for anti-IL15 Antibody (ABIN181593) Images back to top
Application Notes Direct ELISA: To detect Murine IL-15 by Direct ELISA (using 100 μL/well antibody solution) aconcentration of approx. 1.0 μg/mL of this antibody is required. This Biotin conjugated antibody allows the detection of at least 0.2-0.4 ng/well ofrecombinant Murine IL-15. Sandwich ELISA: To detect Murine IL-15 by Sandwich ELISA (using 100 μL/well antibody
Restrictions For Research Use only

Handling

Product Details anti-IL15 Antibody Target Details IL15 Application Details References for anti-IL15 Antibody (ABIN181593) Images back to top
Reconstitution Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
Expiry Date 6 months

References for anti-IL15 Antibody (ABIN181593)

Product Details anti-IL15 Antibody Target Details IL15 Application Details Handling Images back to top
Background publications

Miyazaki, Ishikawa, Shimizu, Ubagai, Edelstein, Yamaguchi: "Gr-1high polymorphonuclear leukocytes and NK cells act via IL-15 to clear intracellular Haemophilus influenzae in experimental murine peritonitis and pneumonia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 8, pp. 5407-14, 2007