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Human IL15 ELISA Kit

Recommended IL15 Product (supplied by: Log in to see )

Antigen
Interleukin 15 (IL15) ELISA Kits
  • AI503618
  • IL-15
  • IL15
Epitope
AA 49-162
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
38
15
10
8
6
6
6
5
5
3
2
2
1
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
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Catalog No. ABIN411313
$ 300.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN2472074 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.30628014 ABIN414877 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 1
0.30628014 ABIN924854 Sandwich ELISA Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.30628014 ABIN2708264 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.30628014 ABIN2064464 Cell Culture Supernatant, Serum, Plasma Log in to see
0.30628014 ABIN2762263 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.30628014 ABIN2114270 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
0.30628014 ABIN1685886 Sandwich ELISA Serum, Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Urine 3 pg/mL Log in to see
0.0012155735 ABIN365226 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant 3.12-200 pg/mL 3.12 pg/mL Log in to see
0.0012155735 ABIN3032941 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 3.3-800 pg/mL 3.3 pg/mL Log in to see
0.0012155735 ABIN626498 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012155735 ABIN1115618 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012155735 ABIN1446083 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples 18.3pg/mL Log in to see
0.0012155735 ABIN625012 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 4
0.0012155735 ABIN2684586 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see 2
0.0012155735 ABIN1028965 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012155735 ABIN1112726 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN2748841 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012155735 ABIN2042525 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN2114271 Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see

Top referenced IL15 ELISA Kit for ELISA

Similar IL15 ELISA Kits

Application / Reactivity Human
ELISA 38 ELISA Kits

General

Antigen Interleukin 15 (IL15) ELISA Kits
Epitope AA 49-162
Reactivity Human
Kits with alternative reactivity to:
(38), (15), (10), (8), (6), (6), (6), (5), (5), (3), (2), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details IL15 ELISA Kit

Target details Application Details Handling References for IL15 Kit (ABIN411313) Images
Purpose For quantitative detection of human IL-15 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human IL-15
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <3pg/mL
Components
  • 96-well plate precoated with anti- human IL-15 antibod - 1
  • Lyophilized recombinant human IL-15 standar - 10ng/tubex2
  • Biotinylated anti- human IL-15 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: N49-S162

Target details

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Antigen
Alternative Name IL15 (IL15 ELISA Kit Abstract)
Background Interleukin(IL)-15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2(IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor. IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection. The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.1 The standard product used in this kit is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9KDa.
Gene ID 3600
UniProt P40933
Research Area Immunology, Innate Immunity, Cytokines
Pathways JAK-STAT Signaling

Application Details

Product Details IL15 ELISA Kit Target details Handling References for IL15 Kit (ABIN411313) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IL-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-15 has been precoated onto 96-well plates. Standards(E.coli, N49-S162) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-15 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human IL-15 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-15 standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 125, Standard deviation: 5.25, CV(%): 4.2
  • Sample 2: n=16, Mean(pg/ml): 372, Standard deviation: 18.23, CV(%): 4.9
  • Sample 3: n=16, Mean(pg/ml): 603, Standard deviation: 31.36, CV(%): 5.2,
  • Sample 1: n=24, Mean(pg/ml): 170, Standard deviation: 9.01, CV(%): 5.3
  • Sample 2: n=24, Mean(pg/ml): 418, Standard deviation: 27.17, CV(%): 6.5
  • Sample 3: n=24, Mean(pg/ml): 679, Standard deviation: 48.21, CV(%): 7.1
Restrictions For Research Use only

Handling

Product Details IL15 ELISA Kit Target details Application Details References for IL15 Kit (ABIN411313) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL15 Kit (ABIN411313)

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Background publications

Conti, Frappier, Dharancy, Chereau, Houssin, Weill, Calmus: "Interleukin-15 production during liver allograft rejection in humans." in: Transplantation, Vol. 76, Issue 1, pp. 210-6, 2003

Anderson, Johnson, Glaccum, Copeland, Gilbert, Jenkins, Valentine, Kirstein, Shapiro, Morris: "Chromosomal assignment and genomic structure of Il15." in: Genomics, Vol. 25, Issue 3, pp. 701-6, 1995

Images

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Supplier Images
ELISA image for IL15 ELISA Kit (Interleukin 15) (ABIN411313) Human IL-15 PicoKine ELISA Kit standard curve