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anti-Human IL15RA antibody for Flow Cytometry

Recommended IL15RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 15 Receptor, alpha (IL15RA) Antibodies
  • AA690181
  • CD215
  • IL-15RA
  • IL15RA
Reactivity
Human
70
25
23
Host
Mouse
57
14
4
1
Clonality
Monoclonal
Conjugate
This IL15RA antibody is conjugated to Biotin
5
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Application
Complement Mediated Cell Depletion (CMCD), Depletion (Dep), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
53
40
11
11
6
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN2660994
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN2663180 FACS PE Mouse IgG2b kappa Log in to see JM7A4 10
0.0010482287 ABIN473563 FACS FITC Mouse IgG2b Log in to see JM7A4
0.0010482287 ABIN477202 FACS IP WB Mouse IgG2b Log in to see JM7A4
0.0010482287 ABIN2663181 FACS PE Mouse IgG2b kappa Log in to see JM7A4 10
0.0010482287 ABIN281660 FACS IP WB Mouse IgG2b Log in to see JM7A4
0.0010482287 ABIN2680318 FACS FITC Mouse IgG2b Log in to see JM7A4
0.0010482287 ABIN2144032 FACS Mouse IgG2b Log in to see JM7A4

Top referenced anti-IL15RA antibody for Flow Cytometry

Similar anti-IL15RA Antibodies

Application / Reactivity Human
Complement Mediated Cell Depletion (CMCD) 1 Antibodies
Depletion (Dep) 1 Antibodies
ELISA 38 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Gel Shift (GS) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 51 Antibodies

General

Antigen Interleukin 15 Receptor, alpha (IL15RA) Antibodies
Reactivity Human
(70), (25), (23)
Host Mouse
(57), (14), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL15RA antibody is conjugated to Biotin
(5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Complement Mediated Cell Depletion (CMCD), Depletion (Dep), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(53), (40), (11), (11), (6), (3), (2), (2), (2), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-IL15RA Antibody

Target Details IL15RA Application Details Handling References for anti-IL15RA Antibody (ABIN2660994) Images
Purification The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Clone JM7A4
Isotype IgG2b kappa

Target Details IL15RA

Product Details anti-IL15RA Antibody Application Details Handling References for anti-IL15RA Antibody (ABIN2660994) Images back to top
Antigen
Alternative Name CD215 (IL15RA Antibody Abstract)
Background IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology to IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains. Similar to IL-2Rα, IL-15Rα is able to form a heterotrimer with their β chain (CD122) and common γ chain (γc, CD132). But unlike IL-2Rα subunit, IL-15Rα itself binds to IL-15 with equivalent high affinity compared with the heterotrimeric complex. IL-15Rα is expressed as soluble or transmembrane forms by variety cells, such as activated monocytes, dendritic cells, CD8+ memory T cells, NK or NKT cells. IL-15Rα by itself forms stable complexes with IL-15 on cell surface and presents IL-15 in trans to neighboring cells. IL-15, like IL-2, is a pleiotropic four-helix bundle cytokine and plays a key role in promoting T and B cell survival and proliferation, preferentially in memory CD8+ T cell proliferation, and NK/NKT cell development.
Pathways JAK-STAT Signaling, TCR Signaling

Application Details

Product Details anti-IL15RA Antibody Target Details IL15RA Handling References for anti-IL15RA Antibody (ABIN2660994) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL15RA Antibody Target Details IL15RA Application Details References for anti-IL15RA Antibody (ABIN2660994) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-IL15RA Antibody (ABIN2660994)

Product Details anti-IL15RA Antibody Target Details IL15RA Application Details Handling Images back to top
Background publications

Kitaya, Yasuo: "Regulatory role of membrane-bound form interleukin-15 on human uterine microvascular endothelial cells in circulating CD16(-) natural killer cell extravasation into human endometrium." in: Biology of reproduction, Vol. 89, Issue 3, pp. 70, 2013

Veerman, Carlow, Shanina, Priatel, Horwitz, Ziltener: "PSGL-1 regulates the migration and proliferation of CD8(+) T cells under homeostatic conditions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 4, pp. 1638-46, 2012

Vujanovic, Szymkowski, Alber, Watkins, Vujanovic, Butterfield: "Virally infected and matured human dendritic cells activate natural killer cells via cooperative activity of plasma membrane-bound TNF and IL-15." in: Blood, Vol. 116, Issue 4, pp. 575-83, 2010

Sato, Patel, Waldmann, Tagaya: "The IL-15/IL-15Ralpha on cell surfaces enables sustained IL-15 activity and contributes to the long survival of CD8 memory T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 2, pp. 588-93, 2007

Lorenzen, Dingley, Jacques, Grötzinger: "The structure of the interleukin-15 alpha receptor and its implications for ligand binding." in: The Journal of biological chemistry, Vol. 281, Issue 10, pp. 6642-7, 2006

Giron-Michel, Giuliani, Fogli, Brouty-Boyé, Ferrini, Baychelier, Eid, Lebousse-Kerdilès, Durali, Biassoni, Charpentier, Vasquez, Chouaib, Caignard, Moretta, Azzarone: "Membrane-bound and soluble IL-15/IL-15Ralpha complexes display differential signaling and functions on human hematopoietic progenitors." in: Blood, Vol. 106, Issue 7, pp. 2302-10, 2005

Vámosi, Bodnár, Vereb, Jenei, Goldman, Langowski, Tóth, Mátyus, Szöllösi, Waldmann, Damjanovich: "IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 30, pp. 11082-7, 2004

Dubois, Mariner, Waldmann, Tagaya: "IL-15Ralpha recycles and presents IL-15 In trans to neighboring cells." in: Immunity, Vol. 17, Issue 5, pp. 537-47, 2002

Giri, Kumaki, Ahdieh, Friend, Loomis, Shanebeck, DuBose, Cosman, Park, Anderson: "Identification and cloning of a novel IL-15 binding protein that is structurally related to the alpha chain of the IL-2 receptor." in: The EMBO journal, Vol. 14, Issue 15, pp. 3654-63, 1995

Images

Product Details anti-IL15RA Antibody Target Details IL15RA Application Details Handling References for anti-IL15RA Antibody (ABIN2660994) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL15RA antibody (Interleukin 15 Receptor, alpha)  (Biotin) (ABIN2660994) anti-Interleukin 15 Receptor, alpha (IL15RA) antibody (Biotin)