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anti-Mouse (Murine) IL15RA antibody for Functional Studies

Recommended IL15RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 15 Receptor, alpha (IL15RA) Antibodies
  • IL15RA
  • CD215
  • AA690181
  • IL-15RA
  • interleukin 15 receptor, alpha
  • interleukin 15 receptor, alpha chain
  • IL15RA
  • Il15ra
Epitope
Extracellular Domain
11
11
9
4
3
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
72
28
26
1
1
1
1
1
1
1
Host
Goat
59
13
3
1
Clonality
Polyclonal
Conjugate
This IL15RA antibody is un-conjugated
4
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Neutralization (Neut), ELISA, Western Blotting (WB)
53
38
13
9
6
3
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN327397
$ 969.83
Plus shipping costs $45.00

Similar anti-IL15RA Antibodies

Application / Reactivity Mouse (Murine)
ELISA 7 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Functional Studies (Func) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Neutralization (Neut) 2 Antibodies
Western Blotting (WB) 12 Antibodies

General

Antigen Interleukin 15 Receptor, alpha (IL15RA) Antibodies
Epitope Extracellular Domain
(11), (11), (9), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(72), (28), (26), (1), (1), (1), (1), (1), (1), (1)
Host Goat
(59), (13), (3), (1)
Clonality
Polyclonal
Conjugate This IL15RA antibody is un-conjugated
(4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Neutralization (Neut), ELISA, Western Blotting (WB)
(53), (38), (13), (9), (6), (3), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-IL15RA Antibody

Target Details IL15RA Application Details Handling Images
Specificity Recognizes mouse IL-15Ra.
Purification Immunoaffinity purified
Immunogen NS0-derived rmIL-15Ra extracellular domain.

Type of Immunogen: Recombinant protein
Isotype IgG

Target Details IL15RA

Product Details anti-IL15RA Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL15RA (IL15RA Antibody Abstract)
Background Name/Gene ID: IL15RA
Family: Interleukin

Synonyms: IL15RA, CD215 antigen, IL-15RA, IL-15 receptor subunit alpha, IL-15R-alpha, Interleukin 15 receptor, alpha, CD215
Gene ID 3601
UniProt Q13261
Research Area Signaling, Apoptosis/Necrosis
Pathways JAK-STAT Signaling, TCR Signaling

Application Details

Product Details anti-IL15RA Antibody Target Details IL15RA Handling Images back to top
Application Notes Approved: ELISA, Flo, Func, Neut, WB

Usage: Neutralization of bioactivity, Direct ELISA, Western Blot, Flow Cytometry. Neutralization of Mouse IL-15 R Bioactivity: The exact concentration of antibody required to neutralize the mouse cell surface IL-15 R mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-15 R mediated IL-15 response on a responsive cell line, at a specific IL-15 concentration. As shown in Figures 1 and 2 on the next page, the ND50 for this lot of anti-mouse IL-15 R antibody was determined to be approximately 0.3-1.5 μg/mL in the presence of 0.8 ng/mL of human IL-15, using the CTLL-2 cell proliferation assay. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 0.1 ng/well. In this format, this antibody shows approximately 5 % cross-reactivity with rhIL-15 R and less than 1 % cross-reactivity with rhIL-2 R, rmIL-2 R and rmIL-2 R. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x106 with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry.
Restrictions For Research Use only

Handling

Product Details anti-IL15RA Antibody Target Details IL15RA Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.
Concentration Lot specific
Buffer Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.