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Mouse (Murine) IL15RA ELISA Kit

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Antigen
Interleukin 15 Receptor, alpha (IL15RA) ELISA Kits
  • AA690181
  • CD215
  • IL-15RA
  • IL15RA
Epitope
AA 33-205
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
4
3
2
Methode Type
Sandwich ELISA
Detection Range
125-8000 pg/mL
Minimum Detection Limit
125 pg/mL
Application
ELISA
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Catalog No. ABIN1889338
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN2708454 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 125-8000 pg/mL 125 pg/mL Log in to see
0.0012117105 ABIN841951 Sandwich ELISA Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2703178 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.08-20 ng/mL 0.08 ng/mL Log in to see
0.0012117105 ABIN2684588 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 125-8000 pg/mL 125 pg/mL Log in to see

Similar IL15RA ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 5 ELISA Kits

General

Antigen Interleukin 15 Receptor, alpha (IL15RA) ELISA Kits
Epitope AA 33-205
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(4), (3), (2)
Methode Type Sandwich ELISA
Detection Range 125-8000 pg/mL
Minimum Detection Limit 125 pg/mL
Application ELISA
Supplier Log in to see

Product Details IL15RA ELISA Kit

Target details Application Details Handling Images
Purpose For quantitative detection of mouse IL15RA in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse IL15RA
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse IL15RA antibod - 1
  • Lyophilized recombinant mouse IL15RA standar - 10ng/tubex2
  • Biotinylated anti- mouse IL15RA antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: G33-K205

Target details

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Antigen
Alternative Name IL15RA (IL15RA ELISA Kit Abstract)
Background Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.
Gene ID 16169
UniProt Q60819
Research Area Signaling, Apoptosis/Necrosis
Pathways JAK-STAT Signaling, TCR Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse IL15RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Monoclonal antibody from rat specific for IL15RA has been precoated onto 96-well plates. Standards(NSO, G33-K205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL15RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL15RA amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IL15RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL15RA standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 925, Standard deviation: 43.5, CV(%): 4.7
  • Sample 2: n=16, Mean(pg/ml): 3080, Standard deviation: 200.2, CV(%): 6.5
  • Sample 3: n=16, Mean(pg/ml): 5122, Standard deviation: 276.6, CV(%): 5.4,
  • Sample 1: n=24, Mean(pg/ml): 1273, Standard deviation: 65, CV(%): 5.1
  • Sample 2: n=24, Mean(pg/ml): 3377, Standard deviation: 236.4, CV(%): 7
  • Sample 3: n=24, Mean(pg/ml): 5419, Standard deviation: 341.4, CV(%): 6.3
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

Images

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Supplier Images
ELISA image for IL15RA ELISA Kit (Interleukin 15 Receptor, alpha) (ABIN1889338) Mouse IL15RA PicoKine ELISA Kit standard curve