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anti-Human IL2 antibody for ELISA

Recommended IL2 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) Antibodies
  • IL2
  • IL-2
  • il2
  • TCGF
  • lymphokine
  • Il-2
  • POIL2
  • interleukin 2
  • IL2
  • il2
  • Il2
Reactivity
Human
465
241
98
31
17
6
6
6
4
3
3
3
1
1
Host
Rabbit
314
258
212
17
5
4
Clonality
Polyclonal
Conjugate
This IL2 antibody is conjugated to Biotin
84
49
49
27
19
17
17
14
12
11
8
8
7
7
7
7
7
7
7
7
6
6
6
4
4
4
4
4
4
4
3
3
2
2
2
1
1
Application
ELISA, Western Blotting (WB)
450
385
217
86
72
49
48
48
38
35
26
23
15
10
8
7
7
6
4
4
3
3
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN1043809
$ 379.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN969214 ELISA WB Mouse IgG1 Log in to see 8D5 2
1 ABIN2869388 ELISA WB Mouse IgG1 Log in to see 8D5 2
1 ABIN2663774 FACS ELISA WB PE Rat IgG2a kappa Log in to see MQ1-17H12 10
1 ABIN1102470 IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN964771 ELISA WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN3185166 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN964758 IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1176901 ICS Neut ELISA Rat IgG2a, kappa Log in to see MQ1-17H12 4
1 ABIN4324783 ELISA FACS WB Mouse IgG2b kappa AA 22-153 Log in to see 2D5
1 ABIN1498858 ELISA WB Mouse IgG1 Log in to see 8D5
1 ABIN5518852 ELISA WB Rabbit IgG AA 21-153 Log in to see Polyclonal
1 ABIN465290 ELISA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN1102472 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1102473 IHC (p) Neut ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN465313 ELISA IHC (p) Mouse IgG1 Log in to see
1 ABIN1583031 BP IHC ELISA WB Mouse Log in to see 1
1 ABIN1830485 ELISA Biotin Mouse IgG2b, kappa Log in to see 12K270
1 ABIN966590 ELISA WB Mouse IgG1 Log in to see 8D5 2
1 ABIN2689748 ELISA Biotin Mouse IgG1, kappa Log in to see B33 1
1 ABIN2663775 FACS ELISA WB PE Rat IgG2a kappa Log in to see MQ1-17H12 10

Top referenced anti-IL2 antibody for ELISA

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Dot Blot (DB) 4 Antibodies
ELISA 258 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 5 Antibodies
Enzyme Immunoassay (EIA) 24 Antibodies
Flow Cytometry (FACS) 112 Antibodies
Functional Studies (Func) 16 Antibodies
Immunoassay (IA) 3 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 58 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies

General

Antigen Interleukin 2 (IL2) Antibodies
Reactivity Human
(465), (241), (98), (31), (17), (6), (6), (6), (4), (3), (3), (3), (1), (1)
Host Rabbit
(314), (258), (212), (17), (5), (4)
Clonality
Polyclonal
Conjugate This IL2 antibody is conjugated to Biotin
(84), (49), (49), (27), (19), (17), (17), (14), (12), (11), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Application ELISA, Western Blotting (WB)
(450), (385), (217), (86), (72), (49), (48), (48), (38), (35), (26), (23), (15), (10), (8), (7), (7), (6), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL2 Antibody

Target Details IL2 Application Details Handling References for anti-IL2 Antibody (ABIN1043809) Images
Specificity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-2 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics IL-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Purification purified
Immunogen This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-2 protein.
Immunogen Type: RecombinantProtein
Isotype IgG

Target Details IL2

Product Details anti-IL2 Antibody Application Details Handling References for anti-IL2 Antibody (ABIN1043809) Images back to top
Antigen
Alternative Name IL-2 (IL2 Antibody Abstract)
Background IL-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Synonyms: Interleukin-2, IL-2, IL2, T-cell growth factor, TCGF, Aldesleukin, Lymphokine
Gene ID 3558
NCBI Accession NP_000577
UniProt P60568
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation

Application Details

Product Details anti-IL2 Antibody Target Details IL2 Handling References for anti-IL2 Antibody (ABIN1043809) Images back to top
Application Notes This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 15 kDa in size corresponding to human IL-2 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Comment

Gene Name: IL2

Restrictions For Research Use only

Handling

Product Details anti-IL2 Antibody Target Details IL2 Application Details References for anti-IL2 Antibody (ABIN1043809) Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.1 mg/ml
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-IL2 Antibody (ABIN1043809)

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling Images back to top
Product cited in:

Bazan: "Unraveling the structure of IL-2." in: Science (New York, N.Y.), Vol. 257, Issue 5068, pp. 410-3, 1992