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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||8 Antibodies|
|Blocking Peptide (BP)||2 Antibodies|
|Dot Blot (DB)||4 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||24 Antibodies|
|Flow Cytometry (FACS)||105 Antibodies|
|Functional Studies (Func)||16 Antibodies|
|Immunoassay (IA)||3 Antibodies|
|Immunocytochemistry (ICC)||26 Antibodies|
|Immunofluorescence (IF)||17 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies|
|Immunohistochemistry (IHC)||58 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies|
|Antigen||Interleukin 2 (IL2) Antibodies|
|Epitope||AA 21-153 Alternatives|
|Conjugate||This IL2 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut)
|9 references available|
|Supplier||Log in to see|
Product Details anti-IL2 AntibodyTarget Details IL2 Application Details Handling References for anti-IL2 Antibody (ABIN4899553) Images
|Specificity||Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.|
|Purification||Protein A or G purified from hybridoma culture supernatant|
|Sterility||0.2 μm filtered|
|Endotoxin Level||<0.10 EU per 1 μg of the antibody by the LAL method.|
|Immunogen||E. coli-derived recombinant human IL-2, Ala21-Thr153, Accession # NP_000577|
Target Details IL2Product Details anti-IL2 Antibody Application Details Handling References for anti-IL2 Antibody (ABIN4899553) Images back to top
|Alternative Name||IL-2 (IL2 Antibody Abstract)|
|Background||Alternative Names: aldesleukin, IL2, IL-2lymphokine, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor, T-cell growth factor, TCGF|
|Research Area||Immunology, Cytokines, Innate Immunity|
|Pathways||JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-IL2 Antibody Target Details IL2 Handling References for anti-IL2 Antibody (ABIN4899553) Images back to top
Neutralization 0.015-0.03 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL2 Antibody Target Details IL2 Application Details References for anti-IL2 Antibody (ABIN4899553) Images back to top
|Reconstitution||Reconstitute at 0.5 mg/mL in sterile PBS.|
|Buffer||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Storage||4 °C,-20 °C,-80 °C|
|Storage Comment||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.|
|Expiry Date||12 months|
References for anti-IL2 Antibody (ABIN4899553)Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling Images back to top
|Product cited in:||
Locci, Wu, Arumemi, Mikulski, Dahlberg, Miller, Crotty: "Activin A programs the differentiation of human TFH cells." in: Nature immunology, Vol. 17, Issue 8, pp. 976-84, 2016 Method employed by authors: Biochemical Assay (BCA) (Sample species: Human).
Jiang, Chen, An, Yang, Zhang, Cheng: "Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling." in: Scientific reports, Vol. 6, pp. 32320, 2016 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).
Nata, Basheer, Cocchi, van Besien, Massoud, Jacobson, Azimi, Tagaya: "Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22338-51, 2015 Method employed by authors: Neutralization (Neut) (Sample species: Human).
Weissmüller, Semmler, Kalinke, Christians, Müller-Berghaus, Waibler: "ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation." in: Blood, Vol. 119, Issue 26, pp. 6268-77, 2012 Method employed by authors: Neutralization (Neut) (Sample species: Human).
Hutnick, Carnathan, Demers, Makedonas, Ertl, Betts: "Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive." in: Vaccine, Vol. 28, Issue 8, pp. 1932-41, 2010 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).
Withers, Fiorini, Fischer, Ettinger, Lipsky, Grammer: "T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue." in: Blood, Vol. 109, Issue 11, pp. 4856-64, 2007 Method employed by authors: Neutralization (Neut) (Sample species: Human).
Karenko, Hahtola, Päivinen, Karhu, Syrjä, Kähkönen, Nedoszytko, Kytölä, Zhou, Blazevic, Pesonen, Nevala, Nupponen, Sihto, Krebs, Poustka, Roszkiewicz, Saksela, Peterson, Visakorpi, Ranki: "Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue." in: Cancer research, Vol. 65, Issue 18, pp. 8101-10, 2005 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).
Sen, Chunsong, Baojun, Linjie, Qun, San, Qiuping, Junyan, Zhang, Jinquan: "Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells." in: Immunology, Vol. 112, Issue 2, pp. 274-89, 2004 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).
Herrero, Hu, Li, Samuels, Sharif, Kotenko, Ivashkiv: "Reprogramming of IL-10 activity and signaling by IFN-gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 10, pp. 5034-41, 2003 Method employed by authors: Neutralization (Neut) (Sample species: Human).
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