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anti-Human IL2 antibody for Immunocytochemistry

Recommended IL2 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) Antibodies
  • IL2
  • IL-2
  • il2
  • TCGF
  • lymphokine
  • Il-2
  • POIL2
  • interleukin 2
  • IL2
  • il2
  • Il2
Epitope
AA 21-153
24
20
17
17
13
13
11
10
8
5
5
4
3
2
2
2
2
2
1
1
1
1
Reactivity
Human
466
249
90
24
18
7
6
4
4
3
2
2
1
1
Host
Mouse
304
259
228
17
7
4
Clonality
Monoclonal
Conjugate
This IL2 antibody is un-conjugated
92
51
51
27
19
15
15
12
12
9
8
7
7
7
7
7
7
6
6
6
6
5
4
4
4
4
4
4
4
3
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut)
465
383
210
76
70
51
43
43
38
33
26
24
15
14
8
7
6
4
4
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN4899553
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.37591 ABIN4899552 FACS ICC Neut Mouse IgG1 AA 21-153 Log in to see 5334 9
1 ABIN1873207 ICC IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN3020909 ICC IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN3201260 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 21-153 Log in to see
1 ABIN1172183 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 21-153 Log in to see Polyclonal
1 ABIN4324832 FACS ICC IF IHC IHC (p) Alexa Fluor 405 Mouse IgG2a Log in to see N7-48A
1 ABIN4324833 FACS ICC IF IHC IHC (p) Alexa Fluor 488 Mouse IgG2a Log in to see N7-48A
1 ABIN4324834 FACS ICC IF IHC IHC (p) Alexa Fluor 647 Mouse IgG2a Log in to see N7-48A
1 ABIN4324835 FACS ICC IF IHC IHC (p) Alexa Fluor 700 Mouse IgG2a Log in to see N7-48A
1 ABIN4225296 ICC IF IHC IHC (p) Biotin Mouse IgG2a Log in to see N7-48A
1 ABIN4324836 FACS ICC IF IHC IHC (p) DyLight 650 Mouse IgG2a Log in to see N7-48A
1 ABIN4225297 FACS ICC IF IHC IHC (p) FITC Mouse IgG2a Log in to see N7-48A
1 ABIN4324837 FACS ICC IF IHC IHC (p) DyLight 680 Mouse IgG2a Log in to see N7-48A
1 ABIN4324838 FACS ICC IF IHC IHC (p) DyLight 488 Mouse IgG2a Log in to see N7-48A
1 ABIN4324839 FACS ICC IF IHC IHC (p) DyLight 755 Mouse IgG2a Log in to see N7-48A
1 ABIN4225299 FACS ICC IF IHC (p) PerCP Mouse IgG2a Log in to see N7-48A
1 ABIN4324840 FACS ICC IF IHC IHC (p) DyLight 550 Mouse IgG2a Log in to see N7-48A
1 ABIN4324841 FACS ICC IF IHC IHC (p) DyLight 350 Mouse IgG2a Log in to see N7-48A
1 ABIN4324842 FACS ICC IF IHC IHC (p) DyLight 405 Mouse IgG2a Log in to see N7-48A
1 ABIN4324798 FACS ICC IF IHC IHC (p) Mouse IgG2a Log in to see N7-48A

Top referenced anti-IL2 antibody for Immunocytochemistry

Similar anti-IL2 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 8 Antibodies
Blocking Peptide (BP) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 263 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 5 Antibodies
Enzyme Immunoassay (EIA) 24 Antibodies
Flow Cytometry (FACS) 108 Antibodies
Functional Studies (Func) 16 Antibodies
Immunoassay (IA) 3 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 21 Antibodies
Immunohistochemistry (IHC) 58 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 38 Antibodies

General

Antigen Interleukin 2 (IL2) Antibodies
Epitope AA 21-153
(24), (20), (17), (17), (13), (13), (11), (10), (8), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(466), (249), (90), (24), (18), (7), (6), (4), (4), (3), (2), (2), (1), (1)
Host Mouse
(304), (259), (228), (17), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL2 antibody is un-conjugated
(92), (51), (51), (27), (19), (15), (15), (12), (12), (9), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut)
(465), (383), (210), (76), (70), (51), (43), (43), (38), (33), (26), (24), (15), (14), (8), (7), (6), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-IL2 Antibody

Target Details IL2 Application Details Handling References for anti-IL2 Antibody (ABIN4899553) Images
Specificity Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 g of the antibody by the LAL method.
Immunogen E. coli-derived recombinant human IL-2, Ala21-Thr153, Accession # NP_000577
Clone 5334
Isotype IgG1

Target Details IL2

Product Details anti-IL2 Antibody Application Details Handling References for anti-IL2 Antibody (ABIN4899553) Images back to top
Antigen
Alternative Name IL-2 (IL2 Antibody Abstract)
Gene ID 3558
NCBI Accession NP_000577
Research Area Virology, Immunology, Cytokines, Cancer, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL2 Antibody Target Details IL2 Handling References for anti-IL2 Antibody (ABIN4899553) Images back to top
Application Notes Neutralization 0.015-0.03 μg/mL, Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells, Immunocytochemistry 8-25 μg/mL, CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-IL2 Antibody Target Details IL2 Application Details References for anti-IL2 Antibody (ABIN4899553) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 m filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 6 months, -20 to -70 C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-IL2 Antibody (ABIN4899553)

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling Images back to top
Product cited in:

Locci, Wu, Arumemi, Mikulski, Dahlberg, Miller, Crotty: "Activin A programs the differentiation of human TFH cells." in: Nature immunology, Vol. 17, Issue 8, pp. 976-84, 2016 Method employed by authors: Biochemical Assay (BCA) (Sample species: Human).

Jiang, Chen, An, Yang, Zhang, Cheng: "Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling." in: Scientific reports, Vol. 6, pp. 32320, 2016 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Nata, Basheer, Cocchi, van Besien, Massoud, Jacobson, Azimi, Tagaya: "Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22338-51, 2015 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Weissmüller, Semmler, Kalinke, Christians, Müller-Berghaus, Waibler: "ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation." in: Blood, Vol. 119, Issue 26, pp. 6268-77, 2012 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Hutnick, Carnathan, Demers, Makedonas, Ertl, Betts: "Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive." in: Vaccine, Vol. 28, Issue 8, pp. 1932-41, 2010 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Withers, Fiorini, Fischer, Ettinger, Lipsky, Grammer: "T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue." in: Blood, Vol. 109, Issue 11, pp. 4856-64, 2007 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Karenko, Hahtola, Päivinen, Karhu, Syrjä, Kähkönen, Nedoszytko, Kytölä, Zhou, Blazevic, Pesonen, Nevala, Nupponen, Sihto, Krebs, Poustka, Roszkiewicz, Saksela, Peterson, Visakorpi, Ranki: "Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue." in: Cancer research, Vol. 65, Issue 18, pp. 8101-10, 2005 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Sen, Chunsong, Baojun, Linjie, Qun, San, Qiuping, Junyan, Zhang, Jinquan: "Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells." in: Immunology, Vol. 112, Issue 2, pp. 274-89, 2004 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Herrero, Hu, Li, Samuels, Sharif, Kotenko, Ivashkiv: "Reprogramming of IL-10 activity and signaling by IFN-gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 10, pp. 5034-41, 2003 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Images

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling References for anti-IL2 Antibody (ABIN4899553) back to top
Supplier Images
Neutralization (Neut) image for anti-IL2 antibody (Interleukin 2) (AA 21-153) (ABIN4899553) Cell Proliferation Induced by IL-2 and Neutralization by Human IL-2 Antibody. Recombi...
Immunocytochemistry (ICC) image for anti-IL2 antibody (Interleukin 2) (AA 21-153) (ABIN4899553) IL-2 in Human PBMCs. IL-2 was detected in immersion fixed human peripheral blood mono...