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anti-Human IL2 antibody for Blocking Antibody

Recommended IL2 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) Antibodies
  • IL2
  • IL-2
  • il2
  • TCGF
  • lymphokine
  • Il-2
  • POIL2
  • interleukin 2
  • IL2
  • il2
  • Il2
Reactivity
Human
466
249
90
24
18
7
6
4
4
3
2
2
1
1
Host
Rat
305
259
227
17
7
4
Clonality
Monoclonal
Conjugate
This IL2 antibody is un-conjugated
92
51
51
27
19
15
15
12
12
9
8
7
7
7
7
7
7
6
6
6
6
5
4
4
4
4
4
4
4
3
2
2
2
2
1
1
Application
Blocking Antibody (Inhibition), ELISA
464
383
211
76
71
51
43
43
38
34
26
24
14
14
8
7
6
4
4
3
3
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN2689751
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN346196 Inhibition ELISA WB Goat Log in to see Polyclonal
1 ABIN1002612 Inhibition ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1002614 Inhibition IP IHC ELISA WB Mouse IgG2a Log in to see YNRhIL2
1 ABIN1002613 Inhibition ELISA WB Goat IgG Log in to see Polyclonal
1 ABIN283745 Inhibition ELISA WB Mouse IgG1 Log in to see M6110640
1 ABIN285815 Inhibition ELISA WB Goat Log in to see Polyclonal
1 ABIN289472 Inhibition ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-IL2 antibody for Blocking Antibody

Similar anti-IL2 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 8 Antibodies
Blocking Peptide (BP) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 263 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 5 Antibodies
Enzyme Immunoassay (EIA) 24 Antibodies
Flow Cytometry (FACS) 108 Antibodies
Functional Studies (Func) 16 Antibodies
Immunoassay (IA) 3 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 21 Antibodies
Immunohistochemistry (IHC) 58 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 38 Antibodies

General

Antigen Interleukin 2 (IL2) Antibodies
Reactivity Human
(466), (249), (90), (24), (18), (7), (6), (4), (4), (3), (2), (2), (1), (1)
Host Rat
(305), (259), (227), (17), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL2 antibody is un-conjugated
(92), (51), (51), (27), (19), (15), (15), (12), (12), (9), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Blocking Antibody (Inhibition), ELISA
(464), (383), (211), (76), (71), (51), (43), (43), (38), (34), (26), (24), (14), (14), (8), (7), (6), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL2 Antibody

Target Details IL2 Application Details Handling References for anti-IL2 Antibody (ABIN2689751) Images
Brand BD Pharmingen™
Characteristics The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2). The immunogen used to generate the MQ1-17H12 hybridoma was recombinant human IL-2. Unconjugated or purified forms of this antibody have been reported to be neutralizing for human IL-2 bioactivity. Expression of IL-2 by stimulated CD3+ human PBMC. Human PBMCs were stimulated for 6 hours with PMA (50 ng/mL final concentration, Sigma, Cat. #P-8139) and calcium ionophore A23187 (500-100 ng/mL final concentration, Sigma, Cat. #C-9275) in the presence of BD GolgiStop™ (2 μM final concentration, Cat. No. 554724). The PBMCs were stained with PE-Cy™5-anti-CD3 (PE-Cy™5-UCHT1, Cat. No. 555334), fixed, permeabilized, and then stained with 0.25 μg of FITC-rat anti-human IL-2 antibody (FITC-MQ1-17H12, Cat. No. 554565) using Pharmingen's staining protocol (left panel). To demonstrate specificity of staining, the binding of FITC-MQ1-17H12 was blocked by the preincubation of the fluorochrome - conjugated antibody with recombinant human IL-2 (10 ng/mL final concentration, Cat. No. 554603, middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled MQ1-17H12 antibody (5.0 μg, Cat. No. 554563, right panel) prior to staining with the FITC-MQ1-17H12 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking (middle panel) and unlabeled antibody blocking (right panel) specificity controls.

BD Pharmingen™ Purified Rat Anti-Human IL-2 - Purified - Clone MQ1-17H12 - Isotype Rat IgG2a, κ - Reactivity Hu - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human IL-2 Recombinant Protein
Clone MQ1
Isotype IgG2a, kappa

Target Details IL2

Product Details anti-IL2 Antibody Application Details Handling References for anti-IL2 Antibody (ABIN2689751) Images back to top
Antigen
Alternative Name IL-2 (IL2 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL2 Antibody Target Details IL2 Handling References for anti-IL2 Antibody (ABIN2689751) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL2 Antibody Target Details IL2 Application Details References for anti-IL2 Antibody (ABIN2689751) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-IL2 Antibody (ABIN2689751)

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling Images back to top
Product cited in:

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Andersson, Abrams, Björk, Funa, Litton, Agren, Andersson: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995

Fernandez, Andersson, Andersson, Troye-Blomberg: "Cytokine synthesis analyzed at the single-cell level before and after revaccination with tetanus toxoid." in: European journal of immunology, Vol. 24, Issue 8, pp. 1808-15, 1994