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Cat (Feline) IL2 ELISA Kit

Recommended IL2 Product (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) ELISA Kits
  • Il-2
  • IL-2
  • IL2
  • il2
  • lymphokine
  • POIL2
  • TCGF
Reactivity
Cat (Feline)
Alternatives
Kits with alternative reactivity to:
79
71
35
12
11
11
11
9
8
7
7
4
3
2
2
2
2
Methode Type
Sandwich ELISA
Detection Range
25-6000 pg/mL
Minimum Detection Limit
25 pg/mL
Application
ELISA
Supplier
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Supplier Product No.
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Catalog No. ABIN4881662
$ 1,196.58
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012043115 ABIN4881663 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 25-6000 pg/mL 25 pg/mL Log in to see
0.0012043115 ABIN2703204 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 25-6000 pg/mL 25 pg/mL Log in to see

Similar IL2 ELISA Kits

Application / Reactivity Cat (Feline)
ELISA 3 ELISA Kits

General

Antigen Interleukin 2 (IL2) ELISA Kits
Reactivity Cat (Feline)
Kits with alternative reactivity to:
(79), (71), (35), (12), (11), (11), (11), (9), (8), (7), (7), (4), (3), (2), (2), (2), (2)
Methode Type Sandwich ELISA
Detection Range 25-6000 pg/mL
Minimum Detection Limit 25 pg/mL
Application ELISA
Supplier Log in to see

Product Details IL2 ELISA Kit

Target details Application Details Handling Images
Purpose Feline IL-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
ProductDetails: Analytical Method Quantitative
Specificity This ELISA antibody pair detects feline IL-2. Approximately 3 % cross-reactivity with recombinant canine IL-2 is observed, approximately 1 % cross reactivity with recombinant porcine IL-2 and recombinant human IL-2 is observed.
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IL2 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name IL-2 (IL2 ELISA Kit Abstract)
Gene ID 751114
UniProt Q07885
Research Area Virology, Immunology, Cytokines, Cancer, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details IL2 ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Plate Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details IL2 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months