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Rat (Rattus) IL2 ELISA Kit

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Antigen
Interleukin 2 (IL2) ELISA Kits
  • IL2
  • IL-2
  • il2
  • TCGF
  • lymphokine
  • Il-2
  • POIL2
  • interleukin 2
  • IL2
  • il2
  • Il2
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
79
71
36
16
13
11
11
10
9
9
6
5
4
3
2
2
1
1
Method Type
Sandwich ELISA
Detection Range
0.1-30 ng/mL
Minimum Detection Limit
0.1 ng/mL
Application
ELISA
Options
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Catalog No. ABIN625202
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.904965 ABIN365194 Sandwich ELISA Serum, Urine, Tissue Homogenate 6.25-400 pg/mL 6.25 pg/mL Log in to see 1
10.904965 ABIN416247 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see 7
10.904965 ABIN921062 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate) 31.2-2000 pg/mL 31.2 pg/mL Log in to see 12
10.904965 ABIN2472109 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 31.25-2000 pg/mL 31.25 pg/mL Log in to see
10.904965 ABIN924837 Sandwich ELISA Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
10.904965 ABIN491777 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
10.904965 ABIN1115644 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
10.904965 ABIN1888527 Sandwich ELISA Plasma, Serum, Cell Culture Cells 0.1-30 ng/mL 0.1 ng/mL Log in to see
10.904965 ABIN2708660 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
10.904965 ABIN2708659 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
10.904965 ABIN2114295 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin - citrate) Log in to see
1 ABIN578849 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN627213 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
1 ABIN1570697 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2684654 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see 4
1 ABIN1112645 Sandwich ELISA Serum, Body Fluids, Tissue Lysate, Cell Culture Supernatant 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN5520877 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN2748852 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 31.3-2000 pg/mL 31.3 pg/mL Log in to see
1 ABIN955905 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN2114297 Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see

Top referenced IL2 ELISA Kit for ELISA

Similar IL2 ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 36 ELISA Kits

General

Antigen Interleukin 2 (IL2) ELISA Kits
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(79), (71), (36), (16), (13), (11), (11), (10), (9), (9), (6), (5), (4), (3), (2), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 0.1-30 ng/mL
Minimum Detection Limit 0.1 ng/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details IL2 ELISA Kit

Target details Application Details Handling References for IL2 Kit (ABIN625202) Images
Purpose Rat IL-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
Cross-Reactivity (Details) This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1alpha, IL-1beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, beta- NGF, TIMP-1, TNF-alpha.
Sensitivity 100 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IL2 ELISA Kit Application Details Handling References for IL2 Kit (ABIN625202) Images back to top
Antigen
Alternative Name IL-2 (IL2 ELISA Kit Abstract)
Background Interleukin-2 (IL-2) (T-cell growth factor) (TCGF)
Gene ID 116562
UniProt P17108
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation

Application Details

Product Details IL2 ELISA Kit Target details Handling References for IL2 Kit (ABIN625202) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B should be used for dilution of cell culture supernates. Suggested dilution for normal serum/plasma: 2 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water before use.
4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture supernates) into Item C vial to prepare a 100 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 150 µL IL-2 standard (100 ng/mL) from the vial of Item C, into a tube with 350 µL Assay Diluent A or 1x Assay Diluent B to prepare a 30 ng/mL standard solution. Pipette 400 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the 30 ng/mL standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 ng/mL). 200 µL 150 µL standard + 350 µL 200myl 200 µL 200 µL 200 µL 30 10 3.33 1.11 0.37 0.12 0 ng/mL ng/mL ng/mL ng/mL ng/mL ng/mL ng/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 500-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 20 µL of HRP-Streptavidin concentrate into a tube with 10 ml 1x Assay Diluent B to prepare a 500-fold diluted HRP- Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Rat IL-2 concentration (ng/mL) O D =4 50 n m 0.001 0.01 0.1 1 10 0.01 0.1 1 10 100 Assay Diluent B Rat IL-2 concentration (ng/mL) O D =4 50 n m 0.001 0.01 0.1 1 10 0.01 0.1 1 10 100
Sensitivity: The minimum detectable dose of IL-2 is typically less than 0.1 ng/mL.
Recovery: Recovery was determined by spiking Rat IL-2 into serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 98.73 88-109 Plasma 80.35 70-91 Cell culture media 78.52 67-89
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 87.7 88.3 89.4 Range ( %) 78-97 84-103 79-98 1:4 Average % of Expected 86.5 86.5 88.4 Range ( %) 77-97 80-101 78-98
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details IL2 ELISA Kit Target details Application Details References for IL2 Kit (ABIN625202) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for IL2 Kit (ABIN625202)

Product Details IL2 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Choudhary, Sheela Devi: "Longer period of oral administration of aspartame on cytokine response in Wistar albino rats." in: Endocrinologi?a y nutricio?n : o?rgano de la Sociedad Espan?ola de Endocrinologi?a y Nutricio?n, Vol. 62, Issue 3, pp. 114-22, 2015

Mohanty, Swamy, Middha, Prakash, Subbanarashiman, Maniyam et al.: "Analgesic, Anti- inflammatory, Anti- lipoxygenase Activity and Characterization of Three Bioactive Compounds in the Most Active Fraction of Leptadenia reticulata (Retz.)Wight & Arn. - A Valuable ..." in: Iranian journal of pharmaceutical research : IJPR, Vol. 14, Issue 3, pp. 933-42, 2015

Images

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Supplier Images
ELISA image for IL2 ELISA Kit (Interleukin 2) (ABIN625202) Interleukin 2 (IL2) ELISA Kit